Systems and methods for anti-SOX10 antibodies

1. A composition comprising a first antibody produced by the hybridoma deposited with the American Type Culture Collection (ATCC) under ATCC Patent Deposit Designation No. PTA-120969 and at least one other antibody or fragment thereof wherein said at least one other antibody or fragment thereof binds specifically to a protein selected from the group consisting of: Melanoma Antigen Recognized by T Cell 1 (“MART 1”) and Tyrosinase.

2. The composition of claim 1 wherein said first antibody and said at least one other antibody or fragment thereof bind specifically to proteins selected from the group consisting of:

SRY-related HMG-box 10 (“SOX10”) and MART-1;

SOX10 and Tyrosinase; and

SOX10 and MART-1 and Tyrosinase.

3. The composition of claim 1 wherein said first antibody and said at least one other antibody comprise monoclonal antibodies.

4. The composition of claim 1 wherein said first antibody and said at least one other antibody comprise mouse antibodies.

5. The composition of claim 1 wherein said at least one other antibody or fragment thereof is derived from a different species than said first antibody.

6. The composition of claim 1 wherein said at least one other antibody or fragment thereof is derived from a species selected from the group consisting of rabbit, goat, horse, chicken, and human.

7. The composition of claim 1 further comprising a label attached to said first antibody and said at least one other antibody or fragment thereof.

8. The composition of claim 7 wherein said label is selected from the group consisting of a radioactive element, magnetic particles, radioisotope, fluorescent dye, enzyme, toxin, signal, stain, detection enzymes, horseradish peroxidase (HRP), alkaline phosphatase (AP), beta-galactosidase, chromogens, 3,3′-diaminobenzidine, 3-amino-9-ethylcarbazole, 5-bromo-4-chloro-3-indolyl phosphate, 3,3′5,5′-tetramethylbenzidine, 5-bromo-4-chloro-3-indolyl-β-D-glucuronide, and any combination thereof.

9. A method for detecting, in a biological sample, SOX10 and said protein to which said at least one other antibody of claim 1 binds specifically; comprising the steps of contacting said biological sample with said antibodies of claim 1 and detecting the presence of said antibodies bound to protein in said biological sample.

10. The method of claim 9 wherein said biological sample comprises lung tissue, bladder tissue, breast tissue, or prostate tissue.

11. The method of claim 9 wherein said biological sample is selected from the group consisting of a normal tissue, neoplastic tissue, bladder tissue, kidney tissue, ovarian tissue, thyroid tissue, endometrial tissue, renal tissue, tonsil tissue, pancreas tissue, colon tissue, lymph node tissue, neoplastic pancreatic tissue, stomach tissue, prostate tissue, lung tissue, skin tissue; and breast tissue.

12. The method of claim 9 wherein said detecting is performed on an automated staining device.

13. The method of claim 9 wherein said detecting is selected from the group consisting of: manually detecting, automatically detecting, and image analysis.

14. The method of claim 9 wherein said detecting comprises a method selected from the group consisting of immunohistochemistry IHC of formalin fixed paraffin embedded (FFPE) tissue, IHC of frozen tissue sections, immunocytochemistry, and ELISA.

15. The method of claim 9 wherein said biological sample comprises tissue;

and wherein the method further comprises the steps of:

fixing or freezing said tissue; and

treating said fixed or frozen tissue to unmask epitopes to Sry-related HMG-Box gene 10 protein (SOX10).