Biomarkers for the early detection of breast cancer
The present invention provides reagents and methods for breast cancer detection.
1. A method for treating breast cancer, comprising;
administering a therapeutic agent to the subject to treat the breast cancer, wherein the subject is identified as having breast cancer by
(i) contacting a bodily fluid sample obtained from the subject with a PDCD6IP (SEQ ID NO: 21) polypeptide and a HOXD1 (SEQ ID NO: 7) polypeptide and one or more additional isolated polypeptides selected from the group consisting of ATP6AP1 (SEQ ID NO: 13), DBT (SEQ ID NO: 25), CSNK1E (SEQ ID NO: 9), FRS3 (SEQ ID NO: 3), RAC3 (SEQ ID NO: 15), SF3A1 (SEQ ID NO: 1), CTBP1 (SEQ ID NO: 29), C15orf48 (SEQ ID NO: 35), MYOZ2 (SEQ ID NO: 33), EIF3E (SEQ ID NO: 39), BAT4 (SEQ ID NO: 5), ATF3 (SEQ ID NO: 19), BMX (SEQ ID NO: 45), RAB5A (SEQ ID NO: 23), UBAP1 (SEQ ID NO: 47), SOX2 (SEQ ID NO: 31), GPR15 7 (SEQ ID NO: 43), BDNF (SEQ ID NO: 17), ZMYM6 (SEQ ID NO: 41), SLC33A1 (SEQ ID NO: 11), TRIM32 (SEQ ID NO: 37), ALG10 (SEQ ID NO: 27), TFCP2 (SEQ ID NO: 49), SERPINH1 (SEQ ID NO: 51), SELL (SEQ ID NO: 55), ZNF510 (SEQ ID NO: 53), or antigenic fragments thereof;
wherein the contacting occurs under conditions whereby antibodies, if present in the bodily fluid, selectively bind to the polypeptides and
(ii) detecting presence of antibodies to the polypeptides in the bodily fluid sample.
2. The method of claim 1 , wherein the one or more additional isolated polypeptides comprises ATP6AP1 (SEQ ID NO: 13), or an antigenic fragment thereof.
3. The method of claim 1 wherein the subject has one or more of a lump in the breast tissue, lymph nodes, or armpit; changes in breast size or shape, skin dimpling, nipple inversion, spontaneous single-nipple discharge, a family/personal history of breast cancer, or the subject is a carrier of a mutation in the BRCA or other gene that predisposes the subject to breast cancer.
4. The method of claim 1 wherein the bodily fluid comprises serum or plasma.
5. The method of claim 1 wherein the subject further tested for the presence of breast cancer using a technique selected from the group consisting of mammography, biopsy, or magnetic resonance imaging.