Compositions and methods comprising the use of exiguobacterium acetylicum and bacillus coagluans alpha-glucanotransferase enzymes
An isolated and/or purified α-glucanotransferase from Exiguobacterium Acetylicum , recombinantly engineered variants thereof, active fragments thereof, synthetic nucleic acids encoding the α-glucanotransferase and variants thereof, host cells comprising the synthetic nucleic acids, and compositions comprising the α-glucanotransferase are provided. Methods of using the compositions include the manufacture of oligosaccharides.
1. A composition comprising at least: (i) an isolated GH70 subfamily 4 α-glucanotransferase comprising an amino acid sequence that is at least 95% identical to amino acids 31-731 of SEQ ID NO:2, and (ii) a glucooligosaccharide with α-(1→4) and α-(1→6) glycosidic linkages.
2. The composition of claim 1 , wherein the α-glucanotransferase comprises an amino acid sequence that is at least 98% identical to amino acids 31-731 of SEQ ID NO:2.
3. The composition of claim 1 , wherein the α-glucanotransferase comprises an amino acid sequence that is at least 99% identical to amino acids 31-731 of SEQ ID NO:2.
4. The composition of claim 1 , wherein the α-glucanotransferase comprises an amino acid sequence that is at least 99.5% identical to amino acids 31-731 of SEQ ID NO:2.
5. The composition of claim 1 , wherein the α-glucanotransferase comprises amino acids 31-731 of SEQ ID NO:2.
6. The composition of claim 1 , wherein the composition is in a lyophilized powder form, an encapsulated form, a coated form, a granulated form, or a liquid formulation.
7. The composition of claim 1 , further comprising a diluent.
8. A method of producing saccharide products, said method comprising contacting the α-glucanotransferase according to claim 1 with a substrate comprising a maltooligosaccharide or maltodextrin under conditions of pH 3-10 and 30-70° C. to produce at least one glucooligosaccharide with α-(1→4) and α-(1→6) glycosidic linkages.
9. The method of claim 8 , wherein the substrate comprises malto-tetraose, malto-pentaose, malto-hexaose, or maltoheptaose.
10. The method of claim 8 , wherein the substrate is comprised in a beverage or food, wherein said glucooligosaccharide with α-(1→4) and α-(1→6) glycosidic linkages is produced in the beverage or food in situ.
11. The method of claim 10 , wherein the substrate is comprised in said beverage.
12. The method of claim 10 , wherein the substrate is comprised in said food.