Carbapenemases for use with antibiotics for the protection of the intestinal microbiome
This invention relates, in part, to various compositions and methods for protecting the gastrointestinal microbiome from antibiotic disruption.
1. A method for producing a recombinant carbapenemase in Escherichia coli ( E. coli ), comprising:
(a) providing a host E. coli cell transformed with a vector comprising a sequence encoding the carbapenemase;
(b) culturing the E. coli cell to induce expression of the carbapenemase; and
(c) recovering the carbapenemase from a soluble fraction prepared from the cytoplasm or periplasmic space of the E. coli cell, wherein:
the carbapenemase comprises at least 95% sequence identity with the amino acid sequence of SEQ ID NO: 68 (P2A), and
the culturing is in the presence of about 100 μM zinc, such that the amount of carbapenemase in the soluble fraction is increased and the amount of carbapenemase in inclusion bodies is reduced, relative to culturing in the absence of zinc.
2. The method of claim 1 , wherein the carbapenemase has the amino acid sequence of SEQ ID NO: 68 (P2A).
3. The method of claim 1 , wherein the method yields about 10 grams of active carbapenemase per liter of culture.
4. The method of claim 1 , wherein the method yields about 15 grams of active carbapenemase per liter of culture.
5. The method of claim 1 , wherein the zinc is in the form of ZnSO 4 .
6. The method of claim 1 , wherein the culturing is in a bioreactor or a shake flask.
7. The method of claim 1 , wherein the E. coli cell is induced to express the carbapenemase using isopropyl β-D-1-thiogalactopyranoside (IPTG).
8. A method for producing a recombinant carbapenemase in Escherichia coli ( E. coli ), comprising:
(a) providing a host E. coli cell transformed with a vector comprising a sequence encoding the carbapenemase;
(b) culturing the E. coli cell to induce expression of the carbapenemase; and
(c) recovering the carbapenemase from a soluble fraction prepared from the cytoplasm or periplasmic space of the E. coli cell, wherein:
the carbapenemase comprises at least 95% sequence identity with the amino acid of SEQ ID NO: 53 (NDM-1), and
the culturing is in the presence of about 100 μM zinc, such that the amount of carbapenemase in the soluble fraction is increased and the amount of carbapenemase in inclusion bodies is reduced, relative to culturing in the absence of zinc.
9. The method of claim 8 , wherein the carbapenemase has the amino acid sequence of SEQ ID NO: 53 (NDM-1).
10. The method of claim 8 , wherein the method yields about 10 grams of active carbapenemase per liter of culture.
11. The method of claim 8 , wherein the method yields about 15 grams of active carbapenemase per liter of culture.
12. The method of claim 8 , wherein the zinc is in the form of ZnSO 4 .
13. The method of claim 8 , wherein the culturing is in a bioreactor or a shake flask.
14. The method of claim 8 , wherein the E. coli cell is induced to express the carbapenemase using isopropyl β-D-1-thiogalactopyranoside (IPTG).