Disposable bioreactor system
A cylindrical or annular polymeric bioreactor is disclosed which provides enhanced mixing and aeration of the growth medium while simultaneously offering reduced mechanical shear force.
1. An apparatus comprising:
(a) a hollow toroidal one-piece polymeric bioreactor vessel comprising:
at least one port configured to receive at least one sensor to monitor a bioprocess carried out in said hollow toroidal one-piece polymeric bioreactor vessel;
textured surface arranged on an inner surface of the hollow toroidal one-piece polymeric bioreactor vessel, wherein a sinusoidal shape pattern of the textured surface comprises a plurality of upstanding protuberances; and
a multitude of tubes coupled to the inner surface and extending through the plurality of upstanding protuberances of the textured surface, wherein the multitude of tubes are configured for sparging gas into said hollow toroidal one-piece polymeric bioreactor vessel; and
(b) a drive including a motor driven platform, wherein when in operation the hollow toroidal one-piece polymeric bioreactor vessel rests on the motor driven platform and the platform moves orbitally such that there is build-up of a resonant frequency traveling wave of fluid orbiting in one direction in an interior of the hollow toroidal one-piece polymeric bioreactor vessel when a particular amplitude and orbital speed is imparted to said hollow toroidal one-piece polymeric bioreactor vessel, wherein the resonant frequency depends on size and shape of the hollow toroidal one-piece polymeric bioreactor vessel, wherein the hollow toroidal one-piece polymeric bioreactor vessel was sterilized by gamma radiation, and wherein when in operation the textured surface including the multitude of tubes sparging gas into said hollow toroidal one-piece polymeric bioreactor vessel and the traveling wave of fluid induced by the orbital movement of the hollow toroidal one-piece polymeric bioreactor vessel are configured to enhance aeration and mixing efficiency on the inner surface of the hollow toroidal one-piece polymeric bioreactor vessel.
2. The apparatus in accordance with claim 1 , wherein the textured surface is located on a bottom of the hollow toroidal one-piece polymeric bioreactor vessel.
3. The apparatus in accordance with claim 2 , wherein said hollow toroidal one-piece polymeric bioreactor vessel includes at least one exit port for removal of gaseous by-products.
4. The apparatus in accordance with claim 1 , wherein the sinusoidal shape pattern of the textured surface is formed of pre-molded baffles.
5. The apparatus in accordance with claim 1 , wherein the hollow toroidal one-piece polymeric bioreactor vessel is fabricated from a rigid plastic.
6. The apparatus in accordance with claim 1 , wherein the hollow toroidal one-piece polymeric bioreactor vessel is fabricated from a flexible plastic.
7. The apparatus in accordance with claim 1 , further comprising means for illuminating contents of said hollow toroidal one-piece polymeric bioreactor vessel.
8. The apparatus in accordance with claim 7 , wherein said means for illuminating the contents of said hollow toroidal one-piece polymeric bioreactor vessel comprises at least one LED.
9. The apparatus in accordance with claim 1 , wherein the drive is further configured for simultaneously both orbiting and rocking said hollow toroidal one-piece polymeric bioreactor vessel.
10. The apparatus in accordance with claim 1 , further comprising a plurality of input ports for inserting a sensors or process feedstock inputs into the hollow toroidal one-piece polymeric bioreactor vessel.
11. The apparatus in accordance with claim 1 , wherein the at least one sensor is a pre-inserted and pre-calibrated sensor.
12. The apparatus in accordance with claim 1 , wherein the drive is configured to move the hollow toroidal one-piece polymeric bioreactor vessel in a reciprocating motion to cause the fluid to orbit in a reversed direction.
13. An apparatus comprising:
(a) a hollow toroidal one-piece polymeric bioreactor vessel comprising:
at least one port configured to receive at least one sensor to monitor a bioprocess carried out in said hollow toroidal one-piece polymeric bioreactor vessel;
a textured surface arranged on an inner bottom surface of the hollow toroidal one-piece polymeric bioreactor vessel, wherein a sinusoidal shape pattern of the textured surface comprises a plurality of upstanding protuberances; and
a multitude of tubes extending through the inner bottom surface for sparging gas into said hollow toroidal one-piece polymeric bioreactor vessel; and
(b) a drive including a motor driven platform, wherein when in operation the hollow toroidal one-piece polymeric bioreactor vessel rests on the motor driven platform and the platform moves orbitally such that there is build-up of a resonant frequency traveling wave of fluid orbiting in one direction in an interior of the hollow toroidal one-piece polymeric bioreactor vessel when a particular amplitude and orbital speed is imparted to said hollow toroidal one-piece polymeric bioreactor vessel, wherein the resonant frequency depends on size and shape of the hollow toroidal one-piece polymeric bioreactor vessel, wherein the hollow toroidal one-piece polymeric bioreactor vessel was sterilized by gamma radiation, wherein when in operation the textured surface, the multitude of tubes sparging gas into said hollow toroidal one-piece polymeric bioreactor vessel, and the traveling wave of fluid induced by the orbital movement of the hollow toroidal one-piece polymeric bioreactor vessel are configured to enhance aeration and mixing efficiency on the inner bottom surface of the hollow toroidal one-piece polymeric bioreactor vessel.
14. The apparatus in accordance with claim 13 , wherein the at least one sensor is a pre-inserted and pre-calibrated sensor.