Bone-targeting antibodies
Provided are recombinant and chemically-conjugated antibodies and fragments thereof modified with one or more poly-aspartate (poly-D) peptides (e.g., a D10 sequence) to improve localization of the antibodies or fragments to bone. Methods of making and using of these antibodies and fragments also are disclosed.
1. An IgG 4 antibody that binds human TGFβ1, TGFβ2, and TGFβ3, wherein the heavy chain of the antibody comprises the amino acid sequence of SEQ ID NO: 14, and the light chain comprises the amino acid sequence of SEQ ID NO: 15.
2. An IgG 4 antibody that binds human TGFβ1, TGFβ2, and TGFβ3, wherein the heavy chain of the antibody comprises the amino acid sequence of SEQ ID NO: 17, and the light chain comprises the amino acid sequence of SEQ ID NO: 15.
3. A pharmaceutical composition comprising an antibody of claim 1 and a pharmaceutically acceptable excipient.
4. A pharmaceutical composition comprising an antibody of claim 2 and a pharmaceutically acceptable excipient.
5. A method of producing a bone-targeting pharmaceutical composition, comprising:
providing an antibody of claim 1 , and
admixing the antibody with a pharmaceutically acceptable carrier.
6. A method of producing a bone-targeting pharmaceutical composition, comprising:
providing an antibody of claim 2 , and
admixing the antibody with a pharmaceutically acceptable carrier.