IP Library › Granted Patent US 12,222,345
Granted Patent B2
US 12,222,345 · App. 15/931,928 · Granted Feb 11, 2025

System for imaging captured cells

Inventors: Kalyan Handique (Ann Arbor, MI); Kyle Gleason (Brighton, MI); Christopher Siemer (Ypsilanti, MI); Priyadarshini Gogoi (Ann Arbor, MI); Saedeh Sepehri Javdani (Ypsilanti, MI); Gene Parunak (Plymouth, MI); Aaron Kehrer (Plymouth, MI); Jon Meines (Plymouth, MI)
Assignee: BIO-RAD LABORATORIES, INC.
G01N33/4833G01N21/253G01N21/6428G01N21/6458G01N21/6486G01N21/76G01N33/48735G02B21/06G02B21/26A61B5/00G01N2021/6439
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 12,222,345
App. No.
15/931,928
Granted
Feb 11, 2025
Kind
B2
Abstract

A system for imaging captured cells comprising: an illumination module configured to illuminate a target object; a platform configured to position the target object in relation to the illumination module; a filter module configured to filter light transmitted to the target object and/or to filter light received from the target object, an optical sensor configured to receive light from the target object and to generate image data; and a focusing and optics module configured to manipulate light transmitted to the optical sensor. The system can further comprise one or more of: a control system configured to control at least one of the illumination module, the platform, the focusing and optics module, the filter module, and the optical sensor; a tag identifying system configured to identify and communicate tag information from system elements; a thermal control module configured to adjust temperature parameters of the system; and an image stabilization module.

Claims (19)

1. A method comprising:

receiving an imaging substrate at a platform positioned between: a first light source configured to transmit light toward the imaging substrate from a first direction and a second light source configured to transmit light toward the imaging substrate from a second direction different from the first direction; and

generating an image dataset from the imaging substrate, by way of a lens actuator coupled to a lens configured to transmit light from a target object toward an optical sensor, the target object comprising a tag having one or more features coupled to the imaging substrate, wherein the tag is defined at a surface of the imaging substrate and,

wherein generating the image dataset comprises:

actuating the platform relative to a first axis perpendicular to a plane defined by the platform, into a first configuration that aligns the imaging substrate into alignment with the first light source, the second light source, and the lens, and

adjusting operation of the lens actuator, thereby automatically focusing the lens relative to the target object by way of the tag.

2. The method of claim 1 , wherein the tag encodes an address associated with a region of the imaging substrate, the method further comprising detecting the tag and extracting the address.

3. The method of claim 1 , wherein the tag comprises an arrangement of characters, and the one or more features of the tag comprises at least one of: a relative distance between characters of the arrangement of characters, a character color, a character intensity, and a character shape.

4. The method of claim 1 , wherein the tag comprises one or more non-visible spectrum features.

5. The method of claim 1 , wherein the tag comprises a fiducial with one or more depth features coupled to the imaging substrate, the method further comprising autofocusing the lens relative to a sample component retained at the imaging substrate based upon focusing relative to the fiducial.

6. The method of claim 1 , wherein the imaging substrate comprises a set of tags, including the tag, distributed across the imaging substrate proximal a sample-containing region of the imaging substrate, wherein each of the set of tags encodes a unique address associated with a portion of the sample-containing region.

7. The method of claim 1 , wherein the imaging substrate comprises an inlet, one or more outlets, and a set of chambers in fluid communication with the inlet and the outlet, the set of chambers configured to receive a set of target particles of a sample at the inlet and capture target particles at the set of chambers in single-particle format.

8. The method of claim 7 , wherein the imaging substrate comprises a set of tags, including the tag, distributed across the imaging substrate proximal the set of chambers, wherein each of the set of tags encodes a unique address associated with a subset of the set of chambers and comprises one or more features for focusing an imaging system relative to the set of chambers.

9. The method of claim 1 , wherein generating the image dataset comprises transmitting light through an excitation filter of a filter subsystem, toward the imaging substrate, and transmitting light from the imaging substrate through an emission filter of the filter subsystem, toward the optical sensor.

10. The method of claim 9 , wherein generating the image dataset further comprises rotating the filter subsystem about a second axis, into a second configuration that positions at least one of the excitation filter and the emission filter of the filter subsystem into alignment with at least one of the first light source and the second light source.

11. The method of claim 9 , further comprising exciting contents of the imaging substrate and filtering emitted light from contents of the imaging substrate with a set of excitation filters and a set of emission filters of the filter subsystem, comprising filters for at least one of: HEX-based assays, FAM-based assays, ROX-based assays, and Cy5-based assays.

12. The method of claim 1 , wherein generating the image dataset further comprises tilting the platform, thereby generating a distribution of focal lengths across the platform relative to the lens, and performing a calibration operation, based upon a focal length of the distribution of focal lengths that provides a desired contrast level.

13. The method of claim 1 , further comprising adjusting thermal parameters of at least one of the imaging substrate and a sample retained at the imaging substrate, with a thermal control subsystem in thermal communication with the imaging substrate at the platform.

14. The method of claim 1 , wherein the lens actuator comprises a lens selector and a translation stage coupled to an optical shaft concentrically aligned with the lens by way of the lens selector, the method further comprising vertically translating the lens to facilitate autofocusing of the target object.

Assignments (4)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 5, 2020
From: CELSEE, INC.
To: BIO-RAD LABORATORIES, INC.
Reel/Frame 054269/0742 →
CHANGE OF NAME Recorded Sep 24, 2020
From: CELSEE DIAGNOSTICS, INC.
To: CELSEE, INC.
Reel/Frame 053881/0555 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 31, 2020
From: HANDIQUE, KALYAN; GLEASON, KYLE; SIEMER, CHRISTOPHER; GOGOI, PRIYADARSHINI; PARUNAK, GENE; KEHRER, AARON; MEINES, JON; JAVDANI, SAEDEH SEPEHRI
To: DENOVO SCIENCES, INC.
Reel/Frame 053372/0602 →
CHANGE OF NAME Recorded Jul 31, 2020
From: DENOVO SCIENCES, INC.
To: CELSEE DIAGNOSTICS, INC.
Reel/Frame 053377/0065 →
Continuity (7)
Continuation 16589778 · Oct 1, 2019
Continuation 15430833 · Feb 13, 2017
Continuation 15199245 · Jun 30, 2016
Continuation 14208458 · Mar 13, 2014
Provisional Application 61902431 · Nov 11, 2013
Provisional Application 61779090 · Mar 13, 2013
Related Publication 20200271637A1 · Aug 27, 2020
References Cited (306)
US 4475411A · Wellerfors · 1984 [cited by applicant]
US 4551435A · Liberti et al. · 1985 [cited by applicant]
US 4710635A · Chupp · 1987 [cited by applicant]
US 5266269A · Niiyama et al. · 1993 [cited by applicant]
US 5281540A · Merkh et al. · 1994 [cited by applicant]
US 5491343A · Brooker · 1996 [cited by applicant]
US 5541064A · Bacus et al. · 1996 [cited by applicant]
US 5547849A · Baer et al. · 1996 [cited by applicant]
US 5851488A · Saul et al. · 1998 [cited by applicant]
US 5883370A · Walker et al. · 1999 [cited by applicant]
US 5888370A · Becker et al. · 1999 [cited by applicant]
US 5993630A · Becker et al. · 1999 [cited by applicant]
US 5993632A · Becker et al. · 1999 [cited by applicant]
US 6016712A · Warden et al. · 2000 [cited by applicant]
US 6127177A · Toner et al. · 2000 [cited by applicant]
US 6133030A · Bhatia et al. · 2000 [cited by applicant]
US 6150180A · Parce et al. · 2000 [cited by applicant]
US 6174683B1 · Hahn · 2001 [cited by applicant]
US 6221663B1 · Bhatia et al. · 2001 [cited by applicant]
US 6228624B1 · Terstappen · 2001 [cited by applicant]
US 6281008B1 · Komai et al. · 2001 [cited by applicant]
US 6287832B1 · Becker et al. · 2001 [cited by applicant]
US 6365362B1 · Terstappen et al. · 2002 [cited by applicant]
US 6410724B1 · Dejean et al. · 2002 [cited by applicant]
US 6433134B1 · Patron et al. · 2002 [cited by applicant]
US 6525997B1 · Narayanaswami et al. · 2003 [cited by applicant]
US 6563634B2 · Shimada et al. · 2003 [cited by applicant]
US 6613525B2 · Nelson et al. · 2003 [cited by applicant]
US 6623983B1 · Terstappen et al. · 2003 [cited by applicant]
US 6641708B1 · Becker et al. · 2003 [cited by applicant]
US 6645731B2 · Terstappen et al. · 2003 [cited by applicant]
US 6692952B1 · Braff et al. · 2004 [cited by applicant]
US 6790330B2 · Gascoyne et al. · 2004 [cited by applicant]
US 6821484B1 · Gregersen · 2004 [cited by applicant]
US 6861259B2 · Columbus · 2005 [cited by applicant]
US 6866823B2 · Wardlaw · 2005 [cited by applicant]
US 6960449B2 · Wang et al. · 2005 [cited by applicant]
US 7008789B2 · Gambini et al. · 2006 [cited by applicant]
US 7035170B2 · Narayanaswami et al. · 2006 [cited by applicant]
US 7046357B2 · Weinberger et al. · 2006 [cited by applicant]
US 7148492B2 · Loney et al. · 2006 [cited by applicant]
US 7172866B2 · Hahn et al. · 2007 [cited by applicant]
US 7198901B1 · Rachlin · 2007 [cited by applicant]
US 7217520B2 · Tsinberg et al. · 2007 [cited by applicant]
US 7238521B2 · Hahn et al. · 2007 [cited by applicant]
US 7248352B2 · Hamamatsu et al. · 2007 [cited by applicant]
US 7258990B2 · Falcovitz-Gerassi et al. · 2007 [cited by applicant]
US 7266777B2 · Scott et al. · 2007 [cited by applicant]
US 7294468B2 · Bell et al. · 2007 [cited by applicant]
US 7316897B2 · Bisconte et al. · 2008 [cited by applicant]
US 7332288B2 · Terstappen et al. · 2008 [cited by applicant]
US 7338760B2 · Gong et al. · 2008 [cited by applicant]
US 7354389B2 · Kureshy et al. · 2008 [cited by applicant]
US 7439062B2 · Bhatt et al. · 2008 [cited by applicant]
US 7449558B2 · Yao et al. · 2008 [cited by applicant]
US 7449778B2 · Sander · 2008 [cited by applicant]
US 7507528B2 · Albert et al. · 2009 [cited by applicant]
US 7588672B2 · Unger et al. · 2009 [cited by applicant]
US 7595157B2 · Tsinberg · 2009 [cited by applicant]
US 7597528B2 · Rodi · 2009 [cited by applicant]
US 7604777B2 · Columbus · 2009 [cited by applicant]
US 7638464B2 · Fagnani et al. · 2009 [cited by applicant]
US 7695956B2 · Tsinberg et al. · 2010 [cited by applicant]
US 7704322B2 · Hansen et al. · 2010 [cited by applicant]
US 7710563B2 · Betzig et al. · 2010 [cited by applicant]
US 7738320B2 · Taha · 2010 [cited by applicant]
US 7763704B2 · Ding et al. · 2010 [cited by applicant]
US 7815863B2 · Kagan et al. · 2010 [cited by applicant]
US 7858757B2 · Hollmann et al. · 2010 [cited by applicant]
US 7863012B2 · Rao et al. · 2011 [cited by applicant]
US 7901950B2 · Connelly et al. · 2011 [cited by applicant]
US 7964349B2 · Bell et al. · 2011 [cited by applicant]
US 8008032B2 · Forsyth et al. · 2011 [cited by applicant]
US 8013298B2 · Khursheed · 2011 [cited by applicant]
US 8021614B2 · Huang et al. · 2011 [cited by applicant]
US 8103080B2 · George et al. · 2012 [cited by applicant]
US 8105769B2 · Bell et al. · 2012 [cited by applicant]
US 8105780B2 · Su et al. · 2012 [cited by applicant]
US 8131053B2 · Ortyn et al. · 2012 [cited by applicant]
US 8158410B2 · Tang et al. · 2012 [cited by applicant]
US 8174698B2 · Peter et al. · 2012 [cited by applicant]
US 8175371B2 · George et al. · 2012 [cited by applicant]
US 8186913B2 · Toner et al. · 2012 [cited by applicant]
US 8232112B2 · Willson et al. · 2012 [cited by applicant]
US 8252517B2 · Thomas et al. · 2012 [cited by applicant]
US 8293524B2 · Ionescu-Zanetti et al. · 2012 [cited by applicant]
US 8304230B2 · Toner et al. · 2012 [cited by applicant]
US 8329422B2 · Rao et al. · 2012 [cited by applicant]
US 8372579B2 · Toner et al. · 2013 [cited by applicant]
US 8372584B2 · Shoemaker et al. · 2013 [cited by applicant]
US 8406498B2 · Ortyn et al. · 2013 [cited by applicant]
US 8465916B2 · Bell et al. · 2013 [cited by applicant]
US 8628923B2 · Hamilton et al. · 2014 [cited by applicant]
US 8658418B2 · Daridon · 2014 [cited by applicant]
US 8680025B2 · Cooney · 2014 [cited by applicant]
US 8730479B2 · Ness et al. · 2014 [cited by applicant]
US 8765454B2 · Zhou et al. · 2014 [cited by applicant]
US 8771609B2 · Ehben et al. · 2014 [cited by applicant]
US 8802367B2 · Taniguchi et al. · 2014 [cited by applicant]
US 8936945B2 · Handique et al. · 2015 [cited by applicant]
US 8986988B2 · Karnik et al. · 2015 [cited by applicant]
US 9103754B2 · Handique et al. · 2015 [cited by applicant]
US 9110026B2 · Collins · 2015 [cited by applicant]
US 9133499B2 · Di Carlo et al. · 2015 [cited by applicant]
US 9145540B1 · Deutsch et al. · 2015 [cited by applicant]
US 9174216B2 · Handique et al. · 2015 [cited by applicant]
US 9188586B2 · Fan et al. · 2015 [cited by applicant]
US 9194001B2 · Brenner · 2015 [cited by applicant]
US 9200245B2 · Deutsch et al. · 2015 [cited by applicant]
US 9201060B2 · Voldman et al. · 2015 [cited by applicant]
US 9249459B2 · Hamilton et al. · 2016 [cited by applicant]
US 9260753B2 · Xie et al. · 2016 [cited by applicant]
US 9290808B2 · Fodor et al. · 2016 [cited by applicant]
US 9290809B2 · Fodor et al. · 2016 [cited by applicant]
US 9304065B2 · Fowler et al. · 2016 [cited by applicant]
US 9315768B2 · Vrouwe et al. · 2016 [cited by applicant]
US 9315857B2 · Fu et al. · 2016 [cited by applicant]
US 9329170B2 · Clarke et al. · 2016 [cited by applicant]
US 9364829B2 · Heid et al. · 2016 [cited by applicant]
US 9404864B2 · Handique et al. · 2016 [cited by applicant]
US 9410201B2 · Hindson et al. · 2016 [cited by applicant]
US 9429500B2 · Fowler et al. · 2016 [cited by applicant]
US 9506845B2 · Fowler et al. · 2016 [cited by applicant]
US 9507609B2 · Glazer et al. · 2016 [cited by applicant]
US 9513195B2 · Handique et al. · 2016 [cited by applicant]
US 9567645B2 · Fan et al. · 2017 [cited by applicant]
US 9567646B2 · Fan et al. · 2017 [cited by applicant]
US 9598736B2 · Fan et al. · 2017 [cited by applicant]
US 9610581B2 · Handique et al. · 2017 [cited by applicant]
US 9637799B2 · Fan et al. · 2017 [cited by applicant]
US 9701998B2 · Hindson et al. · 2017 [cited by applicant]
US 9707562B2 · Handique et al. · 2017 [cited by applicant]
US 9708659B2 · Fodor et al. · 2017 [cited by applicant]
US 9757707B2 · Husain et al. · 2017 [cited by applicant]
US 9802193B2 · Handique et al. · 2017 [cited by applicant]
US 9840732B2 · Anderson et al. · 2017 [cited by applicant]
US 9845502B2 · Fodor et al. · 2017 [cited by applicant]
US 9850483B2 · Clarke et al. · 2017 [cited by applicant]
US 9952126B2 · Fowler et al. · 2018 [cited by applicant]
US 9995662B2 · Husain et al. · 2018 [cited by applicant]
US 10466160B2 · Handique et al. · 2019 [cited by applicant]
US 20020009759A1 · Terstappen et al. · 2002 [cited by applicant]
US 20020028431A1 · Julien · 2002 [cited by applicant]
US 20020036142A1 · Gascoyne et al. · 2002 [cited by applicant]
US 20020036823A1 · Shimada et al. · 2002 [cited by applicant]
US 20020098535A1 · Wang et al. · 2002 [cited by applicant]
US 20020109838A1 · Columbus · 2002 [cited by applicant]
US 20020119482A1 · Nelson et al. · 2002 [cited by applicant]
US 20020192808A1 · Gambini · 2002 [cited by examiner]
US 20030103662A1 · Finkbeiner · 2003 [cited by applicant]
US 20030129676A1 · Terstappen et al. · 2003 [cited by applicant]
US 20030138941A1 · Gong et al. · 2003 [cited by applicant]
US 20040029241A1 · Hahn et al. · 2004 [cited by applicant]
US 20040095873A1 · Russell · 2004 [cited by examiner]
US 20040106130A1 · Besemer et al. · 2004 [cited by applicant]
US 20040160599A1 · Hamamatsu et al. · 2004 [cited by applicant]
US 20040191891A1 · Tsinberg et al. · 2004 [cited by applicant]
US 20040218472A1 · Narayanaswami et al. · 2004 [cited by applicant]
US 20040229349A1 · Daridon · 2004 [cited by applicant]
US 20040239922A1 · Modlin et al. · 2004 [cited by applicant]
US 20040248318A1 · Weinberger et al. · 2004 [cited by applicant]
US 20050001176A1 · Loney et al. · 2005 [cited by applicant]
US 20050014201A1 · Deuthsch · 2005 [cited by applicant]
US 20050037343A1 · Fagnani et al. · 2005 [cited by applicant]
US 20050042685A1 · Albert et al. · 2005 [cited by applicant]
US 20050063863A1 · Columbus · 2005 [cited by applicant]
US 20050105172A1 · Hasegawa et al. · 2005 [cited by applicant]
US 20050112589A1 · Hahn et al. · 2005 [cited by applicant]
US 20050118640A1 · Kureshy et al. · 2005 [cited by applicant]
US 20050158804A1 · Yao et al. · 2005 [cited by applicant]
US 20050164236A1 · Su et al. · 2005 [cited by applicant]
US 20050181463A1 · Rao et al. · 2005 [cited by applicant]
US 20050265815A1 · Rodi · 2005 [cited by applicant]
US 20060040274A1 · Tsinberg · 2006 [cited by applicant]
US 20060040407A1 · Falcovitz-Gerassi et al. · 2006 [cited by applicant]
US 20060050142A1 · Scott et al. · 2006 [cited by applicant]
US 20060115380A1 · Kagan et al. · 2006 [cited by applicant]
US 20060128006A1 · Gerhardt et al. · 2006 [cited by applicant]
US 20060141045A1 · Bhatt et al. · 2006 [cited by applicant]
US 20060147959A1 · Bell et al. · 2006 [cited by applicant]
US 20060160243A1 · Tang et al. · 2006 [cited by applicant]
US 20060257992A1 · McDevitt et al. · 2006 [cited by applicant]
US 20060263250A1 · Blouin et al. · 2006 [cited by applicant]
US 20070025882A1 · Zuppiger et al. · 2007 [cited by applicant]
US 20070026381A1 · Huang et al. · 2007 [cited by applicant]
US 20070111302A1 · Handique et al. · 2007 [cited by applicant]
US 20070154960A1 · Connelly et al. · 2007 [cited by applicant]
US 20070161051A1 · Tsinberg et al. · 2007 [cited by applicant]
US 20070172903A1 · Toner et al. · 2007 [cited by applicant]
US 20070243523A1 · Ionescu-Zanetti et al. · 2007 [cited by applicant]
US 20070252265A1 · Sander · 2007 [cited by applicant]
US 20070264675A1 · Toner et al. · 2007 [cited by applicant]
US 20070275418A1 · Hollmann et al. · 2007 [cited by applicant]
US 20080003224A1 · Fong et al. · 2008 [cited by applicant]
US 20080014589A1 · Link et al. · 2008 [cited by applicant]
US 20080068588A1 · Hess et al. · 2008 [cited by applicant]
US 20080090239A1 · Shoemaker et al. · 2008 [cited by applicant]
US 20080096212A1 · Bell et al. · 2008 [cited by applicant]
US 20080113906A1 · Ding et al. · 2008 [cited by applicant]
US 20080124726A1 · Monforte · 2008 [cited by applicant]
US 20080182273A1 · Hansen et al. · 2008 [cited by applicant]
US 20080206751A1 · Squirrell et al. · 2008 [cited by applicant]
US 20080207615A1 · Bell et al. · 2008 [cited by applicant]
US 20080220422A1 · Shoemaker et al. · 2008 [cited by applicant]
US 20080234264A1 · Bell et al. · 2008 [cited by applicant]
US 20080240539A1 · George et al. · 2008 [cited by applicant]
US 20080317325A1 · Ortyn et al. · 2008 [cited by applicant]
US 20090014360A1 · Toner et al. · 2009 [cited by applicant]
US 20090061450A1 · Hunter · 2009 [cited by applicant]
US 20090081773A1 · Kaufman · 2009 [cited by applicant]
US 20090141593A1 · Taha · 2009 [cited by applicant]
US 20090153844A1 · Peter et al. · 2009 [cited by applicant]
US 20090215072A1 · McDevitt · 2009 [cited by examiner]
US 20090215088A1 · Forsyth et al. · 2009 [cited by applicant]
US 20090258383A1 · Kovac et al. · 2009 [cited by applicant]
US 20090317836A1 · Kuhn et al. · 2009 [cited by applicant]
US 20100120077A1 · Daridon · 2010 [cited by applicant]
US 20100127168A1 · Khursheed · 2010 [cited by applicant]
US 20100173394A1 · Colston et al. · 2010 [cited by applicant]
US 20100210009A1 · Willson et al. · 2010 [cited by applicant]
US 20100232675A1 · Ortyn et al. · 2010 [cited by applicant]
US 20100233693A1 · Kopf-Sill et al. · 2010 [cited by applicant]
US 20100261179A1 · Betley et al. · 2010 [cited by applicant]
US 20100291584A1 · Tseng et al. · 2010 [cited by applicant]
US 20100304485A1 · Karnik et al. · 2010 [cited by applicant]
US 20100304978A1 · Robbins et al. · 2010 [cited by applicant]
US 20110003380A1 · Miltenyi et al. · 2011 [cited by applicant]
US 20110005932A1 · Jovanovich et al. · 2011 [cited by applicant]
US 20110045994A1 · Voldman et al. · 2011 [cited by applicant]
US 20110053151A1 · Hansen et al. · 2011 [cited by applicant]
US 20110104718A1 · Rao et al. · 2011 [cited by applicant]
US 20110117634A1 · Halamish et al. · 2011 [cited by applicant]
US 20110143964A1 · Zhou et al. · 2011 [cited by applicant]
US 20110227558A1 · Mannion et al. · 2011 [cited by applicant]
US 20110236904A1 · Hauch et al. · 2011 [cited by applicant]
US 20110280467A1 · George et al. · 2011 [cited by applicant]
US 20110310395A1 · Tsai · 2011 [cited by examiner]
US 20120021456A1 · Levine et al. · 2012 [cited by applicant]
US 20120071355A9 · Cooney · 2012 [cited by applicant]
US 20120129190A1 · Chiu et al. · 2012 [cited by applicant]
US 20120156675A1 · Lueerssen et al. · 2012 [cited by applicant]
US 20120164679A1 · Vrouwe et al. · 2012 [cited by applicant]
US 20120194805A1 · Ness et al. · 2012 [cited by applicant]
US 20130171628A1 · Di et al. · 2013 [cited by applicant]
US 20130244906A1 · Collins · 2013 [cited by applicant]
US 20140161686A1 · Bort et al. · 2014 [cited by applicant]
US 20140173443A1 · Hawkins et al. · 2014 [cited by applicant]
US 20140212881A1 · Handique et al. · 2014 [cited by applicant]
US 20140213487A1 · Freudenthal et al. · 2014 [cited by applicant]
US 20140272965A1 · Handique et al. · 2014 [cited by applicant]
US 20140315237A1 · Masujima et al. · 2014 [cited by applicant]
US 20140357511A1 · Handique et al. · 2014 [cited by applicant]
US 20140370612A1 · Bassler et al. · 2014 [cited by applicant]
US 20150089359A1 · Brisebois · 2015 [cited by applicant]
US 20150093306A1 · Thorne et al. · 2015 [cited by applicant]
US 20150133319A1 · Fu et al. · 2015 [cited by applicant]
US 20150160931A1 · Glazer et al. · 2015 [cited by applicant]
US 20150204864A1 · Fan et al. · 2015 [cited by applicant]
US 20150299784A1 · Fan et al. · 2015 [cited by applicant]
US 20150376609A1 · Hindson et al. · 2015 [cited by applicant]
US 20160024572A1 · Shishkin et al. · 2016 [cited by applicant]
US 20160024761A1 · Korb · 2016 [cited by applicant]
US 20160053253A1 · Salathia et al. · 2016 [cited by applicant]
US 20160060621A1 · Agresti et al. · 2016 [cited by applicant]
US 20160130649A1 · Xie et al. · 2016 [cited by applicant]
US 20160199838A1 · Handique et al. · 2016 [cited by applicant]
US 20160209319A1 · Adalsteinsson et al. · 2016 [cited by applicant]
US 20160251714A1 · Conant et al. · 2016 [cited by applicant]
US 20160289669A1 · Fan et al. · 2016 [cited by applicant]
US 20160314242A1 · Schnall-Levin et al. · 2016 [cited by applicant]
US 20170044525A1 · Kaper et al. · 2017 [cited by applicant]
US 20170307502A1 · Mason et al. · 2017 [cited by applicant]
US 20170320038A1 · Husain et al. · 2017 [cited by applicant]
US 20170321252A1 · Hindson et al. · 2017 [cited by applicant]
US 20170335385A1 · Hindson et al. · 2017 [cited by applicant]
US 20170356027A1 · Hindson et al. · 2017 [cited by applicant]
US 20180030515A1 · Regev et al. · 2018 [cited by applicant]
US 20180037942A1 · Fu · 2018 [cited by applicant]
US 20180051321A1 · Hindson et al. · 2018 [cited by applicant]
US 20180080075A1 · Brenner et al. · 2018 [cited by applicant]
US 20180094298A1 · Hindson et al. · 2018 [cited by applicant]
US 20180094312A1 · Hindson et al. · 2018 [cited by applicant]
US 20180105808A1 · Mikkelsen et al. · 2018 [cited by applicant]
US 20180112266A1 · Hindson et al. · 2018 [cited by applicant]
US 20180127744A1 · Hu et al. · 2018 [cited by applicant]
US 20180127823A1 · Shekhar et al. · 2018 [cited by applicant]
US 20180274027A1 · Hindson et al. · 2018 [cited by applicant]
US 20180282804A1 · Hindson et al. · 2018 [cited by applicant]
EP 2414548A2 · 2012 [cited by applicant]
EP 2414548B1 · 2015 [cited by applicant]
JP 2006098696A · 2006 [cited by applicant]
WO 2003035909A2 · 2003 [cited by applicant]
WO 2006098696A1 · 2006 [cited by applicant]
WO 2010120818A2 · 2010 [cited by applicant]
WO 2018013723A1 · 2018 [cited by applicant]
WO 2018058073A2 · 2018 [cited by applicant]
Australian Examination Report for Application No. 2018323449, dated Feb. 25, 2020. [cited by applicant]
Guo, P. et al. Microfluidic capture and release of bacteria in a conical nanopore array. Lab Chip. Vol. 12, p. 558-561, 2012, published online Nov. 2011. [cited by applicant]
International Search Report and Written Opinion for PCT Application No. PCT/US17/62099 mailed Feb. 12, 2018. [cited by applicant]
International Search Report for PCT Application No. PCT/US2018/048353 mailed Nov. 5, 2018. [cited by applicant]
Lindstrom, Sara (Royal Institute of Technology, Stockholm, Sweden, 2009, pp. 1-80). [cited by applicant]
Seale, K. T. et al. “Mirrored pyramidal wells for simultaneous multiple vantage point microscopy.” Journal of Microscopy (2008) 232 1-6. (Year: 2008). [cited by applicant]
Sugio, Yoshihiro; et al., An agar-based on-chip neural-cell-cultivation system for stepwise control of network batter generation during cultivation, Dept. of Life Sciences, Graduate School of Arts and Sciences, Universi… [cited by applicant]
Supplemental information from Tan et al. PNAS (2007) 104. (Year: 2007). [cited by applicant]
Tan, Wei-Heang et al. “A trap-and-release integrated microfluidic system for dynamic microarray applications.” PNAS (2007) 104 1146-1151. (Year: 2007). [cited by applicant]
“International Preliminary Report on Patentability for PCT Application No. PCT/US17/62099 mailed May 31, 2019.” [cited by applicant]