Beta-glucosidase and uses thereof
The application relates to a polypeptide having beta-glucosidase activity, its method of production and its uses.
1. A variant polypeptide comprising substitution G142S at a position corresponding to position 142 of the polypeptide of SEQ ID NO:2, wherein the variant polypeptide has beta-glucosidase activity and at least 90% sequence identity to the polypeptide of SEQ ID NO:2.
2. The variant polypeptide according to claim 1 , which further comprises at least one substitution at a position of the polypeptide of SEQ ID NO:2 selected from positions 90, 103, 335, 485, 606, and any combination thereof.
3. The variant polypeptide according to claim 1 , wherein the variant polypeptide is less sensitive to glucose inhibition than the polypeptide of SEQ ID NO:2.
4. A composition comprising:
(i) the variant polypeptide according to claim 1 , and
(ii) a cellulase and/or a hemicellulase and/or a pectinase.
5. The composition according to claim 4 , wherein the cellulase is selected from the group consisting of a lytic polysaccharide monooxygenase, a cellobiohydrolase I, a cellobiohydrolase II, an endo-beta-1,4-glucanase, a beta-glucosidase and a beta-(1,3)(1,4)-glucanase or any combination thereof and the hemicellulase is selected from the group consisting of an endoxylanase, a beta-xylosidase, an alpha-L-arabinofuranosidase, an alpha-D-glucuronidase, an acetyl-xylan esterase, a feruloyl esterase, a coumaroyl esterase, an alpha-galactosidase, a beta-galactosidase, a beta-mannanase, a beta-mannosidase or any combination thereof.
6. The composition according to claim 4 , wherein the composition is a whole fermentation broth.
7. A method for the treatment of a substrate comprising cellulose and/or hemicellulose which method comprises contacting the substrate with
(i) the variant polypeptide according to claim 1 or
(ii) a composition comprising the variant polypeptide according to claim 1 , and a cellulase and/or a hemicellulase and/or a pectinase.
8. A method of producing a fermentation product, which method comprises:
a) treating a substrate using the method according to claim 7 to produce a material, and
b) fermenting the resulting material to produce the fermentation product.