IP Library › Granted Patent US 11,066,666
Granted Patent B2
US 11,066,666 · App. 15/925,172 · Granted Jul 20, 2021

Bioactive renal cells

Inventors: Timothy A. Bertram (George Town, KY); Roger M. Ilagan (Burlington, NC); Russell W. Kelley (Winston-Salem, NC); Sharon C. Presnell (Lewisville, NC); Sumana Choudhury (Kernersville, NC); Andrew T. Bruce (Lexington, NC); Christopher W. Genheimer (Colfax, NC); Bryan R. Cox (Winston-Salem, NC); Kelly I. Guthrie (Winston-Salem, NC); Joydeep Basu (Winston-Salem, NC); Shay M. Wallace (Winston-Salem, NC); Eric Werdin (Lewisville, TX); Oluwatoyin A. Knight (Winston-Salem, NC); Namrata D. Sangha (Winston-Salem, NC); John W. Ludlow (Carrboro, NC); Craig R. Halberstadt (Clemmons, NC); Richard Payne (Winston-Salem, NC); Neil F. Robins (Winston-Salem, NC); Darell McCoy (Clemmons, NC); Deepak Jain (Winston-Salem, NC); Manuel J. Jayo (Winston-Salem, NC); Elias A. Rivera (Oak Ridge, NC); Thomas Spencer (Winston-Salem, NC); Benjamin Watts (King, NC)
Assignee: inRegen
C12N15/113C12N5/0686C12Q1/6883C12N2310/141C12N2320/30C12N2320/32C12Q2600/106C12Q2600/178
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Quick Facts
Patent No.
US 11,066,666
App. No.
15/925,172
Granted
Jul 20, 2021
Kind
B2
Abstract

The present invention concerns bioactive renal cells populations, renal cell constructs, and methods of making and using the same.

Claims (20)

1. A method of providing a regenerative effect to a native kidney comprising in vivo contacting the native kidney with a composition comprising isolated human secreted vesicles produced by a renal cell population enriched for bioactive kidney cells,

wherein the vesicles comprise exosomes or microvesicles comprising a paracrine factor that attenuates Plasminogen Activation Inhibitor-1 (PAI-1) and/or Transforming Growth Factor Beta (TGFβ) signaling,

wherein the vesicles have been isolated from the renal cell population, and

wherein the regenerative effect comprises a reduction in renal fibrosis.

2. The method of claim 1 , wherein the vesicles comprise microvesicles.

3. The method of claim 1 , wherein the vesicles comprise exosomes.

4. The method of claim 1 , wherein the paracrine factor inhibits PAI-1 signaling.

5. The method of claim 1 , wherein the paracrine factor is an miRNA.

6. The method of claim 5 , wherein the miRNA inhibits PAI-1 signaling.

7. The method of claim 1 , wherein the paracrine factor inhibits TGFβ.

8. The method of claim 7 , wherein the paracrine factor is an miRNA that inhibits TGFβ.

9. The method of claim 1 , wherein renal fibrosis is reduced by inhibition of epithelial-to-mesenchymal transition (EMT).

10. The method of claim 1 , wherein the bioactive kidney cells comprise:

(a) tubular cells; or

(b) tubular cells and one or more of glomerular cells and vascular cells.

11. The method of claim 10 , wherein the bioactive cells comprise tubular cells and glomerular cells.

12. The method of claim 10 , wherein the bioactive cells comprise tubular cells and vascular cells.

13. The method of claim 1 , wherein the renal cell population is non-autologous to the native kidney.

14. The method of claim 1 , wherein the renal cell population is autologous to the native kidney.

15. The method of claim 6 , wherein the miRNA comprises microRNA 30b-5p or microRNA 449a.

Assignments (7)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 3, 2024
From: BERTRAM, TIMOTHY A.
To: INREGEN
Reel/Frame 067601/0467 →
MERGER Recorded Feb 28, 2023
From: REGENMEDTX, LLC
To: REGENMED (CAYMAN) LTD.
Reel/Frame 062826/0592 →
CHANGE OF NAME Recorded Feb 28, 2023
From: INREGEN
To: PROKIDNEY
Reel/Frame 062886/0426 →
CHANGE OF NAME Recorded Feb 28, 2023
From: REGENMED (CAYMAN) LTD.
To: INREGEN
Reel/Frame 062886/0482 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 28, 2023
From: TENGION, INC.
To: REGENMEDTX, LLC
Reel/Frame 062916/0182 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 31, 2021
From: ILAGAN, ROGER M.; KELLEY, RUSSELL W.; PRESNELL, SHARON C.; CHOUDHURY, SUMANA; BRUCE, ANDREW T.; GENHEIMER, CHRISTOPHER W.; COX, BRYAN R.; GUTHRIE, KELLY I.; BASU, JOYDEEP; WALLACE, SHAY M.; WERDIN, ERIC S.; KNIGHT, OLUWATOYIN A.; SANGHA, NAMRATA D.; LUDLOW, JOHN W.; HALBERSTADT, CRAIG R.; PAYNE, RICHARD; ROBBINS, NEIL FRANCES; MCCOY, DARELL; JAIN, DEEPAK; JAYO, MANUEL J.; RIVERA, ELIAS A.; SPENCER, THOMAS; WATTS, BENJAMIN
To: TENGION, INC.
Reel/Frame 057335/0121 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 31, 2021
From: ILAGAN, ROGER M.; KELLEY, RUSSELL W.; PRESNELL, SHARON C.; CHOUDHURY, SUMANA; BRUCE, ANDREW T.; GENHEIMER, CHRISTOPHER W.; COX, BRYAN R.; GUTHRIE, KELLY I.; BASU, JOYDEEP; WALLACE, SHAY M.; WERDIN, ERIC S.; KNIGHT, OLUWATOYIN A.; SANGHA, NAMRATA D.; LUDLOW, JOHN W.; HALBERSTADT, CRAIG R.; PAYNE, RICHARD; ROBINS, NEIL F.; MCCOY, DARELL; JAIN, DEEPAK; JAYO, MANUEL J.; RIVERA, ELIAS A.; SPENCER, THOMAS; WATTS, BENJAMIN
To: TENGION, INC.
Reel/Frame 057335/0827 →
Continuity (12)
Continuation 13697206
Provisional Application 61473111 · Apr 7, 2011
Provisional Application 61441423 · Feb 10, 2011
Provisional Application 61412933 · Nov 12, 2010
Provisional Application 61413382 · Nov 12, 2010
Provisional Application 61388765 · Oct 1, 2010
Provisional Application 61376586 · Aug 24, 2010
Provisional Application 61372077 · Aug 9, 2010
Provisional Application 61371888 · Aug 9, 2010
Provisional Application 61353895 · Jun 11, 2010
Provisional Application 61334032 · May 12, 2010
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