Anti-SARS-Cov-2 antibodies derived from 6nb6
This disclosure provides antibodies and antigen-binding fragments that are derived from 6nb6 and that can be administered to an individual that is infected or suspected of being infected with a virus. Antibodies and antigen-binding fragments herein can be capable of treating or curing the virus, and which may provide protection against the virus for up to several weeks. Antibodies and antigen-binding fragments herein can be used to diagnose a SARS CoV 2 infection.
1. An antibody or an antigen-binding fragment that selectively binds to a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), that comprises a variable heavy chain (VH) complementarity determining region 1 (CDR1) having an amino acid sequence of SEQ ID NO: 136; a VH CDR2 having an amino acid sequence of SEQ ID NO: 190; a VH CDR3 having an amino acid sequence of SEQ ID NO: 223; a variable light chain (VL) CDR1 having an amino acid sequence of SEQ ID NO: 8; a VL CDR2 having an amino acid sequence of SEQ ID NO: 38; and a VL CDR3 having an amino acid sequence of SEQ ID NO: 120.
2. A method of preventing or treating a SARS-CoV-2 viral infection or COVID19 in a subject in need thereof, comprising administering to the subject the antibody or the antigen-binding fragment of claim 1 .
3. The method of claim 2 , that further comprises administering one or more additional therapies or drugs to the subject.
4. A method of diagnosing a subject as being infected with a SARS-Cov-2 virus or suspected of being infected with a SARS-Cov-2 virus, the method comprising contacting a sample obtained from the subject with the antibody or the antigen-binding fragment of claim 1 ; detecting the presence or absence of the antibody or the antigen-binding fragment; and diagnosing the subject as being infected with a SARS-CoV-2 virus when the presence of the antibody or the antigen-binding fragment is detected.
5. The method of claim 4 , wherein the sample comprises a nasal swab, a tissue sample, saliva, or blood.
6. The method of claim 4 , wherein detecting the presence or absence of the antibody or the antigen-binding fragment comprises an enzyme linked immunosorbent assay (ELISA), an immunospot assay, a lateral flow assay, flow cytometry, immunohistochemistry, or a western blot.
7. An antibody or an antigen-binding fragment that selectively binds to a SARS-CoV-2, that comprises the CDRs of claim 1 , and that comprises a VH having an amino acid sequence that is at least 90% identical to SEQ ID NO: 428 and a VL having an amino acid sequence that is at least 90% identical to SEQ ID NO: 490.
8. The antibody or the antigen-binding fragment of claim 7 , that selectively binds to a receptor binding domain (RBD) of SARS-CoV-2.
9. The antibody or the antigen-binding fragment of claim 7 , wherein the antibody comprises a monoclonal antibody, a grafted antibody, a chimeric antibody, a human antibody, or a humanized antibody.
10. The antibody or the antigen-binding fragment of claim 7 , that comprises a binding affinity of less than 50 nM.
11. The antibody or the antigen-binding fragment of claim 7 , wherein the antigen-binding fragment comprises a Fab, a Fab′, a F(ab′)2, a variable fragment (Fv), a triabody, a tetrabody, a minibody, a bispecific F(ab′)2, a trispecific F(ab′)2, a diabody, a bispecific diabody, a single chain variable fragment (scFv), a scFv-Fc, a Fab-Fc, a VHH, or a bispecific scFv.
12. The antibody or the antigen-binding fragment of claim 7 wherein the antibody is an IgG, an IgM, an IgE, an IgA, an IgD, or is derived therefrom.
13. The antibody or the antigen-binding fragment of claim 12 , wherein the antibody is an IgG selected from the group consisting of an IgG1, an IgG2a, an IgG2b, an IgG3, and an IgG4.
14. The antibody or the antigen-binding fragment of claim 1 , that selectively binds to a receptor binding domain (RBD) of SARS-CoV-2.
15. The antibody or the antigen-binding fragment of claim 1 , that comprises a binding affinity of less than 50 nM.
16. The antibody or the antigen-binding fragment of claim 1 , wherein the antibody comprises a monoclonal antibody, a grafted antibody, a chimeric antibody, a human antibody, or a humanized antibody.
17. The antibody or the antigen-binding fragment of claim 1 , wherein the antigen-binding fragment comprises a Fab, a Fab′, a F(ab′)2, a variable fragment (Fv), a triabody, a tetrabody, a minibody, a bispecific F(ab′)2, a trispecific F(ab′)2, a diabody, a bispecific diabody, a single chain variable fragment (scFv), a scFv-Fc, a Fab-Fc, a VHH, or a bispecific scFv.
18. The antibody or the antigen-binding fragment of claim 1 , wherein the antibody is an IgG, an IgM, an IgE, an IgA, an IgD, or is derived therefrom.
19. The antibody or the antigen-binding fragment of claim 18 , wherein the antibody is an IgG selected from the group consisting of an IgG1, an IgG2a, an IgG2b, an IgG3, and an IgG4.
20. An antibody or an antigen-binding fragment that selectively binds to a SARS-CoV-2, that comprises a VH chain having an amino acid sequence of SEQ ID NO: 428 and a VL chain having an amino acid sequence of SEQ ID NO: 490.
21. An antibody or an antigen-binding fragment that selectively binds to a SARS-CoV-2, that comprises:
(i) a VH CDR1 having an amino acid sequence of SEQ ID NO: 141, a VH CDR2 having an amino acid sequence of SEQ ID NO: 186, a VH CDR3 having an amino acid sequence of SEQ ID NO: 223, a VL CDR1 having an amino acid sequence of SEQ ID NO: 14, a VL CDR2 having an amino acid sequence of SEQ ID NO: 45, and a VL CDR3 having an amino acid sequence of SEO ID NO: 83;
(ii) a VH CDR1 having an amino acid sequence of SEQ ID NO: 137, a VH CDR2 having an amino acid sequence of SEQ ID NO: 183, a VH CDR3 having an amino acid sequence of SEQ ID NO: 223, a VL CDR1 having an amino acid sequence of SEQ ID NO: 8, a VL CDR2 having an amino acid sequence of SEQ ID NO: 38, and a VL CDR3 having an amino acid sequence of SEQ ID NO: 80;
(iii) a VH CDR1 having an amino acid sequence of SEQ ID NO: 137, a VH CDR2 having an amino acid sequence of SEQ ID NO: 179, a VH CDR3 having an amino acid sequence of SEQ ID NO: 223, a VL CDR1 having an amino acid sequence of SEQ ID NO: 8, a VL CDR2 having an amino acid sequence of SEQ ID NO: 38, and a VL CDR3 having an amino acid sequence of SEO ID NO: 76: or
(iv) a VH CDR1 having an amino acid sequence of SEQ ID NO: 137, a VH CDR2 having an amino acid sequence of SEQ ID NO: 217, a VH CDR3 having an amino acid sequence of SEQ ID NO: 222, a VL CDR1 having an amino acid sequence of SEQ ID NO: 8, a VL CDR2 having an amino acid sequence of SEQ ID NO: 38, and a VL CDR3 having an amino acid sequence of SEQ ID NO: 114.