Grasses with enhanced starch content
Methods and compositions for the production of monocot plants with increased starch content in stems are provided. In accordance with the invention, novel promoters and regulatory elements with specific temporal and spatial expression patterns are disclosed together with methods for the production of plants having desirable stem composition at harvest.
1. A DNA molecule comprising a DNA sequence operably linked to a heterologous transcribable polynucleotide molecule, wherein the DNA sequence is:
a) a sequence selected from the group consisting of SEQ ID NOs:1-20;
b) a sequence having at least 95 percent sequence identity to a sequence selected from the group consisting of SEQ ID NOs:1-20, and having gene-regulatory activity; or
c) a fragment comprising at least 500 contiguous nucleotides of any of SEQ ID NOs: 1-20, and having the activity of any of SEQ ID NOs:1-20.
2. The DNA molecule of claim 1 , wherein said DNA sequence has at least 97 percent sequence identity to a sequence selected from the group consisting of SEQ ID NOs:1-20.
3. The DNA molecule of claim 1 , wherein said DNA sequence has at least 99 percent sequence identity to a sequence selected from the group consisting of SEQ ID NOs:1-20.
4. The DNA molecule of claim 1 , wherein the DNA sequence comprises a sequence selected from the group consisting of SEQ ID NOs:1-20.
5. The DNA molecule of claim 1 , wherein the heterologous transcribable polynucleotide molecule comprises a sequence encoding a protein involved in starch biosynthesis.
6. The DNA molecule of claim 5 , wherein the protein involved in starch biosynthesis is an enzyme or a transcription factor.
7. The DNA molecule of claim 1 , wherein transcription of the heterologous transcribable polynucleotide molecule in a plant suppresses expression of a target coding sequence.
8. The DNA molecule of claim 7 , wherein the target coding sequence encodes a protein involved in starch breakdown.
9. The DNA molecule of claim 8 , wherein the protein involved in starch breakdown is selected from the group consisting of: glucan, water dikinase (GWD), β-amylase, and starch phosphorylase.
10. A transgenic plant, plant part, cell, or seed comprising the DNA molecule of claim 1 .
11. The transgenic plant, plant part, cell, or seed of claim 10 , wherein the plant is a monocot.
12. The transgenic plant, plant part, cell, or seed of claim 11 , wherein the plant is a C4 grass.
13. The transgenic plant, plant part, cell, or seed of claim 12 , wherein the plant is a sorghum plant.
14. A method of expressing a transcribable polynucleotide molecule comprising obtaining a transgenic plant according to claim 10 and cultivating plant, wherein the transcribable polynucleotide is expressed.
15. A method of producing a transgenic plant with increased starch content in stems post-anthesis, comprising the steps of:
a) transforming a plant cell with the DNA molecule of claim 1 , wherein the heterologous transcribable polynucleotide molecule confers increased starch content when expressed in stems;
b) regenerating a plant from the plant cell; and
c) cultivating the regenerated plant.
16. The method of claim 15 , wherein the plant is a monocot.
17. The method of claim 16 , wherein the plant is a C4 grass.
18. The method of claim 17 , wherein the plant is a sorghum plant.