Activation of bioluminescence by structural complementation
View Patent ↗Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.
1. An isolated composition comprising:
(a) a polypeptide comprising SEQ ID NO: 1270;
(b) a first peptide comprising SEQ ID NO: 2587; and
(c) a second peptide comprising SEQ ID NO: 374.
2. The isolated composition of claim 1 , wherein one or more of the polypeptide, first peptide, and second peptide are conjugated to a molecular entity.
3. The isolate composition of claim 2 , wherein the molecular entity is selected from the group consisting of a small molecule, antibody, antibody fragment, peptide, carbohydrate, or protein.