Methods and compositions for selecting soybean plants resistant to phytophthora root rot
The present invention relates to the field of plant breeding and disease resistance. More specifically, the invention includes a method for breeding soybean plants containing quantitative trail loci (QTL) for resistance the Phytophthora root rot (PRR) caused by Phytophthora sojae . The invention further includes the use of molecular markers in the introgression of PRR resistance QTL into soybean plants.
1. A method of introgressing at least one PRR resistance QTL into a soybean plant comprising
a. crossing at least one Phytophthora Root Rot (PRR) resistant soybean plant with at least one second soybean plant to form a population;
b. screening said population with at least one nucleic acid marker to detect a polymorphism genetically linked to PRR resistance;
c. selecting from said population one or more soybean plants comprising a haplotype associated with PRR resistance, wherein the PRR resistance haplotype comprises the polymorphism, and wherein the polymorphism is genetically linked to a locus comprising SEQ ID NO: 7.
2. The method according to claim 1 , wherein at least 51% or at least 75% of the selected soybean plants exhibit a resistant reaction to PRR.
3. The method of claim 1 , further comprising the step (d) of assaying said selected soybean plant for resistance to a PRR inducing pathogen.
4. The method of claim 1 , wherein said genotype is determined by an assay which is selected from the group consisting of single base extension (SBE), allele-specific primer extension sequencing (ASPE), DNA sequencing, RNA sequencing, microarray-based analyses, universal PCR, allele specific extension, hybridization, mass spectrometry, ligation, extension-ligation, and Flap Endonuclease-mediated assays.
5. The method of claim 1 , further comprising the step of crossing the soybean plant selected in step (c) to another soybean plant.
6. The method of claim 1 , further comprising the step of obtaining seed from the soybean plant selected in step (c).
7. A method of introgressing an allele into a soybean plant comprising:
a. crossing a PRR resistant soybean plant with a second soybean plant to form a population of soybean plants,
b. genotyping at least one soybean plant in the population with a soybean genomic nucleic acid marker to detect a polymorphism genetically linked to Phytophthora Root Rot (PRR) resistance, and
c. selecting from said population one or more soybean plants comprising a haplotype associated with PRR resistance, wherein said PRR resistance haplotype comprises the polymorphism and the polymorphism is genetically linked to a locus comprising SEQ ID NO: 7.
8. The method according to claim 7 , wherein said selected one or more soybean plants exhibit increased grain yield in the presence of PRR as compared to soybean plants lacking PRR resistance loci.
9. The method according to claim 8 , wherein said selected one or more soybean plants exhibit an increased grain yield of at least 0.5 Bu/A, at least 1.0 Bu/A or at least 1.5 Bu/A in the presence of PRR as compared to soybean plants lacking PRR resistance loci.