Method of treating HIV-1 infection utilizing a multiepitope T cell immunogen comprising gag, pol, vif and nef epitopes
The present invention relates to novel immunogens based on overlapping peptides (OLPs) and peptides derived therefrom useful for the prevention and treatment of AIDS and its related opportunistic diseases. The invention also relates to isolated nucleic acids, vectors and host cells expressing these immunogens as well as vaccines including said immunogens.
1. A method of treating an HIV infection or a disease associated with an HIV infection in a subject in need thereof, the method comprising sequentially administering to the subject (1) a nucleic acid encoding a first immunogenic polypeptide and (2) a second immunogenic polypeptide or a nucleic acid encoding a second immunogenic polypeptide,
wherein the first immunogenic polypeptide comprises the sequences of SEQ ID NOs:1-16 adjoined by amino acid linkers,
wherein the second immunogenic polypeptide comprises the sequences of SEQ ID NOs:1-16 adjoined by amino acid linkers,
wherein the nucleic acid encoding the first immunogenic polypeptide is administered to the subject as part of an expression cassette, an expression vector, a virus, or a cell,
wherein the nucleic acid encoding the second immunogenic polypeptide is administered to the subject as part of an expression cassette, an expression vector, a virus, or a cell, and
wherein (1) the nucleic acid encoding the first immunogenic polypeptide and (2) the second immunogenic polypeptide or the nucleic acid encoding the second immunogenic polypeptide are administered in amounts effective for eliciting an immune response against HIV in the subject.
2. The method of claim 1 , wherein the subject is a human subject, and
wherein (1) the nucleic acid encoding the first immunogenic polypeptide and (2) the second immunogenic polypeptide or the nucleic acid encoding the second immunogenic polypeptide are administered to the human subject to treat AIDS, ARC, or an HIV opportunistic disease.
3. The method of claim 1 , wherein the amino acid linkers are single, dual, or triple alanine linkers, and wherein the linkers result in the formation of an AAA sequence in the junction region between adjoining sequences.
4. The method of claim 3 , wherein the first and second immunogenic polypeptides have the sequence of SEQ ID NO:99.
5. The method of claim 1 , wherein the nucleic acids encoding the first and second immunogenic polypeptides have the sequence of SEQ ID NO:100.