Porcine epidemic diarrhea virus vaccine
The present invention relates to immunogenic compositions, wherein the immunogenic compositions include a recombinant spike antigen porcine epidemic diarrhea virus (PEDV). The immunogenic compositions commonly include an oil-in-water emulsion as an adjuvant. Methods for producing the immunogenic compositions and the recombinant PEDV spike antigen are also provided.
1. An immunogenic composition comprising a recombinant spike antigen of porcine epidemic diarrhea virus (PEDV) having a heterologous C-terminal domain and a heterologous transmembrane domain, and an oil-in-water emulsion as an adjuvant, wherein the porcine epidemic diarrhea virus (PEDV) is a PEDV of North American origin and
a. wherein the spike antigen is encoded by a nucleic acid sequence that is at least 95% identical with SEQ ID NO: 8 over the length of SEQ ID NO: 8.
2. The immunogenic composition of claim 1 , wherein the recombinant antigen comprises one or more immunogenic components selected from the group consisting of:
a. an isolated nucleic acid encoding an antigen of porcine epidemic diarrhea virus (PEDV) spike protein, wherein the antigen has at least 95% homology with SEQ ID NO:3, 7, 9 or 14,
b. a recombinant vector comprising the isolated nucleic acid of a),
c. the recombinant PEDV Spike protein encoded by the nucleic acid of a), and
d. any combination thereof.
3. The immunogenic composition of claim 2 , wherein such immunogenic composition comprises a pharmaceutical acceptable carrier and/or an excipient.
4. The immunogenic composition of claim 1 , wherein the oil-in-water emulsion is an EMULSIGEN® oil-in-water emulsion-based adjuvant.
5. The immunogenic composition of claim 2 , wherein an immunogenic component is the isolated nucleic acid.
6. The immunogenic composition of claim 2 , wherein an immunogenic component is the recombinant vector.
7. The immunogenic composition of claim 2 , wherein an immunogenic component is the recombinant porcine epidemic diarrhea virus (PEDV) Spike protein.
8. The immunogenic composition of claim 2 , wherein an immunogenic component is a combination.
9. The immunogenic composition of claim 2 , further comprising at least one additional antigen associated with a pathogen other than porcine epidemic diarrhea virus.
10. A kit for inducing an immunogenic response in a pig against porcine epidemic diarrhea virus (PEDV) comprising:
a. a dispenser capable of administering an immunogenic composition to a pig; and
b. the immunogenic composition according to claim 2 .
11. A method of producing an immunogenic composition comprising:
a. expressing an antigen of porcine epidemic diarrhea virus (PEDV) comprising the recombinant antigen of claim 2 in a host cell,
b. harvesting the antigen of porcine epidemic diarrhea virus (PEDV) cells, and
c. adding an oil-in-water emulsion-based adjuvant to the porcine epidemic diarrhea virus (PEDV) antigen of step b).
12. The method of claim 11 , wherein the PEDV antigen comprises:
a. an isolated nucleic acid encoding an antigen of porcine epidemic diarrhea virus (PEDV) spike protein, wherein the recombinant Spike polypeptide has at least 95% homology with SEQ ID NO:3, 7, 9 or 14,
b. a recombinant vector comprising the isolated nucleic acid of a),
c. the recombinant PEDV Spike protein encoded by the nucleic acid of a), and
d. any combination thereof.
13. The method of claim 11 , wherein the antigen of porcine epidemic diarrhea virus (PEDV) is expressed by a recombinant baculovirus vector.
14. The method of claim 13 , wherein the antigen of porcine epidemic diarrhea virus (PEDV) is expressed in insect cells.
15. The method of claim 11 , wherein the oil-in-water emulsion-based adjuvant is an EMULSIGEN® oil-in-water emulsion-based adjuvant.
16. A method for protecting a pig against diseases associated with porcine epidemic diarrhea virus (PEDV), comprising administering to such pig the vaccine of claim 1 .