Device and methods for quantifying analytes
The present invention relates to devices and methods for measuring the quantity of multiple analytes in a sample. The device is designed such that each of the analyte sensing elements is configured to measure the quantity of a predetermined analyte and where the machine executable instructions are configured to select the proper analyte sensing element corresponding to the analyte to be measured.
1. A method of calculating a ratio of a first analyte to a second analyte in a sample with an instrument, the method comprising:
receiving a selection of an assay from an input device of the instrument;
measuring a first fluorescence intensity associated with a first label of a first analyte and a second fluorescence intensity associated with a second label of a second analyte in the sample, wherein measuring the first and second fluorescence intensities includes exciting the first label with a first energy source and exciting the second label with a second energy source, wherein the first and second energy sources emit different wavelengths of electromagnetic energy, and wherein the first and second energy sources are automatically selected based upon the selection of the assay received from the input device by a processor using machine executable instructions; and
calculating the ratio of the first analyte to the second analyte based upon the first fluorescence intensity and the second fluorescence intensity.
2. The method of claim 1 , wherein the selection of the assay from the input device occurs through a user interface.
3. The method of claim 1 , wherein the first and second analytes are independently selected from the group consisting of DNA, RNA, proteins, carbohydrates, lipids, proteoglycans, glycoproteins, proteolipids, lipoproteins, metal ions, prokaryotic cells, eukaryotic cells, and viral particles.
4. The method of claim 1 , wherein the first and second fluorescence intensities are measured with one or more photodetectors.
5. The method of claim 1 , wherein the first and second fluorescence intensities are measured simultaneously.
6. The method of claim 1 , wherein the first energy source emits electromagnetic energy comprising blue light and the second energy source emits electromagnetic energy comprising red light.
7. The method of claim 1 , wherein the first and second labels are fluorophores with different spectral properties.
8. The method of claim 1 , wherein the instrument additionally comprises one or more emission filters, and wherein the first and second fluorescence intensities are measured using the one or more emission filters.
9. The method of claim 1 , wherein the instrument comprises one or more excitation filters associated with each of the one or more energy sources.
10. The method of claim 1 , wherein the instrument comprises one or more emission filters associated with each of the one or more energy sources.
11. The method of claim 1 , wherein the at least two different energy sources are part of at least two different analyte sensing elements, wherein each analyte sensing element comprises one of the at least two different energy sources, an excitation filter and an emission filter.
12. The method of claim 1 , wherein calculating the ratio of the first analyte to the second analyte is additionally based upon one or more standard curves.
13. The method of claim 12 , additionally comprising:
measuring a fluorescence intensity associated with a standard, wherein measuring the fluorescence intensity associated with the standard includes exciting the standard with the first or second energy source, and wherein the first or second energy source is automatically selected based upon the selection of the assay received from the input device by the processor.
14. The method of claim 12 , wherein the one or more standard curves are based upon (i) one or more samples without the first and second analytes and (ii) one or more samples with a known concentration of the first analyte, the second analyte, or the first and second analytes.
15. The method of claim 1 , wherein calculating the ratio of the first analyte to the second analyte additionally comprises:
quantitating the first and second analytes based upon the first and second fluorescence intensities.
16. The method of claim 1 , additionally comprising:
displaying the ratio of the first analyte to the second analyte through a user interface.
17. The method of claim 1 , additional comprising:
storing the ratio of the first analyte to the second analyte in a memory storage device.