Immunization of avians by administration of non-replicating vectored vaccines
The present invention relates generally to the fields of immunology and vaccine technology. More specifically, the invention relates to recombinant human adenovirus vectors for delivery of avian immunogens and antigens, such as avian influenza into avians. The invention also provides methods of introducing and expressing an avian immunogen in avian subjects, including avian embryos, as well as methods of eliciting an immunogenic response in avian subjects to avian immunogens.
1. A pharmaceutical composition for inducing an immune response against at least one avian immunogen of interest in an avian following in ovo administration of the composition into an amnion-allantois space of an embryonated avian egg, the pharmaceutical composition comprising:
a pharmaceutical dosage comprising a pharmaceutically acceptable carrier, excipient or diluent admixed with a recombinant human adenovirus expression vector, the recombinant human adenovirus expression vector comprising a promoter sequence operably linked to a foreign sequence encoding one or more avian antigens or immunogens of interest under the transcriptional control of the human cytomegalovirus (CMV) promoter; and,
a robotic injector for administration of the composition into the amnion-allantois space of the embryonated avian egg,
wherein the composition is configured for administration into the amnion-allantois space of an embryonated avian egg to obtain expression of the one or more avian antigens or immunogens in the avian embryo to induce the immune response in the avian.
2. The pharmaceutical composition of claim 1 , wherein the dosage is in a liquid form.
3. The pharmaceutical composition of claim 1 , wherein the dosage has been reconstituted from a lyophilized dosage with a pharmaceutical acceptable liquid.
4. The pharmaceutical composition of claim 1 , wherein the dosage further comprises an adjuvant or immunomodulatory molecules.
5. The pharmaceutical composition of claim 1 , wherein the adenovirus expression vector is E1 and/or E3 defective adenovirus serotype 5 (Ad5).
6. The pharmaceutical composition of claim 1 , wherein the one or more avian antigens or immunogens of interest are derived from avian influenza virus, infectious bursal disease virus, Marek's disease virus, avian herpesvirus, infectious laryngotracheitis virus, avian infectious bronchitis virus, avian reovirus, avipox, fowlpox, canarypox, pigeonpox, quailpox, and dovepox, avian polyomavirus, Newcastle Disease virus, avian pneumovirus, avian rhinotracheitis virus, avian reticuloendotheliosis virus, avian retroviruses, avian endogenous virus, avian erythroblastosis virus, avian hepatitis virus, avian anemia virus, avian enteritis virus, Pacheco's disease virus, avian leukemia virus, avian parvovirus, avian rotavirus, avian leukosis virus, avian musculoaponeurotic fibrosarcoma virus, avian myeloblastosis virus, avian myeloblastosis-associated virus, avian myelocytomatosis virus, avian sarcoma virus, or avian spleen necrosis virus.
7. The pharmaceutical composition of claim 1 , wherein the foreign sequence encoding the one or more avian antigens or immunogens of interest is derived from avian influenza, wherein the avian influenza antigens or immunogens are selected from the group consisting of hemagglutinin, nucleoprotein, matrix, or neuraminidase.
8. The pharmaceutical composition of claim 1 , wherein the human adenovirus comprises sequences derived from replication-defective adenovirus, non-replicating adenovirus, replication-competent adenovirus, or wild-type adenovirus.
9. The pharmaceutical composition of claim 1 , wherein a per egg dose comprises about 1×10 8 to about 1×10 11 of the adenovirus expression vector expressing one or more avian influenza antigens, or an equivalent dose measured by ifu or an equivalent dose measured by virus particles.
10. The pharmaceutical composition of claim 1 , wherein a per egg dose comprises about 5×10 10 pfu or about 2×10 8 ifu or about 3×10 10 viral particles of the adenovirus expression vector expressing one or more avian influenza antigens.
11. The pharmaceutical composition of claim 1 , wherein the avian is a chicken.
12. The pharmaceutical composition of claim 1 , wherein the one or more avian influenza antigens are selected from hemagglutinin, nucleoprotein, matrix, or neuraminidase.
13. The pharmaceutical composition of claim 1 , wherein the dosage is in the form of a suspension or a solution.