Method for fabricating two-dimensional protein crystals
View Patent ↗Polypeptide assemblies and building blocks and methods for making them are provided.
1. A non-naturally occurring symmetrical polypeptide building block comprising two or more RhuA proteins, wherein each subunit of the RhuA protein comprises a cysteine residue at position 98, and wherein the RhuA proteins of the polypeptide building block are coupled by formation of disulfide-bonds between some or all of said plurality of cysteine residues of different RhuA proteins to facilitate formation of 2D crystals.
2. The polypeptide building block of claim 1 , wherein at least one of said cysteine residues has been introduced into said polypeptide building block through genetic engineering.
3. The polypeptide building block of claim 1 , wherein said polypeptide building block is generated via solid phase synthesis.
4. The polypeptide building block claim 1 , further comprising an additional polypeptide modification selected from the group consisting of functional groups, tags, other polypeptides, peptide tags, detectable labels, fluorophores, and nanoparticles.
5. The polypeptide building block of claim 4 , wherein the peptide tag comprises a His tag comprising a sequence set forth in SEQ ID NO: 22.
6. The polypeptide building block of claim 4 , wherein the peptide tag comprises a Pd4 peptide comprising a sequence set forth in SEQ ID NO: 23.
7. The polypeptide building block of claim 4 , wherein the peptide tag comprises a ACP protein comprising a sequence set forth in SEQ ID NO: 24.
8. The polypeptide building block of claim 1 , wherein the polypeptide building block is chemically modified with inorganic nanoparticles or one or more organic functional groups including a fluorophore, a metal chelating group or a host complex.
9. A 2D crystalline material comprising two or more RhuA proteins, wherein each subunit of each RhuA protein comprises a cysteine residue at position 98, wherein disulfide bonds are formed between some or all of said cysteine residues.
10. The 2D crystalline material of claim 9 , wherein said 2D crystalline material is auxetic.
11. The 2D crystalline material of claim 9 , wherein the 2D crystalline material comprises a Poisson's ratio of at least −1.
12. The 2D crystalline material of claim 9 , wherein the 2D crystalline material is chemically modified with inorganic nanoparticles or one or more organic functional groups including a fluorophore, a metal chelating group or a host complex.
13. The 2D crystalline material of claim 9 , wherein the 2D crystalline material can be genetically modified/fused with functional proteins and peptides.
14. The 2D crystalline material of claim 9 , wherein the 2D crystalline material is free of defects.
15. The 2D crystalline material of claim 9 , wherein the 2D crystalline material allows dynamic interconversion between an open conformational state and a closed conformational state.