Fusion polypeptide in which anti-inflammatory polypeptide and ferritin monomer fragment are bound and pharmaceutical composition for preventing and treating inflammatory diseases, containing same as active ingredient
The present invention relates to: a fusion polypeptide in which an anti-inflammatory polypeptide and a ferritin monomer fragment are bound; and a pharmaceutical composition for treating inflammatory diseases, containing the same as an active ingredient and, more specifically, to: a fusion polypeptide in which an anti-inflammatory polypeptide is fused to an N-terminus and/or a C-terminus of a ferritin monomer fragment from which a portion of a fourth loop and a fifth helix, of a human derived ferritin monomer, are removed; and a use thereof for treating inflammatory diseases. As in the above, the fusion polypeptide, which has an amino acid sequence represented by SEQ ID NO: 1 and in which an anti-inflammatory polypeptide is fused to an N-terminus and/or a C-terminus of a fragment of a human-derived ferritin monomer, can fuse two types of anti-inflammatory polypeptides, acting through different mechanisms, into a nano cage and administer the same, and thus the fusion polypeptide can exhibit an excellent effect in the treatment of inflammatory diseases including sepsis.
1. A fusion polypeptide comprising the amino acid sequence of SEQ ID NO: 14 or SEQ ID NO: 15.
2. A polynucleotide encoding the fusion polypeptide of claim 1 .
3. An expression vector comprising the polynucleotide of claim 2 .
4. A transformant transformed with the expression vector of claim 3 .
5. A protein cage comprising the fusion polypeptide of claim 1 , wherein an anti-inflammatory polypeptide protrudes outside the protein cage.
6. A pharmaceutical composition, the composition comprising the fusion polypeptide of claim 1 as an active ingredient.
7. A method for treating an inflammatory disease in a subject in need thereof, the method comprising administering an effective amount of a composition comprising the fusion polypeptide of claim 1 as an active ingredient to the subject in need thereof.
8. The method of claim 7 , wherein the inflammatory disease is selected from the group consisting of inflammatory bowel disease, diabetic eye disease, peritonitis, osteomyelitis, cellulitis, meningitis, encephalitis, pancreatitis, traumatic shock, bronchial asthma, rhinitis, sinusitis, otitis, pneumonia, gastritis, enteritis, cystic fibrosis, apoplexy, bronchitis, bronchiolitis, hepatitis, nephritis, arthritis, gout, spondylitis, Reiter's syndrome, polyarteritis nodosa, irritable vasculitis, Lou Gehrig's granulomatosis, Polymyalgia rheumatica, arthritic arteritis, calcium crystal arthropathies, pseudogout, non-articular rheumatism, bursitis, tenosynovitis, epicondylitis (tennis elbow), neuropathic joint disease (Charcot's joint), hemarthrosis, Henoch-Schonlein purpura, hypertrophic osteoarthropathy, multicentric reticulohistiocytoma, surcoilosis, hemochromatosis, sickle cell disease and other hemochromatosis, hyperlipoproteinemia, hypogammaglobulinemia, hyperparathyroidism, acromegaly, familial Mediterranean fever, Behcet's disease, systemic lupus erythematosus, relapsing fever, psoriasis, multiple sclerosis, sepsis, septic shock, acute respiratory distress syndrome, multiple organ dysfunction syndrome, chronic obstructive pulmonary disease, acute lung injury and broncho-pulmonary dysplasia.