Collagen scaffolds
View Patent ↗Methods for preparing and using collagen extracts and collagen scaffolds are provided. Additionally methods and related kits for the repair of articular tissue using the collagen material are provided.
1. A method of making a collagen scaffold, comprising:
preparing a neutralized collagen slurry by mixing a collagen slurry with a buffer and then with a solution containing calcium, wherein the neutralized collagen slurry has a collagen concentration of at least 10 mg/mL,
exposing the neutralized collagen slurry to a temperature sufficient to cause self-assembly of collagen fibers within the neutralized collagen slurry; and
freeze drying the neutralized collagen slurry to produce the collagen scaffold that is compressible and expandable.
2. The method of claim 1 , further comprising adjusting the pH of the neutralized collagen slurry to between 6.2 and 7.6.
3. The method of claim 1 , further comprising adding the neutralized collagen slurry to a mold.
4. The method of claim 1 , further comprising adjusting the osmolarity of the neutralized collagen slurry to between 280 and 360 osms/kg.
5. The method of claim 1 , wherein the collagen scaffold has less than about 25 μg/g of nucleic acids.
6. The method of claim 1 , wherein the collagen scaffold has less than about 5000 μg/g of phospholipids.
7. The method of claim 1 , wherein the collagen slurry includes collagen obtained from a bovine knee.