Factor IX polypeptides and methods of use thereof
The present invention provides methods of administering Factor IX; methods of administering chimeric and hybrid polypeptides comprising Factor IX; polynucleotides encoding such chimeric and hybrid polypeptides; cells comprising such polynucleotides; and methods of producing such chimeric and hybrid polypeptides using such cells.
1. A method of treating hemophilia B, comprising intravenously administering to a human subject in need thereof multiple doses of about 50 IU/kg to about 100 IU/kg of a chimeric Factor IX (FIX) polypeptide comprising human FIX having an amino acid sequence identical to amino acids 1 to 415 of SEQ ID NO:2 and a FcRn binding partner (FcRn BP) at a dosing interval of 7 days between two doses, wherein the FcRn BP is human albumin, and wherein the administration treats the human subject by reducing the frequency of spontaneous bleeding.
2. The method of claim 1 , wherein each of the multiple doses is 50 IU/kg to 75 IU/kg.
3. The method of claim 1 , wherein each of the multiple doses is 50 IU/kg.
4. The method of claim 1 , wherein each of the multiple doses is 55 IU/kg.
5. The method of claim 1 , wherein each of the multiple doses is 60 IU/kg.
6. The method of claim 1 , wherein each of the multiple doses is 65 IU/kg.
7. The method of claim 1 , wherein each of the multiple doses is 70 IU/kg.
8. The method of claim 1 , wherein each of the multiple doses is 75 IU/kg.
9. The method of claim 1 , wherein the chimeric polypeptide further comprises a linker joining the FIX and the human albumin.
10. The method of claim 9 , wherein the linker joins the FIX to the N-terminal end of the human albumin.
11. The method of claim 1 , wherein the subject exhibits plasma FIX activity above 1 IU/dL during the dosing interval as measured by a one stage clotting assay that determines activated partial thromboplastin time.
12. The method of claim 11 , wherein each of the multiple doses is 50 IU/kg to 75 IU/kg.
13. The method of claim 11 , wherein each of the multiple doses is 50 IU/kg.
14. The method of claim 11 , wherein each of the multiple doses is 55 IU/kg.
15. The method of claim 11 , wherein each of the multiple doses is 60 IU/kg.
16. The method of claim 11 , wherein each of the multiple doses is 65 IU/kg.
17. The method of claim 11 , wherein each of the multiple doses is 70 IU/kg.
18. The method of claim 11 , wherein each of the multiple doses is 75 IU/kg.
19. The method of claim 11 , wherein the chimeric polypeptide further comprises a linker joining the FIX and the human albumin.
20. The method of claim 19 , wherein the linker joins the FIX to the N-terminal end of the human albumin.