System and method for multi-axis imaging of specimens
A specimen holding and positioning apparatus operable to substantially non-movably maintain a specimen (e.g., an excised tissue specimen) in a fixed or stable orientation with respect to the apparatus during imaging operations (e.g., x-ray imaging), transport (e.g., from a surgery room to a pathologist's laboratory), and the like for use in facilitating accurate detection and diagnosis of cancers and/or other abnormalities of the specimen.
1. A method of imaging a specimen, comprising:
elastically deforming first and second retention members of a positioning apparatus to surround opposing portions of a specimen;
securing the first and second retention members against movement relative to each other to retain the specimen therebetween, wherein a reference plane extends between the first and second retention members when the specimen is retained therebetween;
generating, at a source of electromagnetic radiation, a beam of electromagnetic radiation;
sending, while the specimen is retained between the first and second retention members, the beam along an imaging axis through the specimen and the first and second retention members; and
receiving the beam at an imaging detector, wherein the beam is free of contact with any structures other than the specimen and the first and second retention members along the imaging axis between the source and the imaging detector.
2. The method of claim 1 , further including before the elastically deforming:
disposing the specimen within an opening of the first retention member.
3. The method of claim 2 , wherein the elastically deforming includes:
inserting the second retention member into the opening of the first retention member.
4. The method of claim 1 , wherein the imaging axis is perpendicular to the reference plane.
5. The method of claim 1 , further comprising after the receiving:
reorienting the reference plane relative to the imaging axis;
second generating a beam of electromagnetic radiation;
second sending the beam along the imaging axis through the specimen and the first and second retention members; and
second receiving the beam at the imaging sensor, wherein the beam is free of contact with any structures other than the specimen and the first and second retention members along the imaging axis between the source and the imaging detector.
6. The method of claim 5 , wherein the imaging axis is perpendicular to the reference plane during the sending, and wherein the imaging axis is substantially parallel to the reference plane during the second sending.
7. The method of claim 5 , wherein the reorienting includes reorienting the positioning apparatus by 90 degrees.
8. The method of claim 5 , further comprising:
generating a first image of the specimen from the receiving;
generating a second image of the specimen from the second receiving; and
using the first and second images to verify tissue margins of the specimen.
9. The method of claim 1 , further comprising:
separating the first and second retention members from each other;
lifting the specimen away from the first retention member;
locating a location identification member on the first retention member;
placing the specimen over the location identification member;
advancing at least one of the first and second retention members towards the other of the first and second retention members;
elastically deforming, during the advancing, the first and second retention members about the location identification member and the specimen;
securing, after the elastically deforming, the first and second retention members against movement relative to each other to retain the specimen and location identification member between the first and second retention members;
second generating a beam of electromagnetic radiation;
second sending the beam along the imaging axis through the specimen, the first and second retention members, and the location identification member; and
second receiving the beam at the imaging detector, wherein the beam is free of contact with any structures other than the specimen, the first and second retention members, and the location identification member along the imaging axis between the source and the imaging detector.
10. The method of claim 9 , wherein the location identification member comprises radiopaque indicia across at least a surface thereof, wherein an image generated from the second receiving includes the indicia, and wherein the method further includes:
using the indicia of the image to identify one or more areas of interest in the specimen.
11. The method of claim 1 , wherein a total attenuation of the beam through the first and second retention members along the imaging axis is less than a total attenuation of the beam through the specimen along the imaging axis.