Maize event DP-004114-3 and methods for detection thereof
The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-004114-3 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.
1. A method of detecting the presence of DNA corresponding to the DP-004114-3 event in a sample, the method comprising:
(a) contacting the sample comprising maize DNA with a polynucleotide probe that hybridizes under stringent hybridization conditions with DNA from maize event DP-004114-3 and does not hybridize under said stringent hybridization conditions with a non-DP-004114-3 maize plant DNA;
(b) subjecting the sample and probe to stringent hybridization conditions; and
(c) detecting hybridization of the probe to the DNA;
wherein detection of hybridization indicates the presence of the DP-004114-3 event.
2. The method of claim 1 , wherein the probe comprises the 5′ junction sequence of event DP-004114-3.
3. The method of claim 1 , wherein the probe comprises the sequence of SEQ ID NO: 27.
4. The method of claim 1 , wherein the probe comprises the 3′ junction sequence of event DP-004114-3.
5. The method of claim 1 , wherein the probe comprises the sequence of SEQ ID NO: 28.