Method of detecting gas-producing microbial colonies
A first method comprises using an imaging device to produce a plurality of images of a culture device, analyzing a first image to identify a microorganism colony at a first location, analyzing a second image to identify a gas bubble at a second location, and determining whether the first location is proximate the second location. A second method comprises analyzing an image of a culture device to detect gas bubbles and classifying the gas bubbles according to a size parameter associated with each of the gas bubbles. A third method comprises analyzing a first area of an image of a culture device to detect a first number of gas bubbles, analyzing a second area of the image to detect a second number of gas bubbles, and comparing the first number of gas bubbles to the second number of gas bubbles.
1. A method, comprising:
analyzing an image of the growth area of a thin film culture device to detect gas bubbles; and
classifying a plurality of the gas bubbles, wherein classifying the plurality of gas bubbles comprises assigning each gas bubble to a first group or a second group according to a size parameter associated with each of the plurality of gas bubbles.
2. The method of claim 1 , wherein classifying the gas bubbles into a first group and a second group comprises classifying a first subset of gas bubbles into a first suspect abiogenic bubble group, wherein the method further comprises assigning a size parameter value upper limit for the first suspect abiogenic bubble group.
3. The method of claim 1 , wherein classifying the gas bubbles into a first group and a second group comprises classifying a second subset of gas bubbles into a suspect biogenic bubble group, wherein the method further comprises assigning a size parameter value lower limit for the suspect biogenic bubble group.
4. The method of claim 1 , wherein the thin-film culture medium contains an indicator compound.
5. The method of claim 1 , wherein the thin-film culture medium contains two or more indicator compounds.
6. The method of claim 1 , wherein the thin-film culture medium contains two or more indicator compounds.
7. A non-transitory computer readable medium comprising computer readable instructions that, when executed by a processor, cause an image-analyzing system comprising the processor to:
analyze an image of the growth area of a thin film culture device to detect gas bubbles; and
classify a plurality of the gas bubbles, wherein classifying the plurality of gas bubbles comprises assigning each gas bubble to a first group or a second group according to a size parameter associated with each of the plurality of gas bubbles.
8. The non-transitory computer readable medium of claim 7 , wherein classifying the gas bubbles into a first group and a second group comprises classifying a first subset of gas bubbles into a first suspect abiogenic bubble group, wherein the method further comprises assigning a size parameter value upper limit for the first suspect abiogenic bubble group.
9. The non-transitory computer readable medium of claim 7 , wherein classifying the gas bubbles into a first group and a second group comprises classifying a second subset of gas bubbles into a suspect biogenic bubble group, wherein the method further comprises assigning a size parameter value lower limit for the suspect biogenic bubble group.
10. A method, comprising:
analyzing a first area of an image of a growth area of a thin film culture device to detect a first number of gas bubbles in the first area;
analyzing a second area of the image to detect a second number of gas bubbles in the second area;
comparing the first number of gas bubbles to the second number of gas bubbles;
analyzing the image to detect gas bubbles in the growth area of the culture device; and
classifying a plurality of the gas bubbles, wherein classifying the plurality of gas bubbles comprises assigning each gas bubble to a first group or a second group according to a size parameter associated with each of the plurality of gas bubbles.
11. The method of claim 10 , further comprising:
analyzing a third area of the image to detect a third number of gas bubbles in the third area; and
comparing the first number of gas bubbles or second number of gas bubbles to the third number of gas bubbles.
12. The method of claim 10 , further comprising:
determining whether a gas bubble in any of the first, second, or third areas is assigned to the first group or the second group.
13. The method of claim 10 , wherein the thin-film culture medium contains an indicator compound.
14. A non-transitory computer readable medium comprising computer readable instructions that, when executed by a processor, cause an image-analyzing system comprising the processor to:
analyze a first area of an image of a growth area of a thin film culture device to detect a first number of gas bubbles in the first area;
analyze a second area of the image to detect a second number of gas bubbles in the second area; and
compare the first number of gas bubbles to the second number of gas bubbles;
analyze the image to detect gas bubbles in the growth area of the culture device; and
classifying a plurality of the gas bubbles, wherein classifying the plurality of gas bubbles comprises assigning each gas bubble to a first group or a second group according to a size parameter associated with each of the plurality of gas bubbles.
15. The non-transitory computer readable medium of claim 14 , wherein the computer readable instructions, when executed by a processor, the processor to further:
analyze a third area of the image to detect a third number of gas bubbles in the third area; and
comparing the first number of gas bubbles or second number of gas bubbles to the third number of gas bubbles.