Polypeptides Having Xylanase Activity and Polynucleotides Encoding Same
The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
1 - 24 . (canceled)
25 . A process for degrading a cellulosic material or xylan-containing material, comprising: treating the cellulosic material or xylan-containing material with one or more enzymes comprising a polypeptide having xylanase activity selected from the group consisting of
(a) a polypeptide having at least 85% sequence identity to the sequence of amino acids 18 to 364 of SEQ ID NO: 2 or amino acids 17 to 389 of SEQ ID NO: 4; and
(b) a fragment of the sequence of amino acids 18 to 364 of SEQ ID NO: 2 or amino acids 17 to 389 of SEQ ID NO: 4 that has xylanase activity.
26 . The process of claim 25 , wherein the one or more enzymes further comprise a cellobiohydrolase, an endoglucanase, a beta-glucosidase, and a GH61 polypeptide.
27 . The process of claim 25 , wherein the polypeptide has at least 85% sequence identity to the sequence of amino acids 18 to 364 of SEQ ID NO: 2 or amino acids 17 to 389 of SEQ ID NO: 4.
28 . The process of claim 25 , wherein the polypeptide has at least 90% sequence identity to the sequence of amino acids 18 to 364 of SEQ ID NO: 2 or amino acids 17 to 389 of SEQ ID NO: 4.
29 . The process of claim 25 , wherein the polypeptide has at least 95% sequence identity to the sequence of amino acids 18 to 364 of SEQ ID NO: 2 or amino acids 17 to 389 of SEQ ID NO: 4.
30 . The process of claim 25 , wherein the polypeptide has at least 97% sequence identity to the sequence of amino acids 18 to 364 of SEQ ID NO: 2 or amino acids 17 to 389 of SEQ ID NO: 4.
31 . The process of claim 25 , wherein the polypeptide comprises the sequence of amino acids 18 to 364 of SEQ ID NO: 2 or amino acids 17 to 389 of SEQ ID NO: 4.
32 . The process of claim 25 , wherein the polypeptide is a variant of the sequence of amino acids 18 to 364 of SEQ ID NO: 2 or amino acids 17 to 389 of SEQ ID NO: 4 comprising a substitution, deletion and/or insertion at one or more positions and wherein the variant has at least 95% sequence identity to the sequence of amino acids 18 to 364 of SEQ ID NO: 2 or amino acids 17 to 389 of SEQ ID NO: 4.
33 . The process of claim 25 , wherein the polypeptide is a fragment of SEQ ID NO: 2 or SEQ ID NO: 4, wherein the fragment has xylanase activity.
34 . The process of claim 25 , wherein the polypeptide is encoded by a polynucleotide that hybridizes under very high stringency conditions with the complement of the sequence of nucleotides 52 to 1165 of SEQ ID NO: 1 or nucleotides 49 to 1705 of SEQ ID NO: 3, wherein the very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide, and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 70° C.
35 . A process for producing a fermentation product, comprising:
(a) saccharifying a cellulosic material or xylan-containing material with one or more enzymes comprising a polypeptide having xylanase activity selected from the group consisting of (i) a polypeptide having at least 85% sequence identity to the sequence of amino acids 18 to 364 of SEQ ID NO: 2 or amino acids 17 to 389 of SEQ ID NO: 4; and (ii) a fragment of the sequence of amino acids 18 to 364 of SEQ ID NO: 2 or amino acids 17 to 389 of SEQ ID NO: 4 that has xylanase activity; and
(b) fermenting the saccharified cellulosic material or xylan-containing material with one or more fermenting microorganisms to produce the fermentation product.
36 . The process of claim 35 , wherein the one or more enzymes further comprise a cellobiohydrolase, and endoglucanase, a beta-glucosidase, and a GH61 polypeptide.
37 . The process of claim 35 , wherein the polypeptide has at least 90% sequence identity to the sequence of amino acids 18 to 364 of SEQ ID NO: 2 or amino acids 17 to 389 of SEQ ID NO: 4.
38 . The process of claim 35 , wherein the polypeptide has at least 95% sequence identity to the sequence of amino acids 18 to 364 of SEQ ID NO: 2 or amino acids 17 to 389 of SEQ ID NO: 4.
39 . The process of claim 35 , wherein the polypeptide has at least 97% sequence identity to the sequence of amino acids 18 to 364 of SEQ ID NO: 2 or amino acids 17 to 389 of SEQ ID NO: 4.
40 . The process of claim 35 , further comprising recovering the fermentation product from the fermentation.
41 . A process for degrading a cellulosic material or xylan-containing material, comprising: treating the cellulosic material or xylan-containing material with one or more enzymes comprising a polypeptide having xylanase activity which comprises a catalytic domain having at least 90% sequence identity to the sequence of amino acids 18 to 364 of SEQ ID NO: 2 or amino acids 17 to 326 of SEQ ID NO: 4.
42 . The process of claim 41 , wherein the one or more enzymes further comprise a cellobiohydrolase, an endoglucanase, a beta-glucosidase, and a GH61 polypeptide.
43 . The process of claim 41 , wherein the polypeptide having xylanase activity which comprises a catalytic domain has at least 95% sequence identity to the sequence of amino acids 18 to 364 of SEQ ID NO: 2 or amino acids 17 to 326 of SEQ ID NO: 4
44 . The process of claim 41 , wherein the polypeptide having xylanase activity which comprises a catalytic domain has at least 97% sequence identity to the sequence of amino acids 18 to 364 of SEQ ID NO: 2 or amino acids 17 to 326 of SEQ ID NO: 4
45 . The process of claim 41 , wherein the polypeptide having xylanase activity which comprises a catalytic domain comprises the sequence of amino acids 18 to 364 of SEQ ID NO: 2 or amino acids 17 to 326 of SEQ ID NO: 4.
46 . The process of claim 41 , wherein the polypeptide further comprises a cellulose binding domain.
47 . A recombinant host cell transformed with a nucleic acid construct or expression vector operably linked to one or more control sequences that direct the production of the polypeptide, wherein the polynucleotide is operably linked to one or more control sequences that direct the production of the polypeptide in an expression host and wherein the polypeptide is selected from the group consisting of
(a) a polypeptide having at least 85% sequence identity to the sequence of amino acids 18 to 364 of SEQ ID NO: 2 or amino acids 17 to 389 of SEQ ID NO: 4; and
(b) a fragment of the sequence of amino acids 18 to 364 of SEQ ID NO: 2 or amino acids 17 to 389 of SEQ ID NO: 4 that has xylanase activity.
48 . A method of producing a polypeptide having xylanase activity, comprising cultivating the recombinant host cell of claim 47 under conditions conducive for production of the polypeptide; and
49 . The method of claim 48 , further comprising recovering the polypeptide.