Process for the purification of biomass hydrolysate
The present invention is directed to a novel and advantageous process for the purification of biomass hydrolysate as well as the purified hydrolysate produced after the inventive process and the use of the purified hydrolysate as a fermentation medium.
1. Process for the purification of biomass hydrolysate comprising the steps
a) Providing a biomass hydrolysate, wherein the hydrolysate is prepared by adding at least one hydrolase enzyme to the biomass;
b) Adjusting the temperature of the biomass hydrolysate to a temperature selected from the range of from 50 to 95° C.;
c) Addition of at least one acid to the biomass hydrolysate;
d) Solid-liquid separation of the biomass hydrolysate-acid mixture to obtain a solid phase and a liquid phase;
e) Deionization of the liquid phase of the biomass hydrolysate-acid mixture after separation according to step d).
2. Process according to claim 1 , wherein the temperature according to step b) is selected from the range of from 65 to 90° C.
3. Process according to claim 1 , wherein the at least one acid is added until a pH of from 2.0 to 4.5 of the biomass hydrolysate is reached.
4. Process according to claim 1 , wherein the at least one acid has a pKa value of from −4.0 to 5.0.
5. Process according to claim 1 , wherein at least one adsorbent is added before or during any of steps b) or d).
6. Process according to claim 5 , wherein the at least one adsorbent is selected from the group consisting of bentonite, charcoal, activated carbon, diatomite, kieselguhr, bleaching earth, clay minerals, polymeric resins and any mixture thereof.
7. Process according to claim 1 , wherein steps b) and c) are at least partially carried out concurrently.
8. Process according to claim 7 , wherein the at least one acid is added during the adjustment of the temperature of the hydrolysate to a temperature selected from the range of from 65 to 90° C. from a temperature of 50° C. onwards.
9. Process according to claim 1 , wherein the temperature of the at least one acid is selected from 5 to 45° C. and the at least one acid is added to the biomass hydrolysate at a temperature of the hydrolysate selected from the range of from 70 to 95° C.
10. Process according to claim 9 , wherein the temperature difference between the at least one acid and the biomass hydrolysate is selected from the range of from 35 to 95%.
11. Process according to claim 1 , wherein the deionization is carried out by electrodialysis, ion exchange chromatography, membrane capacitive deionization, nanofiltration, reverse osmosis, chromatographic separation, hydrophobic chromatography, size exclusion chromatography or any combination thereof.
12. Process according to claim 11 , wherein deionization is carried out by electrodialysis followed by membrane capacitive deionization or by ion exchange chromatography.
13. Process according to claim 11 , wherein deionization is carried out by electrodialysis using at least one bipolar membrane.
14. Process according to claim 11 , wherein step c) is carried out before step b).
15. Process according to claim 1 , wherein step c) is carried out before step b).