Device for spectroscopic detection and monitoring of biologically relevant molecules
There is a need in the point-of-care diagnostic community for an efficient and portable method for testing blood and other biological fluids that can be easily translated across multiple applications. An aspect of the invention described involves monitoring the optical properties of molecularly-mediated nanoparticle assemblies though an optically transparent and magnetically active microfluidic chip, which has recently emerged as an attractive method for biomarker detection as it is an efficient tool for monitoring the binding events that take place in a sensing assay. In one embodiment, this device is directed towards two-nanoparticle assays that rely on the assembly or disassembly of plasmonic and magnetic nanoparticles in response to a certain analyte. A further embodiment is directed to a spiral microfluidic using inertial forces to filter fluid components by size, connected to a magnetically active channel comprised of a nickel micropad array, optically transparent microchannel, and permanent magnets.
1. A magnetic channel-based SERS device comprising:
a nickel micromagnetic array, wherein the nickel micromagnet array is constructed on a glass slide through a lift off process;
a microfluidic channel; and
a magnet.
2. The device of claim 1 , wherein the magnet is a permanent magnet or an electromagnet.
3. The device of claim 1 , wherein the microfluidic channel is comprised of polydimethylsiloxane (PDMS) or polyethylene glycol, or a combination of the two.
4. The device of claim 1 , wherein the microfluidic channel is comprised of polymethylmethacrylate (PMMA).
5. The device of claim 1 , wherein the microfluidic channel is comprised of borosilicate glass.
6. A method of fluid analysis using a microfluidic chip, the method comprising:
using a microfluidic chip that utilizes magnetic properties of assay particles to house the particles and monitor their optical response on a micro nickel array, wherein the magnetic properties of the assay particles multiplies a SERS signal intensity while providing repeatable localization of the assay components.
7. The method of claim 6 , wherein multiple optically transparent microfluidic chips are fabricated on one glass slide, and wherein each microfluidic chip contains multiple micro scale nickel pads embedded within the channel and are magnetically activated by neodymium magnets on either side.
8. The method of claim 7 , further comprising stacking or cascaded microfluidic chips to form a double layer modified version, so that an inlet of the a second microfluidic chip aligns with an outlet of a first microfluidic chip for continuous flow and user-free analysis.