Systems and methods for making and using gel microspheres
The present invention generally relates to microfluidic droplets and, in particular, to multiple emulsion microfluidic droplets. In certain aspects, particles such as gel particles can be prepared in an aqueous carrier from aqueous droplets (or a non-aqueous carrier from non-aqueous droplets). For example, in some embodiments, double-emulsion droplets of a first fluid, surrounded by a second fluid, contained in a carrier fluid may be prepared, where the first fluid forms a gel and the second fluid is removed. For instance, the second fluid may be dissolved in the carrier fluid, or the second fluid may be hardened, then removed, for example, due to a change in pH. Other embodiments of the present invention are generally directed to kits containing such microfluidic droplets, microfluidic devices for making such microfluidic droplets, or the like.
1. A method, comprising:
providing droplets of a first fluid comprising polymer, surrounded by a second fluid, contained in a carrier fluid within a microfluidic channel;
solidifying the second fluid;
causing the polymer in the first fluid to form a gel; and
removing the solidified second fluid, thereby forming a suspension of the gel in the carrier fluid,
wherein the second fluid comprises a solvent and a second polymer,
wherein solidifying the second fluid comprises causing the solvent to enter the carrier fluid such that the second polymer forms a solid, and
wherein the microfluidic channel comprises a surface exhibiting a water contact angle of greater than about 90°, and/or a relatively hydrophilic surface exhibiting a water contact angle of less than about 90°.
2. The method of claim 1 , wherein causing the polymer to form a gel comprises applying ultraviolet light to cross-link the polymer to form the gel.
3. The method of claim 1 , wherein causing the polymer to form a gel comprises applying tetramethylethylenediamine (TEMED) to the carrier fluid, whereby the TEMED is able to diffuse into the first fluid.
4. The method of claim 1 , wherein solidifying the second fluid comprises solidifying the second fluid by altering the temperature of the second fluid.
5. The method of claim 1 , wherein the solidified second fluid is a gel.
6. The method of claim 1 , wherein removing the solidified second fluid comprises reducing the pH of the carrier fluid such that the solidified second fluid dissolves in the carrier fluid.
7. The method of claim 1 , wherein the first fluid further comprises an initiator.
8. The method of claim 1 , wherein the first fluid and the second fluid are immiscible.
9. The method of claim 1 , further comprising exposing a species to the gel to incorporate the species into the gel.
10. The method of claim 9 , comprising bonding the species to the gel.
11. A method, comprising:
providing droplets comprising solvent and polymer contained in a carrier fluid within a microfluidic channel;
causing the polymer to form a gel; and
removing the solvent from the gel, thereby forming a suspension of the gel in the carrier fluid,
wherein the solvent is able to form a separate phase when exposed to the carrier fluid, and
wherein the microfluidic channel comprises a surface exhibiting a water contact angle of greater than about 90°, and/or a relatively hydrophilic surface exhibiting a water contact angle of less than about 90°.
12. The method of claim 11 , wherein causing the polymer to form a gel comprises applying ultraviolet light to cross-link the polymer to form the gel.
13. The method of claim 11 , wherein causing the polymer to form a gel comprises applying tetramethylethylenediamine (TEMED) to the carrier fluid, whereby the TEMED is able to diffuse into the first fluid.
14. The method of claim 11 , wherein the droplets further comprise an initiator.
15. The method of claim 11 , wherein removing the solvent from the gel comprises causing the solvent to enter the carrier fluid.
16. The method of claim 11 , wherein the carrier fluid is an aqueous fluid.
17. The method of claim 11 , further comprising exposing the gel to a species to incorporate the species into the gel.
18. The method of claim 17 , comprising bonding the species to the gel.