Immunostimulatory compositions, particles, and uses related thereto
In some embodiments, described herein is a method of tumor treatment or tumor vaccination. The method generally comprises applying to a human being in need thereof a tumor therapeutic composition or tumor vaccine defined herein. The tumor therapeutic composition or tumor vaccine can be produced by protein transfer of glycosyl-phosphatidylinositol (GPI)-anchored immunostimulatory or costimulatory molecules.
1. A method of treating breast cancer comprising administering an effective amount of irradiated cells in combination with an anti-PD-1 antibody to a subject in need thereof, wherein the cells comprise:
A B7-1 molecule anchored to a lipid membrane on the exterior of the cells;
A HER-2 molecule on the exterior of the cells;
And
An IL-12 anchored to the lipid membrane on the exterior of the cells, wherein the cells are transfected with a vector comprising SEQ ID NO:7.
2. The method of claim 1 , wherein one or more of the B7-1, HER-2 and IL-12 is anchored to the lipid membrane through a conjugated glycosyl-phosphatidylinositol.
3. The method of claim 1 , wherein all of the B7-1, HER-2 and IL-12 are anchored to the lipid membrane through a conjugated glycosyl-phosphatidylinositol.
4. The method of claim 1 , wherein the cells are breast tumor cells.
5. The method of claim 1 , wherein the anti-PD-1 antibody is nivolumab.