Methods and devices for the production of decellularised tissue scaffolds
This invention relates to a method of producing a decellularised tissue scaffold which comprising treating a sample of tissue with an osmotic reagent and a detergent whilst subjecting the tissue sample to oscillation with a displacement of 1 mm or more and a frequency of 3 Hz or more during these treatment steps. This method may be useful in producing acellular scaffolds that maintain the 3-D architecture and extracellular matrix composition and morphology of the native tissue.
1. A method of producing a decellularised tissue scaffold comprising;
(i) providing a tissue sample selected from the group consisting of kidney, muscle, bone, adipose, cartilage, lung, bladder, cornea, skin, liver, spleen, placenta, intestine, pancreas, prostate, breast and heart,
(ii) treating the tissue sample with an osmotic reagent, and
(iii) treating the tissue sample obtained from step (ii) with a detergent,
wherein steps (ii) and (iii) are repeated, and wherein the tissue sample is subjected to oscillation in a single linear dimension with a displacement of 1 mm or more and a frequency of 3 to 100 Hz during steps (ii) and (iii), said oscillation subjecting the tissue sample to a g-force of 6 to 50 ms −2 thereby producing a decellularised tissue scaffold.
2. The method according to claim 1 wherein the tissue sample is subjected to oscillation at 3 to 75 Hz.
3. A method according to claim 1 wherein the oscillation has a displacement of 5 to 50 mm.
4. The method according to claim 1 wherein the osmotic agent is a hypotonic agent.
5. The method according to claim 4 wherein the hypotonic agent is deionised water.
6. The method according to claim 1 wherein the detergent comprises polyethylene glycol p-(1,1,3,3-tetramethylbutyl)-phenyl ether.
7. The method according to claim 1 wherein the detergent comprises sodium dodecyl sulfate (SDS).
8. The method according to claim 1 wherein the detergent comprises sodium deoxycholate (SdC).
9. The method according to claim 1 wherein the tissue sample is further treated with the detergent in combination with a protease.
10. The method according to claim 9 wherein the protease is trypsin.
11. The method according to claim 1 wherein the cells in the tissue sample are mechanically damaged in step (i).
12. The method according to claim 11 wherein the cells are mechanically damage by freezing and thawing the tissue sample.
13. The method according to claim 1 comprising;
(a) exposing the tissue sample obtained from step (i) to deionised water,
(b) repeating step (a),
(c) exposing the tissue sample obtained from step (c) to detergent and protease,
(d) re-exposing the tissue sample obtained from step (c) to detergent and protease,
(e) repeating step (d) 0 to 10 times,
(f) exposing the tissue sample obtained from step (e) to saline,
(g) repeating step (f) 0-10 times,
(h) repeating steps (a) to (e) 0-10 times,
(i) exposing the tissue sample obtained from step (h) to saline, and
(j) repeating step (i) 0-10 times.
14. The method according to claim 1 comprising;
(a) exposing the tissue sample obtained from step (i) to deionised water,
(b) repeating step (a) 4 times,
(c) exposing the tissue sample obtained from step (b) to detergent and protease for 2 minutes,
(d) re-exposing the tissue sample obtained from step (c) to detergent and protease for 4 minutes,
(e) repeating step (d),
(f) exposing the tissue sample obtained from step (e) to saline,
(g) repeating step (f) 4 times,
(h) repeating steps (a) to (e),
(i) exposing the tissue sample obtained from step (h) to saline, and
(j) repeating step (i) 2 times.
15. A method according to claim 1 comprising;
(a) exposing the tissue sample obtained from step (i) to deionised water,
(b) repeating step (a) 10 times,
(c) exposing the tissue sample obtained from step (b) to detergent and protease for 2 minutes,
(d) re-exposing the tissue sample obtained from step (c) to detergent and protease for 4 minutes,
(e) repeating step (d),
(f) exposing the tissue sample obtained from step (e) to saline,
(g) repeating step (f) 4 times,
(h) repeating steps (a) to (e),
(i) exposing the tissue sample obtained from step (h) to saline, and
(j) repeating step (i) 2 times.
16. A method according to claims 1 comprising;
(a) exposing the tissue sample obtained from step (i) to deionised water,
(b) repeating step (a) 11 times,
(c) exposing the tissue sample obtained from step (b) to detergent and protease for 2 minutes,
(d) re-exposing the tissue sample obtained from step (c) to detergent and protease for 4 minutes,
(e) repeating step (d),
(f) exposing the tissue sample obtained from step (e) to saline for 2 minutes,
(g) re-exposing the tissue sample obtained from step (f) to saline for 5 minutes, and
(h) repeating step (g) 2 times.
17. The method according to claim 13 wherein the tissue sample is a liver sample.
18. The method according to claim 1 comprising;
(a) exposing the tissue sample obtained from step (i) to deionised water,
(b) repeating step (a) 5 to 10 times,
(c) exposing the tissue sample obtained from step (b) to detergent,
(d) repeating step (a) 5 to 10 times with the tissue sample obtained from step (c),
(e) exposing the tissue sample obtained from step (d) to saline, and
(f) optionally repeating steps (a) to (e) one or more times.
19. The method according to claim 18 wherein the tissue sample is a kidney or heart sample.
20. The method according to claim 13 wherein the tissue sample is oscillated vertically at 50 Hz.
21. The method according to claim 1 comprising;
(a) exposing the tissue sample obtained from step (i) to deionised water,
(b) repeating step (a) 19 times,
(c) exposing the tissue sample obtained from step (b) to detergent and protease for 2 minutes,
(d) re-exposing the tissue sample obtained from step (c) to detergent and protease for 2 minutes,
(e) repeating step (d),
(f) exposing the tissue sample obtained from step (e) to saline
(g) repeating step (f) 4 times,
(h) repeating steps (a) to (e) 2 times,
(i) exposing the tissue sample obtained from step (h) to saline, and
(j) repeating step (i) 2 times.
22. The method according to claim 1 comprising;
(a) exposing the tissue sample obtained from step (i) to deionised water,
(b) repeating step (a) 9 times,
(c) exposing the tissue sample obtained from step (b) to detergent and protease for 2 minutes,
(d) re-exposing the tissue sample obtained from step (c) to detergent and protease for 4 minutes,
(e) repeating step (d)
(f) repeating steps (c) to (e),
(g) exposing the tissue sample obtained from step (f) to saline, and
(h) repeating step (g) 2 times.
23. The method according to claim 1 comprising;
(a) exposing the tissue sample obtained from step (i) to deionised water,
(b) repeating step (a) 10 times,
(c) exposing the tissue sample obtained from step (b) to detergent and protease for 2 minutes,
(d) re-exposing the tissue sample obtained from step (c) to detergent and protease for 4 minutes,
(e) repeating step (d),
(f) exposing the tissue sample obtained from step (e) to saline, and
(g) repeating step (f) 2 times.
24. The method according to claim 1 comprising;
(a) exposing the tissue sample obtained from step (i) to deionised water
(b) repeating step (a) 20 times,
(c) exposing the tissue sample obtained from step (b) to detergent and protease for 2 minutes,
(d) re-exposing the tissue sample obtained from step (c) to detergent and protease for 4 minutes,
(e) repeating step (d),
(f) exposing the tissue sample obtained from step (e) to saline,
(g) repeating step (f) 5 times,
(h) repeating steps (a) to (d) 5 times,
(i) exposing the tissue sample obtained from step (h) to saline, and
(j) repeating step (i) 2 times.
25. The method according to claim 1 comprising;
(a) exposing the tissue sample obtained from step (i) to deionised water,
(b) repeating step (a) 4 times,
(c) exposing the tissue sample obtained from step (b) to detergent and protease for 2 minutes,
(d) re-exposing the tissue sample obtained from step (c) to detergent and protease for 4 minutes,
(e) repeating step (d),
(f) exposing the tissue sample obtained from step (e) to peracetic acid (PAA),
(g) repeating step (f) once,
(h) exposing the tissue sample obtained from step (g) to ammonium hydroxide (NH 4 OH),
(i) repeating step (h) 2 times,
(j) exposing the tissue sample obtained from step (i) to deionised water,
(k) repeating step (j) 10 times,
(l) repeating steps (c) and (d) with the tissue sample obtained from step (k),
(m) exposing the tissue sample obtained from step (l) to saline, and
(n) repeating step (m) 2 times.
26. The method according to claim 21 wherein the tissue sample is a liver, pancreas or intestine sample.
27. The method according to claim 21 wherein the tissue sample is oscillated horizontally at 30 Hz.
28. The method according to claim 1 wherein the tissue sample is exposed to the osmotic agent and/or the detergent for 2 to 4 minutes in each repetition of steps (ii) and (iii).
29. The method according to claim 1 further comprising sterilising the tissue scaffold following decellularisation.
30. The method according to claim 1 further comprising repopulating the decellularised tissue scaffold with cells to produce a recellularised tissue scaffold.
31. A method for testing a compound, drug, biological agent, device or therapeutic intervention, comprising:
providing a sample of the decellularised tissue scaffold produced according to the method of claim 1 , optionally repopulating the decellularised tissue sample with cells to produce a recellularised tissue,
contacting the decellularised tissue sample or the recellularised tissue with a compound, drug, biological agent, device or therapeutic intervention, and
measuring the effect of the compound, drug, biological agent, device or therapeutic intervention on the decellularised tissue sample or the recellularised tissue.
32. A method for identifying one or more extracellular matrix biomarkers, comprising:
providing a sample of the decellularised tissue scaffold produced according to the method of claim 1 ,
contacting the decellularised tissue sample to a protease that degrades one or more extracellular matrix components,
measuring degradation of the one or more components of the decellularised tissue sample by the protease, and
identifying said one or more components degraded by the protease as extracellular matrix biomarkers.