Antibodies to pyroglutamate amyloid-β and uses thereof
The invention provides an antibody or antigen binding fragments thereof that binds to 3pE Aβ and methods of making and using the antibody or antigen binding fragment thereof, including use for formulations, administration and kits. The antibody and antigen binding fragments thereof and methods disclosed are useful for diagnosis, prognosis and treatment of Alzheimer's disease or other β-amyloid-related diseases.
1. An isolated antibody or antigen binding fragment thereof which binds to amyloid β protein having pyroglutamate at the third amino acid residue (3pE Aβ) comprising
a) a heavy chain variable region CDR1 comprising the amino acid sequence of SEQ ID NO:3,
b) a heavy chain variable region CDR2 comprising the amino acid sequence of SEQ ID NO:4,
c) a heavy chain variable region CDR3 comprising the amino acid sequence of SEQ ID NO:5,
d) a light chain variable region CDR1 comprising the amino acid sequence of SEQ ID NO: 14,
e) a light chain variable region CDR2 comprising the amino acid sequence of SEQ ID NO: 15, and
f) a light chain variable region CDR3 comprising the amino acid sequence of SEQ ID NO:16.
2. The isolated antibody or antigen binding fragment thereof of claim 1 wherein the antibody is a monoclonal antibody.
3. A pharmaceutical composition comprising the antibody or antigen binding fragment thereof of claim 1 and a pharmaceutically acceptable carrier.
4. The isolated antibody or antigen binding fragment thereof of claim 1 wherein the antibody is a humanized antibody.
5. A pharmaceutical composition comprising the antibody or antigen binding fragment thereof of claim 4 and a pharmaceutically acceptable carrier.
6. The isolated antibody or antigen binding fragment thereof of claim 1 wherein
a) the heavy chain variable region CDR1 is the amino acid sequence of SEQ ID NO:3,
b) the heavy chain variable region CDR2 is the amino acid sequence of SEQ ID NO:4,
c) the heavy chain variable region CDR3 is the amino acid sequence of SEQ ID NO:5,
d) the light chain variable region CDR1 is the amino acid sequence of SEQ ID NO: 14,
e) the light chain variable region CDR2 is the amino acid sequence of SEQ ID NO:15, and
f) the light chain variable region CDR3 is the amino acid sequence of SEQ ID NO:16.
7. The isolated antibody or antigen binding fragment thereof of claim 6 wherein the antibody is a humanized antibody.
8. A pharmaceutical composition comprising the antibody or antigen binding fragment thereof of claim 7 and a pharmaceutically acceptable carrier.
9. A recombinant cell that produces the antibody of any one of claims 4 and 7 .
10. The isolated antibody or antigen binding fragment thereof of claim 1 wherein
a) the heavy chain variable region comprises the amino acid sequence of SEQ ID NO:2; and
b) the light chain variable region comprising the amino acid sequence of SEQ ID NO:13.
11. The isolated antibody or antigen binding fragment thereof of claim 1 wherein the heavy chain variable region of the amino acid sequence that is at least 98% identical to the amino acid sequence of SEQ ID NO:2 comprises the heavy chain variable region CDR1 comprising the amino acid sequence of SEQ ID NO:3, the heavy chain variable region CDR2 comprising the amino acid sequence of SEQ ID NO:4, the heavy chain variable region CDR3 comprising the amino acid sequence of SEQ ID NO:5 and the light chain variable region of the amino acid sequence that is at least 98% identical to the amino acid sequence of SEQ ID NO: 13 comprises the light chain variable region CDR1 comprising the amino acid sequence of SEQ ID NO: 14, the light chain variable region CDR2 comprising the amino acid sequence of SEQ ID NO: 15, and the light chain variable region CDR3 comprising the amino acid sequence of SEQ ID NO:16.
12. The isolated antibody or antigen binding fragment thereof of claim 6 wherein the heavy chain variable region of the amino acid sequence that is at least 98% identical to the amino acid sequence of SEQ ID NO:2 comprises the heavy chain variable region CDR1 comprising the amino acid sequence of SEQ ID NO:3, the heavy chain variable region CDR2 comprising the amino acid sequence of SEQ ID NO:4, the heavy chain variable region CDR3 comprising the amino acid sequence of SEQ ID NO:5 and the light chain variable region of the amino acid sequence that is at least 98% identical to the amino acid sequence of SEQ ID NO: 13 comprises the light chain variable region CDR1 comprising the amino acid sequence of SEQ ID NO: 14, the light chain variable region CDR2 comprising the amino acid sequence of SEQ ID NO: 15, and the light chain variable region CDR3 comprising the amino acid sequence of SEQ ID NO:16.
13. The isolated antibody or antigen binding fragment thereof of any of claims 1 , 6 , 11 and 12 wherein the antibody competes for binding to 3pE Aβ with an antibody comprising a heavy chain of the amino acid sequence of SEQ ID NO: 1 and a light chain of the amino acid sequence of SEQ ID NO: 12.
14. An isolated antibody which binds to 3pE Aβ comprising
a) a heavy chain of the amino acid sequence of SEQ ID NO: 1; and
b) a light chain of the amino acid sequence of SEQ ID NO: 12.
15. A recombinant cell that produces the antibody of any one of claims 1 , 6 , 10 , 2 , or 14 .
16. A method of producing monoclonal antibodies according to any one of claims 1 , 6 , 10 , 2 , or 14 , comprising i) culturing the recombinant cell of claim 12 , ii) harvesting a supernatant of the recombinant cell culture of the step i), iii) screening and detecting the antibody binding to 3pE Aβ peptides in the supernatant of the step ii) by immunoassays or ELISA, and iv) harvesting and isolating the antibody binding to 3pE Aβ according to any one of claims 1 , 6 , 10 , 2 , or 14 by 3pE Aβ peptide-affinity purification methods.