Microbial Composition
The present invention refers to a microbial composition comprising probiotic microorganisms, adjuvants and an acceptable carrier. The composition of the invention is stable and shows efficacy in reducing the incidence of diarrhea in calves and also achieves a significant increase in their body weight.
1 ) A microbial composition comprising, as an active ingredient, at least one probiotic microorganism selected among the group consisting of Fibrobacter succinogenes, Ruminococcus Flavefaciens, Streptococcus bovis and Butytrivibrio fibrisolvens , together with adjuvants and an acceptable carrier.
2 ) A microbial composition according to claim 1 , wherein the concentration of the active ingredient in the composition ranges between 10.0% and 70.0% (w/w).
3 ) A microbial composition according to claim 1 , wherein the concentration of each of the microorganisms of the active ingredient ranges between 1×10 6 and 1×10 10 CFU/mL.
4 ) A microbial composition according to claim 1 , in the form of powder, soluble granulate, dispersible granulate, dispersible tablets, capsules, emulsion or suspension.
5 ) A microbial composition according to claim 1 , in the form of a W/O emulsion comprising an aqueous phase, an oil phase, emulsifying agents, viscous agents and pH regulating agents.
6 ) A microbial composition according to claim 5 , wherein the oil phase includes vegetable oils.
7 ) A microbial composition according to claim 5 , wherein the vegetable oil is selected from the group consisting of sunflower oil, soybean oil, corn seed oil, canola oil, olive oil, coconut oil, wheat germ oil, and blends thereof.
8 ) A microbial composition according to claim 5 , wherein the emulsifying agent is selected from the group consisting of lectin, polysorbates, sorbitan esters, noniphenol, sodium laurylsulfate, and blends thereof.
9 ) A microbial composition according to claim 5 , wherein the pH regulating agent is selected from the group consisting of phosphates, citrates, borates and carbonates.
10 ) A microbial composition according to claim 5 , wherein the viscosity agent is selected from the group consisting of polymers, gums, hydrocolloids, finely divided solids, waxes and blends thereof.
11 ) A microbial composition according to claim 5 , having the following composition:
COMPONENT
CONCENTRATION (%)
Adequate culture media
40..00
comprising probiotic
microorganisms
Sunflower oil
49.96
Polysorbate 20
1.84
Liquid lectin
5.95
Hydrocolloid
1.50
Sodium bicarbonate
0.75
12 ) A microbial composition according to claim 11 , wherein the adequate culture media comprising probiotic microorganisms has the following composition:
Component
Concentration (g/L)
Anaerobic Microorganisms
1 × 10 6 -1 × 10 10 CFU/mL
( Fibrobacter succinogenes ,
Ruminococcus Flavefaciens ,
Streptococcus bovis and
Butytrivibrio fibrisolvens )
Glucose
2.0-40.0
Yeast extract
2.0-5.0
Anaerobic indicator
0.5-2.0
Sodium bicarbonate
3.0-10.0
HCl-cysteine
0.5-3.0
Volatile fatty acids
0.2-0.6
KHPO 4
0.003-0.005
KH 2 PO 4
1.0-5.0
Ammonium sulfate
4.0-8.0
NaCl
4.0-6.0
MgSO 4
3.50-5.00
CaCl 2
0.5-1.0
13 ) A microbial composition according to claim 1 , for the prevention of neonate diarrhea and increasing body weight in calves.
14 ) The use of a composition according to claim 1 , for the prevention of neonate diarrhea and increasing body weight in calves.