Smear staining machine
View Patent ↗A smear staining machine and a smearing control method and device thereof. The viscosity of the test sample is used for guiding the configuration of at least on smearing parameter. Since the viscosity of the test sample presents multiple influences of many effecting parameters, it is more suitable for presenting the characteristics of the test sample. Therefore, better smearing effect could be acquired by referring the viscosity of the test sample to get the smearing parameter.
1. A smear staining machine, comprising:
a sampling pipeline configured to draw a test sample;
an injector configured to drive the sampling pipeline to draw or discharge the test sample;
a smearing mechanism configured to drop the test sample on a slide and extending the test sample as a film according to a smearing parameter;
a detector, configured on the sampling pipeline and configured to detect a viscosity characteristic of the test sample flowing through the pipeline under a preset condition, wherein the viscosity characteristic is a physical quantity influenced by the viscosity of the test sample when the test sample flows, and the physical quantity is a function of viscosity, wherein the detector comprises a pressure sensor and the viscosity characteristic is a pressure data detected by the pressure sensor; and
a processor, electrically connected with the injector, the smearing mechanism and the detector respectively, wherein the processor is configured to control the injector to drive a drawing action or a discharging action of the sampling pipeline, receive the viscosity characteristic of the test sample from the detector, acquire the smearing parameter according to the viscosity characteristic of the test sample and control the smearing mechanism to smear according to the smearing parameter.
2. The smear staining machine of claim 1 , wherein the processor is configured to calculate a viscosity ratio between the reference fluid and the test sample according to the output of the pressure sensor and determine the smearing parameter according to an environment temperature and the viscosity ratio.
3. The smear staining machine of claim 1 , wherein the sampling pipeline is further configured to draw a reference fluid;
the detector is further configured to detect a viscosity characteristic of the reference fluid flowing through the pipeline under a preset condition;
the processor is further configured to:
control the injector to drive the sampling pipeline to draw or discharge the reference fluid and the test sample;
compare the viscosity characteristic of the test sample with the viscosity characteristic of the reference fluid flowing through the pipeline under a same preset condition to generate a comparison result;
calculate a relative viscosity of the test sample according to the comparison result and a known apparent viscosity of the reference fluid; and
determine the smearing parameter based on the relative viscosity, wherein the relative viscosity of the test sample is a viscosity that relative to the reference fluid having the known apparent viscosity under a smearing temperature.
4. The smear staining machine of claim 3 , further comprising:
a temperature sensor, configured on the sampling pipeline to detect the temperature in the pipeline when a fluid flows through the pipeline and output the temperature in the pipeline to the processor.
5. The smear staining machine of claim 4 , wherein the processor is configured to:
calculate a viscosity ratio between the reference fluid and the test sample according to the outputs of the pressure sensor and the temperature sensor;
calculate a relative viscosity of the test sample according to a known apparent viscosity of the reference fluid under a test temperature; and
determine the smearing parameter according to the relative viscosity, the relative viscosity of the test sample is a viscosity relative to that of the reference fluid having the known apparent viscosity under the smearing temperature.
6. The smear staining machine of claim 5 , wherein the processor is configured to:
control the injector to drive the sampling pipeline to draw the reference fluid with a predetermined volume when a smear operation is needed, and receive the pressure data of the reference fluid detected by the pressure sensor and a first temperature in the pipeline detected by the temperature sensor; and
control the injector to drive the sampling pipeline to draw the test sample with a predetermined volume, receive the pressure data detected by the pressure sensor and calculate the relative viscosity of the test sample according to a relation of the pressure data of the test sample and the reference fluid and the viscosity of the test sample and the reference fluid.
7. The smear staining machine of claim 5 , wherein the reference fluid comprises a portion of reference gas and a portion of reference liquid;
before a smear operation, the processor is configured to control the injector to drive the sampling pipeline to respectively draw the reference gas and reference liquid with a predetermined volume, receive the pressure data of the reference gas and the reference liquid detected by the pressure sensor and the first temperature in the pipeline detected by the temperature sensor;
during the smear operation, the processor is configured to control the injector to drive the sampling pipeline to draw the test sample with a predetermined volume, receive the pressure data of the test sample detected by the pressure sensor and a second temperature in the pipeline detected by the temperature sensor; and
the processor is configured to calculate the relative viscosity of the test sample according to a relation of the pressure data of the test sample and the reference fluid and the viscosity of the test sample and the reference fluid.