Sensitive and rapid methods of using chimeric receptors to identify autoimmune disease and assess disease severity
The present invention provides methods and compositions useful in the diagnosis and management of autoimmune diseases. In particular, the present invention provides improved methods and compositions for the diagnosis and management of Graves' disease. The methods of the present invention not only avoids the need for radioactivity and are much simpler, economical, and rapid than methods traditionally used for the diagnosis of Graves' disease, but also improve upon the sensitivity and detection abilities of previous luciferase-based autoantibody detection assays. Such improvements are based upon the superior performance of assays comprising a chimeric TSH receptor in the presence of a glucocorticoid including, but not limited to, dexamethasone.
1. A kit for the diagnosis of Grave's disease comprising:
i) a cell line comprising a stably transfected recombinant plasmid vector encoding a chimeric thyrotropin stimulating hormone (TSH) receptor and a reporter gene encoding a reporter molecule, wherein said TSH receptor is encoded by a nucleic acid sequence comprising SEQ ID NO: 3; and
ii) a cell culture medium compatible with the cell line;
wherein, when the cell line and the cell culture medium are combined with a serum sample derived from a patient suspected of having Grave's disease, a detectable signal is produced by the reporter molecule if a sufficient amount of a TSH receptor-specific stimulating auto-antibody is present in the serum, and an intensity of the detectable signal indicates a concentration of the TSH receptor specific stimulating auto-antibody.
2. The kit of claim 1 , wherein the concentration of the TSH receptor specific stimulating auto-antibody correlates with a clinical severity of Grave's disease.
3. The kit of claim 1 , wherein the cell culture medium contains a glucocorticoid.
4. The kit of claim 3 , wherein the glucocorticoid is selected from the group consisting of dexamethasone, prednisone, hydrocortisone, fluticasone, and cortisone.
5. The kit of claim 1 , wherein the reporter gene is a luciferase gene derived from a firefly species.
6. The kit of claim 1 , wherein the cell line is a Chinese Hamster Ovary (CHO) line.
7. The kit of claim 6 , wherein the cell line is a human Rhabdomyosarcoma (RD) line.
8. The kit of claim 1 , further comprising an instruction sheet.
9. The kit of claim 1 , wherein the cell culture medium is a Growth Medium.
10. The kit of claim 9 , wherein the cell culture medium is a Stimulation Medium.
11. The kit of claim 10 , wherein the Stimulation Medium contains polyethylene glycol.
12. The kit of claim 1 , wherein the cell culture medium contains luciferin.