IP Library › Granted Patent US 10,597,681
Granted Patent B2
US 10,597,681 · App. 15/758,843 · Granted Mar 24, 2020

Carbon fixation systems and methods

Inventors: Brendan Cruz Colón (Cambridge, MA); Chong Liu (Cambridge, MA); Marika Ziesack (Brookline, MA); Pamela Ann Silver (Cambridge, MA); Daniel G. Nocera (Winchester, MA)
Assignee: President and Fellows of Harvard College
C12P7/02C25B1/04C25B3/04C25B11/04C25B15/08Y02E60/366
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 10,597,681
App. No.
15/758,843
Granted
Mar 24, 2020
Kind
B2
Abstract

Systems and methods for fixing carbon using bacteria are described. In one embodiment, a system includes a reactor chamber with a solution contained therein. The solution may include hydrogen (H 2 ), carbon dioxide (C0 2 ), bioavailable nitrogen, and a chemolithoautotrophic bacteria. The system may also include a pair of electrodes that split water contained within the solution to form the hydrogen. Additionally, the system may be operated so that a concentration of the bioavailable nitrogen in the solution is below a threshold nitrogen concentration to cause the chemolithoautotrophic bacteria to produce a product.

Claims (29)

1. A system comprising:

a reactor including a reactor chamber with a solution contained therein, wherein the solution includes hydrogen (H 2 ), carbon dioxide (CO 2 ), bioavailable nitrogen, and a chemolithoautotrophic bacteria;

a pair of electrodes that split water contained within the solution to form the hydrogen, wherein the pair of electrodes comprise a cathode including a cobalt-phosphorus alloy and an anode including cobalt phosphate, and wherein the reactor is configured to maintain a concentration of the bioavailable nitrogen in the solution below a threshold nitrogen concentration to control production of a product by the chemolitoautrophic bacteria.

2. The system of claim 1 , wherein the chemolithoautotrophic bacteria is a Ralstonia eutropha bacteria.

3. The system of claim 1 , wherein the chemolithoautotrophic bacteria is resistant to reactive oxygen species.

4. The system of claim 1 , wherein the chemolithoautotrophic bacteria has been engineered to produce an alcohol.

5. The system of claim 1 , wherein the chemolithoautotrophic bacteria has been engineered to produce at least one of a fatty acid, an alkane, a polyhydroxyalkanoate, and an amino acid.

6. The system of claim 1 , wherein the solution includes a phosphate.

7. The system of claim 1 , further comprising an inlet to the reactor chamber, wherein the inlet is in fluid communication with a source of carbon dioxide that is continuously bubbled through the solution.

8. The system of claim 1 , further comprising an isolated gas volume above a surface of the solution within a head space of a reactor chamber.

9. The system of claim 8 , wherein the isolated gas volume comprises primarily carbon dioxide.

10. The system of claim 8 , wherein the reactor is configured to control a composition of the head space to maintain the concentration of the bioavailable nitrogen in the solution below the threshold nitrogen concentration.

11. The system of claim 1 , further comprising an inlet to the reactor chamber in fluid communication with a source of nitrogen gas, wherein the reactor is configured to control the concentration of the bioavailable nitrogen in the solution by bubbling the nitrogen gas through the solution.

12. The system of claim 1 , further comprising one or more sensors configured to sense a concentration of one or more gases dissolved in the solution, wherein the reactor is configured to control the concentration of the one or more gases dissolved in the solution based at least in part on the sensed concentration of the one or more gases dissolved in the solution.

13. The system of claim 12 , wherein the one or more sensors comprise at least one selected from the group of a pH monitor, a dissolved oxygen meter, and/or a gas chromatograph.

14. The system of claim 12 , wherein the reactor is configured to actively change the concentration of the one or more gases dissolved in the solution to be within a predetermined range.

15. The system of claim 12 , wherein the reactor is configured to actively change a generation rate of hydrogen by the pair of electrodes to control the concentration of hydrogen in the solution.

16. The system of claim 12 , wherein the one or more gases dissolved in the solution includes nitrogen gas dissolved in the solution.

17. A system comprising:

a reactor including a reactor chamber with a solution contained therein, wherein the solution includes hydrogen (H 2 ), carbon dioxide (CO 2 ), bioavailable nitrogen, and a chemolithoautotrophic bacteria;

a pair of electrodes that split water contained within the solution to form the hydrogen, wherein the pair of electrodes comprise a cathode including a cobalt-phosphorus alloy and an anode including cobalt phosphate.

18. The system of claim 17 , wherein the chemolithoautotrophic bacteria is a Ralstonia eutropha bacteria.

19. The system of claim 17 , wherein the chemolithoautotrophic bacteria is resistant to reactive oxygen species.

20. The system of claim 17 , wherein the chemolithoautotrophic bacteria has been engineered to produce an alcohol.

21. The system of claim 17 , wherein the chemolithoautotrophic bacteria has been engineered to produce at least one of a fatty acid, an alkane, a polyhydroxyalkanoate, and an amino acid.

22. The system of claim 17 , wherein the solution includes a phosphate.

23. The system of claim 17 , further comprising an inlet to the reactor chamber, wherein the inlet is in fluid communication with a source of carbon dioxide that is continuously bubbled through the solution.

24. The system of claim 17 , further comprising an isolated gas volume above a surface of the solution within a head space of the reactor chamber.

25. The system of claim 24 , wherein the isolated gas volume comprises primarily carbon dioxide.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 18, 2018
From: COLON, BRENDAN CRUZ; LIU, CHONG; NOCERA, DANIEL G.; SILVER, PAMELA A.; ZIESACK, MARIKA
To: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
Reel/Frame 047804/0120 →
Continuity (2)
Provisional Application 62218131 · Sep 14, 2015
Related Publication 20180265898A1 · Sep 20, 2018
Cited By (1)
US 12,570,536