Microcapsule material capable of reducing pollution containing polycyclic aromatic hydrocarbon, and preparation method and application thereof
The present invention discloses a microcapsule material capable of reducing pollution containing polycyclic aromatic hydrocarbons, and a preparation method and application thereof. The preparation method comprises the following steps: (1) culturing mycobacterium gilvum CP 13 in a bacteria culture liquid to obtain a bacterial broth, wherein the mycobacterium gilvum CP 13 was deposited in China General Microbiological Culture Collection Center (CGMCC) on Jul. 22, 2013 with a CGMCC number of CGMCC No. 7963; and (2) applying a calcium alginate and chitosan to encapsulate the bacterial broth in a microcapsule through layer-by-layer self-assembly to produce a microcapsule material capable of reducing pollution containing polycyclic aromatic hydrocarbons. The present invention produces a microcapsule material with the microorganic activity through layer-by-layer self-assembly, which has superior adaptability to the environment and good ability to reduce pollution containing polycyclic aromatic hydrocarbons. The microcapsule material of the present invention can be used in bioremediation of industrial wastewater containing polycyclic aromatic hydrocarbons and contaminated soil containing polycyclic aromatic hydrocarbons.
1. A method for preparing a microcapsule material capable of reducing pollution containing polycyclic aromatic hydrocarbons, the method comprising:
(1) culturing mycobacterium gilvum CP 13 in a bacteria culture liquid to obtain a bacterial broth; and
(2) encapsulating the bacterial broth in the microcapsules by applying a calcium alginate and chitosan to the bacterial broth through layer-by-layer self-assembly to produce a microcapsule material capable of reducing pollution containing polycyclic aromatic hydrocarbons.
2. The method according to claim 1 , wherein the bacteria culture liquid is nutrient broth medium, and wherein the bacteria culture liquid further comprises beef extract 3 g/L, peptone 10 g/L, NaCl 5 g/L, agar 20 g/L, and has a pH in the range of 7.0-7.2.
3. The method according to claim 1 , wherein the step (2) of encapsulating the bacterial broth in the microcapsule comprise
adding chitosan into the bacterial broth so that chitosan is deposited on a surface of bacteria,
adding the calcium alginate into the bacterial broth so that the calcium alginate is deposited on a surface of chitosan; and
repeating these steps 2-3 times, thereby encapsulating the bacterial broth in the microcapsules.
4. The method according to claim 3 , wherein concentrations of chitosan and the calcium alginate are each in a range of 0.1-2.0 g/L.