Bi-specific antibodies for enhanced tumor selectivity and inhibition and uses thereof

1. A heterodimeric bispecific immunoglobulin molecule, comprising:

a first Fab or scFv fragment which specifically binds to EGFR,

a second Fab or scFv fragment which specifically binds to c-MET, and

an antibody hinge region, an antibody CH2 domain and an antibody CH3 domain comprising a hybrid protein-protein interaction interface domain wherein each of said interaction interface domain is formed by an amino acid segment of the CH3 domain of a first member and an amino acid segment of the CH3 domain of a second member,

wherein said protein-protein interface domain of the first chain is interacting with the protein-protein-interface of the second chain by homodimerization of a corresponding amino acid segment of the same member of an immunoglobulin superfamily within said interaction domains,

wherein a first or second engineered immunoglobulin chain has a polypeptide sequence (“AG-SEED”):

GQPFRPEVHLLPPSREEMTKNQVSLTCLARGFYPX 1 DIAVEWESNGQPENNYKTTPSR QEPSQGTT TFAVTSKLTX 2 DKSRWQQGNVFSCSVMHEALHNHYTQKX 3 ISL (SEQ ID NO:1), wherein X 1 , X 2 and X 3 may be any amino acid,

wherein the first Fab or scFv fragment comprises

a VL sequence selected from the group consisting of SEQ ID NO: 9, SEQ ID NO:10, SEQ ID NO: 44, and SEQ ID NO: 46; and

a VH sequence selected from the group consisting of SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 43, SEQ ID NO: 45;

wherein second Fab or scFv fragment comprises

a VL sequence selected from the group consisting of SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31, SEQ ID NO: 33, SEQ ID NO: 47, SEQ ID NO: 49, and SEQ ID NO: 51; and

a VH sequence selected from the group consisting of SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO: 30, SEQ ID NO: 32, SEQ ID NO: 34, SEQ ID NO: 48, SEQ ID NO: 50, and SEQ ID NO: 52.

2. The heterodimeric bispecific immunoglobulin molecule according to claim 1 , wherein

a first member of the immunoglobulin super family is IgG and a second member is IgA.

3. The heterodimeric bispecific immunoglobulin molecule according to claim 2 , wherein

X 1 is K or S,

X 2 is V or T, and

X 3 is T or S (SEQ ID NO: 2).

4. The heterodimeric bispecific immunoglobulin molecule according to claim 1 , wherein

the first or second engineered immunoglobulin chain has the polypeptide sequence (“GA-SEED”):

GQPREPQVYTLPPPSEELALNEX1VTLTCLVKGFYPSDIAVEWLQGSQELPRE

KYLTWX 2 PVX 3 DSD

GSX 4 FLYSILRVX 5 AX 6 DWKKGDTFSCSVMHEALHNHYTQKSLDR, wherein X 1 ,

X 2 , X 3 , X 4 , X 5 and X 6 may be any amino acid (SEQ ID NO: 3).

5. The heterodimeric bispecific immunoglobulin molecule according to claim 4 , wherein

X 1 is L or Q,

X 2 is A or T,

X 3 is L, V, D or T;

X 4 is F, A, D, E, G, H, K, N, P, Q, R, S or T;

X 5 is A or T, and

X 6 is E or D (SEQ ID NO: 4).

6. The heterodimeric bispecific immunoglobulin molecule according to claim 1 , wherein

the first engineered immunoglobulin chain has the polypeptide sequence (“AG-SEED”):

GQPFRPEVHLLPPSREEMTKNQVSLTCLARGFYPKDIAVEWESNGQPENNYKTTPSR QEPSQGTT TFAVTSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKTISL (SEQ ID NO: 5) and the second engineered immunoglobulin chain has the polypeptide sequence (“GA-SEED”):

GQPREPQVYTLPPPSEELALNELVTLTCLVKGFYPSDIAVEWLQGSQELPREKYLTW APVLDSDG SFFLYSILRVAAEDWKKGDTFSCSVMHEALHNHYTQKSLDR (SEQ ID NO: 6).

7. The heterodimeric bispecific immunoglobulin molecule according to claim 1 , wherein

the first engineered immunoglobulin chain comprises the polypeptide sequence (“AG-SEED”):

GQPFEPEVHTLPPSREEMTKNQVSLTCLVRGFYPSDIAVEWESNGQPENNYKT TPSRLEPSQGTT TFAVTSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSL (SEQ ID NO: 7) and the second engineered immunoglobulin chain comprises the polypeptide sequence (“GA-SEED”):

GQPREPQVYTLPPPSEELALNNQVTLTCLVKGFYPSDIAVEWESNGQPEPREK YLTWAPVLDSDG SFFLYSILRVDASRWQQGNVFSCSVMHEALHNHYTQKSLSL (SEQ ID NO: 8).

8. The heterodimeric bispecific immunoglobulin molecule according to claim 1 , wherein

the first Fab fragment binds EGFR with an K D of at least 5×10 −8 M.

9. The heterodimeric bispecific immunoglobulin molecule according to claim 1 , wherein

the second Fab fragment binds c-MET with an K D of at least 5×10 −8 M.

10. The heterodimeric bispecific immunoglobulin molecule according to claim 9 , wherein

the first Fab or scFv fragment is derived from cetuximab (C225).

11. The heterodimeric bispecific immunoglobulin molecule according to claim 1 , wherein

the first and second Fab or scFv fragments comprise at least one amino acid sequence selected from the group consisting of SEQ ID NO: 9, SEQ ID NO: 43, SEQ ID NO: 17, SEQ ID NO:18, or SEQ ID NO: 47, SEQ ID NO: 48(225 L, B10), or (225M, B10v5) SEQ ID NO: 11, SEQ ID NO: 44, SEQ ID NO: 31, SEQ ID NO: 51, SEQ ID NO:32, or (225H, F06) SEQ ID NO:45, SEQ ID NO: 46, SEQ ID NO: 29, SEQ ID NO: 49, SEQ ID NO: 30, SEQ ID NO: 50, or (225H, CS06) SEQ ID NO: 45, SEQ ID NO: 46, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 52, or (225M, CS06) SEQ ID NO: 11, SEQ ID NO: 44, SEQ ID NO: 33, SEQ ID NO: 34, and SEQ ID NO: 52.

12. The heterodimeric bispecific immunoglobulin molecule according to claim 11 , wherein

the first and second Fab or scFv fragments comprise amino acid sequences (225M, CS06) SEQ ID NO: 11, SEQ ID NO: 44, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 52, or (225H, CS06) SEQ ID NO:45, SEQ ID NO: 46, SEQ ID NO: 29, SEQ ID NO: 49, SEQ ID NO: 30, or SEQ ID NO: 50.

13. The heterodimeric bispecific immunoglobulin molecule according to claim 1 , wherein

a Fc domain interacts with FcRn.

14. The heterodimeric bispecific immunoglobulin molecule according to claim 1 , wherein

an amino acid which interacts with FcRn is derived from human IgG1.

15. The heterodimeric bispecific immunoglobulin molecule according to claim 1 , wherein

the bispecific immunoglobulin mediates antibody-dependent cellular cytotoxicity.

16. A method for producing a heterodimeric bispecific immunoglobulin molecule according to claim 1 , the method comprising:

culturing at least one host cell under conditions sufficient for heterologous expression of said heterodimeric bispecific immunoglobulin molecule, and

purifying said heterodimeric bispecific immunoglobulin molecule, wherein

said host cell comprises at least one polynucleotide which encodes at least one amino acid sequence selected from the group consisting of

SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO:17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO:20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 39, SEQ ID NO:40, SEQ ID NO: 41, SEQ ID NO:42, SEQ ID NO:43, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO: 47, SEQ ID NO: 48, SEQ ID NO: 49, SEQ ID NO: 50, SEQ ID NO:51, and SEQ ID NO: 52.

17. A heterodimeric bispecific immunoglobulin molecule obtainable by a method according to claim 16 .

18. The heterodimeric bispecific immunoglobulin molecule according to claim 1 , wherein

said heterodimeric bispecific immunoglobulin molecule is covalently coupled to at least one linker.

19. The heterodimeric bispecific immunoglobulin molecule according to claim 18 , wherein

the linker of said heterodimeric bispecific immunoglobulin molecule is coupled to a dye, radioisotope, or cytotoxin.

20. The heterodimeric bispecific immunoglobulin molecule according to claim 19 , wherein

the at least one linker is covalently coupled to at least one of the Fab or scFv light chains of said heterodimeric bispecific immunoglobulin molecule.

21. The heterodimeric bispecific immunoglobulin molecule according to claim 20 , wherein

said heterodimeric bispecific immunoglobulin molecule comprises two linkers covalently coupled to the Fab or scFv light chains of said heterodimeric bispecific immunoglobulin molecule.

22. The heterodimeric bispecific immunoglobulin molecule according to claim 21 , wherein

the Fab or scFv light chains and/or the CH3 domains and/or the CH2 domains are covalently coupled to a linker,

wherein said linker is covalently coupled to a dye, radioisotope, or cytotoxin.

23. A method of treating cancer, the method comprising:

administering, to a subject in need thereof, the heterodimeric bispecific immunoglobulin molecule according to claim 1 .

24. The method according to claim 23 , wherein

the cancer is at least one cancer selected from the group consisting of prostate cancer, breast cancer, adrenal cancer, leukemia, lymphoma, myeloma, bone and connective tissue sarcoma, a brain tumor, thyroid cancer, pancreatic cancer, pituitary cancer, eye cancer, vaginal cancer, vulvar cancer, cervical cancer, uterine cancer, ovarian cancer, esophageal cancer, stomach cancer, colon cancer, rectal cancer, liver cancer, gallbladder cancer, cholangiocarcinoma, lung cancer, testicular cancer, penal cancer, oral cancer, skin cancer, kidney cancers, Wilms' tumor and bladder cancer, metastatic (mCRC), non-resectable liver metastases, squamous cell carcinoma of the head and neck, non-small cell lung cancer (NSCLC), and head and neck squamous cell carcinoma (HNSCC).

25. A composition, comprising:

the heterodimeric bispecific immunoglobulin molecule according to claim 1 ; and

at least one further ingredient.

26. The composition according to claim 25 , which is a pharmaceutical composition.

27. A method of treating a subject in need thereof inflicted with cancer, the method comprising:

administering to said subject a therapeutically effective amount of the pharmaceutical composition according to claim 26 .

28. A method of treating cancer, the method comprising:

administering, to a subject in need thereof, the heterodimeric bispecific immunoglobulin molecule according to claim 19 .