System with buffer for lateral flow on a porous membrane
View Patent ↗Systems are described, based on a primary binding compound and a secondary binding compound used in combination with a support to detect a target in a sample. The systems includes at least one support structure, at least one small primary support portion containing at least one molecule covalently bound to a visual colloidal marker, a plurality of secondary support portions comprising secondary binding compounds that are covalently bound to the support portions and chemically active, at least one pH litmus indicator, at least one pH strip, a buffer for lateral flow on the porous membrane support that allows preservation and activity of binding compounds.
1. A system comprising
a porous membrane support structure having a lateral dimension in direction of a lateral flow and a second dimension perpendicular to the lateral dimension,
at least one primary support portion containing first binding compounds covalently bound to visual colloidal markers, capable of binding respective targets by forming first binding compound-target complexes with the respective targets,
secondary support portions spaced and staggered in the lateral dimension each comprising second binding compounds covalently bound to the porous membrane support structure, the second binding compounds configured to bind either the targets or the first binding compound-target complexes, each second binding compound of the second binding compounds specific to a different target or different first binding compound-target complex of the respective targets, the second binding compounds being different from and non-competitive with the first binding compounds for said target,
control regions spaced and staggered in the lateral dimension separate from and not overlapping with the secondary support portions and in a one-to-one correspondence with the secondary support portions such that every secondary support portion is adjacent to a corresponding control region, the control regions comprising third binding compounds configured to bind a respective first binding compound of the first binding compounds when unbound to the respective target, each control region configured to provide a control for the different targets at the secondary support portions, wherein lateral placement of each control region has a same lateral placement as its corresponding secondary support,
a pH litmus indicator and a pH control strip that is separate from and not overlapping the pH litmus indicator, wherein the pH litmus indicator and the pH control strip are both located on the porous membrane support structure in the lateral dimension downstream of the secondary support portions, wherein lateral placement of the pH control strip is such that a lateral downstream end of the pH control strip has the same lateral position as the lateral downstream end of the pH litmus indicator, and
a liquid medium for lateral flow on the support structure, configured to allow preservation and activity of binding compounds.
2. The system of claim 1 , wherein the porous membrane support structure comprises a material selected from cellulose and nylon.
3. The system of claim 2 , wherein the porous membrane support structure is portable and is configured for being hand held.
4. The system of claim 3 , wherein the visual colloidal markers comprise materials selected from a colloidal material, a colloidal gold, a colloidal magnetic particle, and colloidal quantum dots.
5. The system of claim 1 , wherein the second binding compounds are antibodies.
6. The system of claim 1 , wherein the second binding compounds are not aptamers.
7. The system of claim 1 , wherein the secondary support portions are periodically spaced with respect to each other.
8. The system of claim 1 , wherein the first binding compounds are covalently bound to the visual colloidal markers through functional groups on the first binding compounds that interact with corresponding functional groups on the colloidal markers.
9. The system of claim 1 , wherein the second binding compounds are each configured to specifically bind complex of a target of the respective targets and a first binding compound of the first binding compounds for said target.
10. The system of claim 1 , wherein each control region has a shape indicative of a target of the respective targets.
11. The system of claim 1 , wherein the porous membrane support structure is a textile strip.