Active substances of
The present invention discloses the Cordyceps cicadae mycelia, the active substances of C. cicadae mycelia, its submerged fermentation product and its pharmaceutical composition for preventing, delaying or treating cataracts, and the methods for preparing the products above. The C. cicadae mycelia, the active substances of C. cicadae mycelia, its submerged fermentation product and its pharmaceutical composition further can be prepared as a health food for preventing or delaying cataracts.
1. A method for reducing formation of cataracts induced by UVB damage in a subject, comprising:
administering to the subject a therapeutically effective amount of a water or alcohol extract of Cordyceps cicadae.
2. The method according to claim 1 , wherein the water or alcohol extract of the Cordyceps cicadae is prepared by a method comprising:
(a) inoculating a C. cicadae mycelium on an agar plate to be incubated;
(b) inoculating the incubated mycelium in step (a) into a first medium on a first scale to be incubated;
(c) inoculating the incubated mycelium in step (b) into a second medium on a second scale to be incubated to obtain a fermented product, wherein the second scale is larger than the first scale;
(d) desiccating the fermented product, by using one being selected from the group consisting of a spray drying, a heated-air drying, a roller drying, a freeze drying and a combination thereof, to obtain a dried product; and
(e) extracting the dried product with one of a water and an alcohol to correspondingly obtain the water or alcohol extract of the Cordyceps cicadae.
3. The method according to claim 1 , wherein the subject suffers from the cataracts.
4. The method according to claim 2 , wherein the incubation in step (a) is performed at 15˜35° C. for 5˜14 days.
5. The method according to claim 2 , wherein the incubation in step (b) is performed at 15˜35° C., pH 2˜8 and 10˜250 rounds per minutes (rpm) for a plurality of days.
6. The method according to claim 2 , wherein the incubation in step (c) is performed in a fermentation tank having a tank pressure of 0.5˜1.0 kg/cm 2 , at 15˜35° C., pH 2˜8 and one of a first stirring rate and a second stirring rate, and a gas is introduced into the fermentation tank at an aeration rate of 0.01˜1.5 volume per volume per minute (vvm), wherein the first stirring rate is 10˜250 rpm, and the second stirring rate is 0 rpm.