HPPD-inhibitor herbicide tolerance
This invention relates generally to the detection of genetic differences among soybeans. More particularly, the invention relates to soybean quantitative trait loci (QTL) for tolerance or sensitivity to HPPD-inhibitor herbicides, such as mesotrione and isoxazole herbicides, to soybean plants possessing these QTLs, which map to a novel chromosomal region, and to genetic markers that are indicative of phenotypes associated with tolerance, improved tolerance, susceptibility, or increased susceptibility. Methods and compositions for use of these markers in genotyping of soybean and selection are also disclosed, as are methods and compositions for use of these markers in selection and use of herbicides for weed control. Also disclosed are isolated polynucleotides and polypeptides relating to such tolerance or sensitivity and methods of introgres sing such tolerance into a plant by breeding or transgenically or by a combination thereof. Plant cells, plants, and seeds produced are also provided.
1. A method for selectively screening soybean plants for tolerance to one or more herbicides selected from the group consisting of a mesotrione herbicide and an isoxazole herbicide, the method comprising:
(a) crossing a first soybean plant with a second soybean plant to form a soybean plant or soybean germ plasm population;
(b) isolating nucleic acids from the soybean plant or soybean germplasm of the population;
(c) detecting in the nucleic acids at least one allele of one or more marker loci associated with tolerance or improved tolerance to the one or more herbicides, wherein the one or more marker loci localize within a chromosome interval selected from the group consisting of:
(i) the chromosomal interval flanked by and including markers S04867-1-A and S03859-1-A on linkage group L
(ii) the chromosomal interval flanked by and including markers S08110-1-Q1 and S08010-1-Q1 on linkage group L;
(iii) the chromosomal interval flanked by and including markers S08117-1-Q1 and S08010-1-Q1 on linkage group L;
(iv) the chromosomal interval flanked by and including markers S08110-1-Q1 and S08105-1-Q1 on linkage group L;
(v) the chromosomal interval flanked by and including markers S08117-1-Q1 and S08105-1-Q1 on linkage group L; and
(vi) the chromosomal interval flanked by and including markers S08113-1-Q1 and S08105-1-Q1 on linkage group L;
(d) selecting the soybean plant or soybean germplasm comprising the at least one allele of one or more marker loci thereby selecting a plant with tolerance or improved tolerance to the one or more herbicides.
2. The method of claim 1 , wherein the one or more marker loci are selected from the group consisting of SATT723, Sat_408, A169_1, EV2_1, Sle3_4s, BLT010_2, BLT007_1, SATT232, S04867-1-A, S08102-1-Q1, S08103-1-Q1, S08104-1-Q1, S08106-1-Q1, S08107-1-Q1, S08109-1-Q1, S08110-1-Q1, SO8111-1-Q1, S08115-2-Q1, S08117-1-Q1, S08119-1-Q1, S08116-1-Q1, S08112-1-Q1, S08108-1-Q1, S08101-4-Q1, S08101-1-Q1, S08101-2-Q1, S08101-3-Q1, SO8118-1-Q1, S08114-1-Q1, S08113-1-Q1, S03859-1-A, Sat_301, SATT446, SATT232, S08105-1-Q1, S08010-1-Q1, S08010-2-Q1, R176_1, JUBC090, SATT238, Sat_071, BLT039_1, Bng071_1, A264_1, RGA_7, RGA7, SATT523, Sat_134, S00224-1, S01659-1, LbA, i8_2, A450_2, A106_1, Sat_405, SATT143, B124_2, A459_1, SATT398, SATT694, Sat_195, Sat_388, SATT652, SATT711, Sat_187, SATT418, SATT278, Sat_397, Sat_191, Sat_320, 0109_1, A204_2, SATT497, G214_17, B164_1, G214_16, A023_1, SATT284, AW508247, SATT462, L050_7, E014_1, A071_5, B046_1, L1, and B162_2.
3. The method of claim 1 , wherein the screening occurs as part of further breeding to improve a soybean variety's tolerance to one or more mesotrione herbicides or one or more isoxazole herbicides, and wherein the further breeding comprises crosses with other lines, crosses with hybrids, backcrossing, selfcrossing, or combinations thereof.