Self-contained anaerobic culture device with microcompartments
In one aspect, the present disclosure provides a device. The device includes a body comprising a waterproof base, a waterproof coversheet attached to the base, and a channel disposed between the base and the coversheet. The channel has a perimeter and an opening that provides liquid access to the channel. A portion of the perimeter is defined by a waterproof seal. A dry first oxygen-scavenging reagent and an indicator reagent for detecting sulfate reduction by a sulfate-reducing bacterium are disposed in the device between the base and the coversheet. The waterproof base comprises a plurality of open microcompartment structures facing the coversheet.
1. A device, comprising:
a body comprising a waterproof base, a waterproof coversheet attached to the waterproof base, and a channel disposed therebetween, the channel having a perimeter and a sealable opening that provides liquid access to the channel;
wherein a portion of the perimeter is defined by a waterproof seal; and
a dry first oxygen-scavenging reagent and an indicator reagent for detecting sulfate reduction by a sulfate-reducing bacterium are disposed in the device between the base and the coversheet;
wherein the waterproof base comprises a plurality of open microcompartment structures facing the coversheet.
2. The device of claim 1 , wherein the indicator reagent is disposed in the device adhered to the waterproof base or the waterproof coversheet.
3. The device of claim 1 , wherein the indicator reagent is the first a second oxygen-scavenging reagent.
4. The device of claim 1 , wherein the first oxygen-scavenging reagent is disposed in the device adhered to the waterproof base or the waterproof coversheet.
5. The device of claim 1 , further comprising a dry culture medium component disposed in the device between the base and the coversheet, the culture medium component selected to facilitate growth of a sulfate-reducing bacterium.
6. The device of claim 5 , wherein the culture medium component comprises an organic carbon source.
7. The device of claim 6 , wherein the organic carbon source is nonfermentable.
8. The device of claim 1 , further comprising a dry second oxygen-scavenging reagent disposed in the device between the base and the coversheet.
9. The device of claim 8 , wherein the second oxygen-scavenging reagent is disposed in the device adhered to the waterproof base or the waterproof coversheet.
10. The device of claim 1 , further comprising a reducing agent.
11. The device of claim 1 ;
wherein the waterproof base comprises a first adhesive layer disposed thereon facing the coversheet;
wherein a first dry component is adhered to the first adhesive layer;
wherein the first dry component is selected from the group consisting of the first oxygen-scavenging reagent, a second oxygen-scavenging reagent, a reducing agent, the indicator reagent, a culture medium component, and a combination of any two or more of the foregoing first dry components.
12. The device of claim 11 ;
wherein the waterproof coversheet comprises a second adhesive layer disposed thereon facing the base;
wherein a second dry component is adhered to the second adhesive layer;
wherein the second dry component is selected from the group consisting of the first oxygen-scavenging reagent, the second oxygen-scavenging reagent, the reducing agent, the indicator reagent, the culture medium component, and a combination of any two or more of the foregoing second dry components.
13. The device of claim 1 , wherein the first oxygen-scavenging reagent is water-soluble.
14. The device of claim 1 , wherein the first oxygen-scavenging reagent is selected from the group consisting of ferrous ammonium sulfate, ferrous chloride, ferrous sulfate, a ferrous iron salt, a metal sulfite, and a metal bisulfite.
15. The device of claim 8 , wherein the second oxygen-scavenging reagent is selected from the group consisting of ascorbic acid and salts thereof.
16. A method, comprising:
depositing a sample into the channel of the device of claim 1 ;
isolating separate portions of the sample into a plurality of the microcompartments, wherein isolating the separate portions comprises bonding the waterproof coversheet to the waterproof base such that a waterproof seal is formed and the separate portions are enclosed in separate microcompartments;
incubating the device for a period of time under conditions that facilitate growth of an anaerobic microorganism; and
detecting an indication of growth of the anaerobic microorganism in at least one of the separate microcompartments.
17. The method of claim 16 , further comprising depositing an aqueous liquid into the channel.
18. The method of claim 17 , wherein depositing the aqueous liquid into the channel comprises depositing a predetermined volume of the aqueous liquid.
19. The method of claim 18 , wherein depositing a predetermined volume comprises depositing about 0.1 mL to about 100 mL.
20. The method of claim 16 , wherein the method further comprises sealing the opening.