Cuvette for detecting bacteria and determining their susceptibility to antibiotics

1. A method of determining susceptibility of bacteria to different chemo-effecter agents by use of a system having a light source for generating an input light beam that transmits along an input beam axis and enters a cuvette containing a biological fluid having the bacteria, the system further having a light detector for receiving light forwardly scattered from the biological fluid, the method comprising:

mixing the biological fluid having the bacteria with a first chemo-effecter agent in a first cuvette to form a first mixed fluid sample, the first cuvette having a first entry window and a first exit window that are transparent and made of a plastic material, the first exit window being located on the opposite side of the first cuvette from the first entry window;

mixing the biological fluid having the bacteria with a second chemo-effecter agent in a second cuvette to form a second mixed fluid sample, the second cuvette having a second entry window and a second exit window that are transparent and made of a plastic material, the second exit window being located on the opposite side of the second cuvette from the second entry window;

aligning the first cuvette in the system such that the first entry window and the first exit window are aligned with the input beam axis of the input light beam;

illuminating a portion of the first mixed fluid sample within the first cuvette with the input light beam to create forward-scattered light exiting the first exit window in locations spaced apart from the input beam axis;

at a location behind the first exit window and in front of the light detector in the direction of the input beam axis, obscuring a remaining portion of the input light beam that exits the first exit window of the first cuvette to inhibit an effect of the input light beam on the light detector;

repeatedly receiving a first set of forward-scattered signals at the light detector due to the forward-scattered light from first mixed fluid sample;

aligning the second cuvette in the system such that the second entry window and the second exit window are aligned with the input beam axis of the input light beam;

illuminating a portion of the second mixed fluid sample within the second cuvette with the input light beam to create forward-scattered light exiting the second exit window in locations spaced apart from the input beam axis;

at a location behind the second exit window and in front of the light detector in the direction of the input beam axis, obscuring a remaining portion of the input light beam that exits the second exit window of the second cuvette to inhibit an effect of the input light beam on the light detector;

repeatedly receiving a second set of forward-scattered signals at the light detector due to the forward-scattered light from second mixed fluid sample; and

comparing the first set of forward-scattered signals and the second set of forward-scattered signals to determine the susceptibility of the bacteria to the first chemo-effector agent and the second chemo-effector agent.

2. The method of claim 1 , further including regulating the temperature of the first cuvette and the second cuvette while repeatedly receiving the first and second sets of forward-scattered signals.

3. The method of claim 2 , wherein the regulating the temperature includes inducing a temperature gradient in the biological sample.

4. The method of claim 1 , wherein the repeatedly receiving the first and second sets of forward-scattered signals includes measuring the intensity of forward scatted signals.

5. The method of claim 1 , further including filtering the biological fluid prior to placing the biological fluid in the first and second cuvettes.

6. The method of claim 5 , wherein the biological fluid is urine.

7. The method of claim 1 , wherein the biological fluid is urine.

8. The method of claim 7 , wherein the first and second sets of forward-scattered signals are indicative of a change in concentration level of the bacteria.

9. The method of claim 1 , wherein the first and second sets of forward-scattered signals are indicative of a change in concentration level of the bacteria.

10. The method of claim 9 , wherein the change in concentration level of the bacteria indicates the bacterial susceptibility to the first chemo-effector agent and the second chemo-effector agent.

11. The method of claim 1 , wherein the mixing includes manually placing the first chemo-effector agent into the first cuvette.

12. The method of claim 1 , further including placing the biological sample into a third cuvette that serves as a control sample, wherein the method further includes performing the (i) aligning, (ii) illuminating, (iii) obscuring, and (iv) repeatedly receiving acts on the control sample within the third cuvette.

13. The method of claim 1 , wherein each of the windows has an optical quality defined by a scratch/dig number that is 40/20 or lower.

14. The method of claim 1 , wherein the second chemo-effector agent is different from the first chemo-effector agent.

15. The method of claim 1 , wherein the second chemo-effector agent is the same agent as the first chemo-effector agent, the second chemo-effector agent being at a different concentration than the first chemo-effector agent.

16. The method of claim 1 , wherein the first chemo-effector agent is an antibiotic agent.

17. The method of claim 16 , wherein the second chemo-effector agent is an antibiotic agent.

18. The method of claim 1 , wherein each of the first and second cuvettes includes an internal compartment for containing, respectively, the first and second chemo-effector agents.

19. The method of claim 18 , wherein each of the first and second cuvettes includes a removable cover adjacent to the internal compartment.

20. The method of claim 1 , wherein the input light beam is derived from a laser.