Sample processing apparatus and method
The invention relates to a sample processing apparatus comprising a holder for a microtiter plate comprising a plurality of microwells, optical measurement unit for measuring optical responses of samples dosed to the microwells, and a computing unit configured to analyze the optical responses in order to detect dosing failures in said plurality of microwells, and if a dosing failure has been detected in one or more of the microwells, to communicate the existence of the dosing failure to a user of the apparatus through signaling means or to store data indicative of the dosing failure to data storage means for further use. In particular, the invention relates to detecting dosing failures in before, during and after a PCR process.
1. A method of preparing a solution comprising nucleic acid synthesis reagents, the method comprising combining a plurality of components to form the solution, wherein:
the plurality of components comprises at least first and second colorants, and first and second nucleic acid synthesis reagents;
the first colorant provides a first color, the second colorant provides a second color visually distinguishable from the first color, and combining the first and second colorants results in the solution having a third color visually distinguishable from the first and second colors; and
the first and second nucleic acid synthesis reagents together comprise two or more of a polymerase, dNTPs, a primer, a magnesium salt, a nucleic acid template, a qPCR dye, and a probe for a PCR product.
2. The method of claim 1 , wherein the first and second colors have maximum peaks in their absorption spectrum separated by at least 30 nm.
3. The method of claim 1 , wherein the first or second nucleic acid synthesis reagent comprises a polymerase.
4. The method of claim 1 , wherein the first or second nucleic acid synthesis reagent comprises dNTPs.
5. The method of claim 1 , wherein the first or second nucleic acid synthesis reagent comprises a primer.
6. The method of claim 1 , wherein the first or second nucleic acid synthesis reagent comprises a magnesium salt.
7. The method of claim 1 , wherein the first or second nucleic acid synthesis reagent comprises a nucleic acid template.
8. The method of claim 1 , wherein the first or second nucleic acid synthesis reagent comprises a qPCR dye.
9. The method of claim 1 , wherein the first or second nucleic acid synthesis reagent comprises a probe for a PCR product.
10. A method of nucleic acid synthesis, comprising:
preparing a nucleic acid synthesis reaction mixture comprising nucleic acid synthesis reagents and at least first and second colorants, wherein:
the first colorant provides a first color, the second colorant provides a second color visually distinguishable from the first color, and the first and second colorants together provide a third color visually distinguishable from the first and second colors; and
the nucleic acid synthesis reagents are sufficient to perform a nucleic acid synthesis reaction; and
performing the nucleic acid synthesis reaction.
11. The method of claim 10 , wherein the first and second colors have maximum peaks in their absorption spectrum separated by at least 30 nm.
12. The method of claim 10 , wherein the nucleic acid synthesis reaction comprises PCR.
13. The method of claim 10 , wherein the nucleic acid synthesis reaction comprises quantitative PCR.
14. The method of claim 10 , wherein the nucleic acid synthesis reaction comprises reverse transcription.
15. The method of claim 10 , wherein the nucleic acid synthesis reagents comprise dNTPs.
16. The method of claim 10 , wherein the nucleic acid synthesis reagents comprise a nucleic acid template.
17. The method of claim 10 , wherein the nucleic acid synthesis reagents comprise a qPCR dye.
18. The method of claim 10 , wherein the nucleic acid synthesis reagents comprise a primer.
19. The method of claim 10 , further comprising performing electrophoresis to analyze the product of the nucleic acid synthesis reaction.
20. The method of claim 19 , wherein at least one of the colorants is detectable in gel electrophoresis.