Novel Treatment Method for Cockayne Syndrome
Disclosed is a method for treating of Cockayne Syndrome (CS). Specifically, the invention pertains to a method for the extracorporeal treatment of a body fluid by removing the body fluid from a living body diseased with CS, and applying a targeted antibody conjugated to a moiety to at least one Cockayne Syndrome antigen in the body fluid, creating an antibody-antigen moiety complex, removing antibody-antigen moiety complex from the body fluid, and returning the purified body fluid to the body.
1 . A method for the extracorporeal treatment of a body fluid carrying at least one Cockayne Syndrome Targeted Antigen (CSTA) comprising:
treating the body fluid carrying at least one unbound CSTA with at least one CSTA targeted antibody in an amount sufficient to bind all the at least one unbound CSTA forming CSTA target antibody-CSTA complexes, wherein the at least one CSTA targeted antibody is conjugated with a moiety;
removing the CSTA targeted antibody-CSTA complexes from the body fluid; and
identifying any remaining CSTA targeted antibody-CSTA complexes.
2 . The method of claim 1 wherein the CSTA is at least one member selected from the group consisting of:
death-associated protein 1 (DAP1), calpain, p21, mammalian target of rapamycin (mTOR), insulin growth factor-1 (IGF-1), lipofuscin, p16 (p16INK4a), cyclin-dependent kinase inhibitor 2A (CDKN2A), Senescence associated β-gal (SA-β-gal), promyelocytic leukemia (PML) protein, transforming growth factor β (TGF-β), interleukin-6 (IL-6), indoleamine 2,3-dioxygenase, soluble tumor necrosis factor-receptor 55 (sTNF-R55), soluble tumor necrosis factor-receptor 75 (sTNF-R75), Progerin, oxygen and nitrogen based free radicals: (superoxide, nitric oxide, hydroxyl radical, perioxynitrite, nitrosoperoxycarbonate, hydrogen peroxide, hypochlorite); malondialdehyde (MDA, propanedial); tumor necrosis factor-α (TNF-α), and mitogen activated protein kinases (MAPK).
3 . The method of claim 1 wherein the moiety is albumin.
4 . The method of claim 1 wherein the moiety is a macromolecule; the macromolecule having diameter ranging from 0.00001 mm-1.00 mm.
5 . The method of claim 1 wherein the moiety is iron.
6 . The method of claim 1 wherein removing the CSTA targeted antibody-CSTA complexes from the body fluid is done using MARS.
7 . The method of claim 1 wherein removing the CSTA targeted antibody-CSTA complexes from the body fluid is done using micro screens.
8 . The method of claim 1 wherein removing the CSTA targeted antibody-CSTA complexes from the body fluid is done using continuous renal replacement therapy.
9 . The method of claim 1 wherein removing the CSTA targeted antibody-CSTA complexes from the body fluid is done using zeolite.
10 . The method of claim 1 wherein removing the CSTA targeted antibody-CSTA complexes from the body fluid is done using membranes that filter for specific molecular sizes.
11 . The method of claim 1 wherein removing the CSTA targeted antibody-CSTA complexes from the body fluid is done using immunoaffinity chromatography.
12 . The method of claim 1 wherein removing the CSTA targeted antibody-CSTA complexes from the body fluid is done using gel filtration chromatography.
13 . The method of claim 1 wherein identifying any remaining CSTA targeted antibody-CSTA complexes is done by enzyme-linked immunosorbent assay (ELISA).
14 . The method of claim 1 wherein identifying any remaining CSTA targeted antibody-CSTA complexes is done by molecular weight cut-off filtration.
15 . The method of claim 1 further comprised of adding one or more therapeutic agents to the body fluid, wherein the one or more therapeutic agents are at least one member selected from the group consisting of:
recombinant ERCC1, recombinant ERCC4, recombinant ERCC5, recombinant ERCC8, recombinant ERCC6, and recombinant KIAA1530.
16 . The method of claim 1 further comprised of adding one or more therapeutic agents to the body fluid, wherein the one or more therapeutic agents are at least one member selected from the group consisting of: Lithium chloride, rapamycin, rotenone, p62 (nucleoporin 62), magnesium and coenzyme Q10, recombinant IL-10, sildenafil, amitriptyline, recombinant CS-A, recombinant CS-B, Prodarsan, calcium blockers such as diltiazem, low dose prednisolone and/or deflazacort, Vitamin D3, Vitamin C, and Vitamin E.
17 . The method of claim 1 further comprised of adding one or more therapeutic agents to the body fluid, wherein the therapeutic agents are autophagy agonists.
18 . The method of claim 1 further comprised of adding or more therapeutic agents to the body fluid, wherein the therapeutic agents are autophagy inducers.
19 . The method of claim 1 further comprising treating the body fluid with antibodies targeted against miR222 and miR223.