Crispr-CAS10 systems and methods for phage genome editing

1. A phage genome editing system comprising:

a Staphylococcus bacterial cell that can be infected by a phage;

a vector comprising a crRNA that can hybridize to a nucleic acid sequence of the phage; and

a vector comprising a donor nucleic acid sequence, wherein the donor nucleic acid sequence comprises: a mutated nucleic acid sequence to be introduced into the phage, flanked by two nucleic acid sequences containing regions of homology to the phage genome.

2. The system of claim 1 , wherein the Staphylococcus bacterial cell is selected from Staphylococcus epidermidis or Staphylococcus aureus.

3. The system of claim 1 , wherein the Staphylococcus bacterial cell comprises an endogenous CRISPR-Cas10 system.

4. The system of claim 1 , wherein the Staphylococcus bacterial cell has endogenous CRISPR sequences deleted.

5. The system of claim 1 , wherein the phage is a lytic phage.

6. The system of claim 5 , wherein the lytic phage is selected from the group consisting of a Podoviridae phage, a Myoviridae phage, and a Siphoviridae phage.

7. The system of claim 1 , wherein the crRNA and the donor nucleic acid sequence are comprised on the same vector.

8. The system of claim 1 , wherein the mutated nucleic acid sequence is selected from the group consisting of: at least one point mutation, an insertion mutation, and a deletion mutation.