System for hypothermic transport of samples
A system for the hypothermic transport of biological samples, such as tissues, organs, or body fluids. The system includes a self-purging preservation apparatus to suspend a sample in preservation fluid and perfuse a tissue with preservation fluid. The self-purging preservation apparatus is placed in an insulated transport container having a cooling medium. When assembled, the system allows for transport of biological samples for extended periods of time at a stable temperature.
1. A transport container comprising:
a sample storage chamber configured to receive a biological sample;
an adapter for coupling the biological sample inside the storage chamber;
a lid assembly comprising a chamber, a fill port, a vent between the chamber and an exterior of the transport container, and a valve between the chamber and the sample storage chamber,
wherein the lid assembly is removably coupleable to the sample storage chamber and, when the lid assembly is coupled to the sample storage chamber, the lid assembly is positioned at an inclined angle from a first side to a second side of the storage container and the valve and vent are positioned to direct fluids in the sample chamber to the valve, thereby allowing gases to be purged from the sample chamber through the valve to the chamber and from the chamber through the vent to the exterior of the transport container as the sample chamber is filled with fluid through the fill port, and
wherein the fill port is separate from the adapter and provides a direct fluid path between the exterior of the transport container, through the lid assembly, to the sample storage chamber during operation.
2. The transport container of claim 1 , wherein the chamber is a pumping chamber having a semi-permeable membrane disposed between the pumping chamber and the sample storage chamber at an inclined angle with respect to a horizontal axis when the transport container is placed on a horizontal surface.
3. The transport container of claim 2 , wherein the semi-permeable membrane is inclined at an angle between approximately 1°-30° with respect to the horizontal axis.
4. The transport container of claim 2 , wherein the valve is a ball check valve allows fluid flow from the sample storage chamber to the pumping chamber.
5. The transport container of claim 2 , further comprising a source of oxygen-containing gas.
6. The transport container of claim 5 , wherein the source of oxygen-containing gas is in fluid communication with a first side of the semi-permeable membrane and configured to provide a force against the semi-permeable membrane, thereby causing a second side of the semi-permeable membrane to push against a fluid in the sample storage chamber and to cause the fluid to circulate inside the transport container.
7. The transport container of claim 5 , wherein the source of oxygen-containing gas is a compressed gas cylinder.
8. The transport container of claim 2 , wherein the adapter couples the biological sample to the pumping chamber.
9. The transport container of claim 1 , further comprising a temperature sensor, a pressure sensor, or an oxygen sensor.
10. The transport container of claim 1 further comprising a sensor configured to determine oxygen consumption rate for the biological sample.
11. The transport container of claim 1 wherein one or more of the sample storage chamber and the lid assembly is insulated.
12. The transport container of claim 2 , wherein the semi-permeable membrane is planar.
13. The transport container of claim 1 , wherein the fill port is proximal to a side of the storage container.