System and process for purification of astatine-211 from target materials
A new column-based purification system and approach are described for rapid separation and purification of the alpha-emitting therapeutic radioisotope 211 At from dissolved cyclotron targets that provide highly reproducible product results with excellent 211 At species distributions and high antibody labeling yields compared with prior art manual extraction results of the prior art that can be expected to enable enhanced production of purified 211 At isotope products suitable for therapeutic medical applications such as treatment of cancer in human patients.
1. A method for producing a purified 211 At isotope product from dissolved cyclotron targets, the method comprising:
dissolving a cyclotron target containing 211 At and Bi in a nitric acid solution to form a first solution comprising 211 At and Bi-nitrate salts;
removing the liquid from the first solution to form a first composition comprising 211 At and Bi-nitrate salts;
dissolving the first composition in an HCl solution to form a second solution comprising the 211 At and Bi in an HCl solution;
preparing a separation media with conditioning solution comprising HCl to acidify the separation media;
passing the second solution through the separation media to associate 211 At isotopes with the media and recover Bi as an eluent; and
passing an At elution solution comprising HNO 3 through the separation media to elute 211 At isotopes from the separation media and provide 211 At isotopes in an HNO 3 solution.
2. The method of claim 1 further comprising the step of washing the column with a wash solution comprising HCl prior to passing the At elution solution through the separation media to elute 211 At isotopes.
3. The method of claim 1 wherein the At elution solution has a molarity between 7.5 M and 15.8 M.
4. The method of claim 1 wherein the cyclotron target is a bismuth target.
5. The method of claim 1 wherein the separation media is a non-PEG media.
6. The method of claim 5 wherein the separation media is a macroporous polymer resin.
7. The method of claim 1 wherein the conditioning solution is 8 M HCl.
8. The method of claim 7 further comprising passing the conditioning solution through the separation media for at least one sample column at a flow rate less than or equal to 2 ml/min.
9. The method of claim 1 further comprising providing a first volume of the second solution to the separation media and providing a second volume of the At elution solution to the separation media, wherein the first volume is greater than the second volume.
10. The method of claim 9 wherein the second solution is provided to the separation media at a flow rate less than or equal to 2 mL/min.