Flow cytometry system with stepper flow control valve
A system, method, and apparatus are provided for flow cytometry. In one example, the flow cytometry system includes dual laser devices and dual scatter channels to measure velocity of particles in a core stream of sample fluid. The total flow rate of the sample fluid and the sheath fluid around the sample fluid is controlled, and thus held constant, by a feedback control system controlling a vacuum pump based on differential pressure across ends of a flow channel in the flow cell. A stepper flow control valves are disclosed that apply a physical fluid resistance to a flow of sheath fluid in the flow cytometer. The physical fluid resistance regulates a flow rate of the sheath fluid and thereby regulates a flow rate of sample fluid in the flow cytometer.
1. A flow cytometry system, comprising:
a first scatter channel to detect a first light scatter generated by a particle passing through a first laser beam, wherein the particle flows in a flow channel of a sample fluid;
a second scatter channel to detect a second light scatter generated by the particle flowing in the flow channel of the fluid sample and passing through a second laser beam, wherein the first laser beam and the second laser beam are separated by a first predetermined distance;
a flow cell to receive a flow of the sample fluid and a flow of a sheath fluid, wherein the flow of the sample fluid is surrounded by the flow of the sheath fluid; and
a linear resistance stepper valve to apply a physical fluid resistance to the flow of the sheath fluid, wherein the physical fluid resistance regulates a flow rate of the sheath fluid and thereby regulates a flow rate of the sample fluid, the linear resistance stepper valve comprising internal walls that form a piston bore; an inlet port to receive sheath fluid into the piston bore; an outlet port to send sheath fluid out of the piston bore; and a piston configured to travel a length into the piston bore.
2. The flow cytometry system of claim 1 , wherein:
the piston bore includes a cylindrical space having a central axis;
the piston includes a cylindrical device having a central axis; and
the central axis of the piston bore is collinear with the central axis of the piston.
3. The flow cytometry system of claim 1 , wherein:
the piston bore has a bore diameter;
the piston has a piston diameter and is configured to travel into an annular region of the piston bore, wherein the annular region is a ring-shaped space between the piston bore diameter and the piston diameter.
4. The flow cytometry system of claim 1 , wherein:
the piston has a maximum available travel distance in the piston bore.
5. The flow cytometry system of claim 4 , wherein:
the bore diameter and the piston diameter are designed such that the piston travels most of the maximum available travel distance to set the physical fluid resistance at a required amount.
6. The flow cytometry system of claim 5 , wherein:
the piston traveling most of the maximum available travel distance increases a precision of the required amount of the physical fluid resistance.
7. The flow cytometry system of claim 1 , wherein:
the bore and the internal walls can apply the physical fluid resistance to sheath fluid passing through the piston bore.
8. The flow cytometry system of claim 1 , wherein:
the physical fluid resistance is directly proportional to the length the piston travels into the piston bore.
9. A method for a flow cytometer with a linear resistance stepper valve including internal walls that form a piston bore, an inlet port to receive sheath fluid into the piston bore, an outlet port to send sheath fluid out of the piston bore; and
a piston configured to travel a length into the piston bore; the method comprising:
detecting a first light scatter generated by a particle passing through a first laser beam, wherein the particle flows in a flow channel of a sample fluid;
detecting a second light scatter generated by the particle flowing in the flow channel of the fluid sample and passing through a second laser beam, wherein the first laser beam and the second laser beam are separated by a first predetermined distance;
receiving a flow of the sample fluid and a flow of a sheath fluid, wherein the flow of the sample fluid is surrounded by the flow of the sheath fluid; and
applying a physical fluid resistance to the flow of the sheath fluid with the linear resistance stepper valve, wherein the physical fluid resistance regulates a flow rate of the sheath fluid and thereby regulates a flow rate of the sample fluid.
10. The method of claim 9 , wherein:
the piston bore includes a cylindrical space having a central axis;
the piston includes a cylindrical device having a central axis; and
the central axis of the piston bore is collinear with the central axis of the piston.
11. The method of claim 9 , wherein:
the piston bore has a bore diameter;
the piston has a piston diameter and is configured to travel into an annular region of the piston bore, wherein the annular region is a ring-shaped space between the piston bore diameter and the piston diameter.
12. The method of claim 9 , wherein:
the piston has a maximum available travel distance in the piston bore.
13. The method of claim 12 , wherein:
the bore diameter and the piston diameter are designed such that the piston travels most of the maximum available travel distance to set the physical fluid resistance at a required amount.
14. The method of claim 13 , wherein:
the piston traveling most of the maximum available travel distance increases a precision of the required amount of the physical fluid resistance.
15. The method of claim 9 , wherein:
the bore and the internal walls can apply the physical fluid resistance to sheath fluid passing through the piston bore.
16. The method of claim 9 , wherein:
the physical fluid resistance is directly proportional to the length the piston travels into the piston bore.