Anti-SARS-CoV-2 antibodies derived from 2DD8
This disclosure provides antibodies and antigen-binding fragments that are derived from 2dd8 and that can be administered to an individual that is infected or suspected of being infected with a virus. Antibodies and antigen-binding fragments herein can be capable of treating or curing the virus, and which may provide protection against the virus for up to several weeks. Antibodies and antigen-binding fragments herein can be used to diagnose a SARS CoV-2 infection.
1. An antibody or an antigen-binding fragment that selectively binds to a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), that comprises a variable heavy chain (VH) complementarity determining region 1 (CDR1) having an amino acid sequence of SEQ ID NO: 70; a VH CDR2 having an amino acid sequence of SEQ ID NO: 116; a VH CDR3 having an amino acid sequence of SEQ ID NO: 165; a variable light chain (VL) CDR1 having an amino acid sequence of SEQ ID NO: 3; a VL CDR2 having an amino acid sequence of SEQ ID NO: 6; and a VL CDR3 having an amino acid sequence of SEQ ID NO: 26.
2. An antibody or an antigen-binding fragment that selectively binds to a SARS-CoV-2, that comprises the CDRs of claim 1 , and that comprises a VH having an amino acid sequence that is at least 90% identical to SEQ ID NO: 207 and a VL having an amino acid sequence that is at least 90% identical to SEQ ID NO: 248.
3. The antibody or the antigen-binding fragment of claim 1 , that selectively binds to a receptor binding domain (RBD) of SARS-CoV-2.
4. The antibody or the antigen-binding fragment of claim 2 , that selectively binds to a receptor binding domain (RBD) of SARS-CoV-2.
5. The antibody or the antigen-binding fragment of claim 1 , wherein the antibody is an IgG, an IgM, an IgE, an IgA, or an IgD, or is derived therefrom.
6. The antibody or the antigen-binding fragment of claim 5 , wherein the antibody is an IgG selected from the group consisting of an IgG1, an IgG2a, an IgG2b, an IgG3, and an IgG4.
7. The antibody or the antigen-binding fragment of claim 2 , wherein the antibody is an IgG, an IgM, an IgE, an IgA, or an IgD, or is derived therefrom.
8. The antibody or the antigen-binding fragment of claim 7 , wherein the antibody is an IgG selected from the group consisting of an IgG1, an IgG2a, an IgG2b, an IgG3, and an IgG4.
9. The antibody or the antigen-binding fragment of claim 1 , wherein the antibody comprises a monoclonal antibody, a grafted antibody, a chimeric antibody, a human antibody, or a humanized antibody.
10. The antibody or the antigen-binding fragment of claim 2 , wherein the antibody comprises a monoclonal antibody, a grafted antibody, a chimeric antibody, a human antibody, or a humanized antibody.
11. The antibody or the antigen-binding fragment of claim 1 , that comprises a binding affinity of less than 50 nM.
12. The antibody or the antigen-binding fragment of claim 2 , that comprises a binding affinity of less than 50 nM.
13. The antibody or the antigen-binding fragment of claim 1 , wherein the antigen-binding fragment comprises a Fab, a Fab′, a F(ab′) 2 , a variable fragment (Fv), a triabody, a tetrabody, a minibody, a bispecific F(ab′) 2 , a trispecific F(ab′) 2 , a diabody, a bispecific diabody, a single chain variable fragment (scFv), a scFv-Fc, a Fab-Fc, a VHH, or a bispecific scFv.
14. The antibody or the antigen-binding fragment of claim 2 , wherein the antigen-binding fragment comprises a Fab, a Fab′, a F(ab′) 2 , a variable fragment (Fv), a triabody, a tetrabody, a minibody, a bispecific F(ab′) 2 , a trispecific F(ab′) 2 , a diabody, a bispecific diabody, a single chain variable fragment (scFv), a scFv-Fc, a Fab-Fc, a VHH, or a bispecific scFv.
15. An antibody or an antigen-binding fragment that selectively binds to a SARS-CoV-2, wherein the antibody or the antigen-binding fragment comprises a VH chain having an amino acid sequence of SEQ ID NO: 207 and a VL chain having an amino acid sequence of SEQ ID NO: 248.
16. An antibody or an antigen-binding fragment that selectively binds to a SARS-CoV-2, that comprises:
(i) a VH CDR1 having an amino acid sequence of SEQ ID NO: 58, a VH CDR2 having an amino acid sequence of SEQ ID NO: 104, a VH CDR3 having an amino acid sequence of SEQ ID NO: 153, a VL CDR1 having an amino acid sequence of SEQ ID NO: 3, a VL CDR2 having an amino acid sequence of SEQ ID NO: 6, and a VL CDR3 having an amino acid sequence of SEQ ID NO: 14;
(ii) a VH CDR1 having an amino acid sequence of SEQ ID NO: 83, a VH CDR2 having an amino acid sequence of SEQ ID NO: 129, a VH CDR3 having an amino acid sequence of SEQ ID NO: 178, a VL CDR1 having an amino acid sequence of SEQ ID NO: 3, a VL CDR2 having an amino acid sequence of SEQ ID NO: 6, and a VL CDR3 having an amino acid sequence of SEQ ID NO: 39;
(iii) a VH CDR1 having an amino acid sequence of SEQ ID NO: 57, a VH CDR2 having an amino acid sequence of SEQ ID NO: 103, a VH CDR3 having an amino acid sequence of SEQ ID NO: 152, a VL CDR1 having an amino acid sequence of SEQ ID NO: 3, a VL CDR2 having an amino acid sequence of SEQ ID NO: 8, and a VL CDR3 having an amino acid sequence of SEQ ID NO: 13; or
(iv) a VH CDR1 having an amino acid sequence of SEQ ID NO: 76, a VH CDR2 having an amino acid sequence of SEQ ID NO: 122, a VH CDR3 having an amino acid sequence of SEQ ID NO: 171, a VL CDR1 having an amino acid sequence of SEQ ID NO: 3, a VL CDR2 having an amino acid sequence of SEQ ID NO: 6, and a VL CDR3 having an amino acid sequence of SEQ ID NO: 32.
17. A method of preventing or treating a SARS-CoV-2 viral infection or COVID19 in a subject in need thereof, comprising administering to the subject the antibody or the antigen-binding fragment of claim 1 .
18. The method of claim 17 , that further comprises administering one or more additional therapies or drugs to the subject.
19. A method of diagnosing a subject as being infected with a SARS-CoV-2 virus or suspected of being infected with a SARS-CoV-2 virus, the method comprising contacting a sample obtained from the subject with the antibody or the antigen-binding fragment of claim 1 ; detecting the presence or absence of the antibody or the antigen-binding fragment; and diagnosing the subject as being infected with a SARS-CoV-2 virus when the presence of the antibody or the antigen-binding fragment is detected.
20. The method of claim 19 , wherein the sample comprises a nasal swab, a tissue sample, saliva, or blood.
21. The method of claim 19 , wherein detecting the presence or absence of the antibody or the antigen-binding fragment comprises an enzyme linked immunosorbent assay (ELISA), an immunospot assay, a lateral flow assay, flow cytometry, immunohistochemistry, or a western blot.