Variant serum albumin with improved half-life and other properties
The invention provides methods and materials for making and using variant serum albumin amino acid sequences which exhibit improved properties compared to wild type serum albumin sequences. The invention further provides methods and materials for making and using fusion proteins in which the variant serum albumin amino acid sequences are fused to a therapeutic or diagnostic agent, such as a therapeutic protein, or a functional fragment or variant thereof that maintains activity, and exhibits improved properties.
1. A method of making a variant serum albumin polypeptide (VSA) having an altered binding affinity to FcRn as compared with the binding affinity of a wild type human serum albumin (HSA) to FcRn comprising:
providing a nucleic acid encoding a variant serum albumin polypeptide (VSA) comprising a sequence that has a substitution to A at a position corresponding to position 547 of SEQ ID NO: 2, a substitution to G at a position corresponding to position 505 of SEQ ID NO: 2, and is at least 90% identical but less than 100% identical to amino acid residues 380 to 585 of SEQ ID NO: 2;
introducing the nucleic acid encoding the VSA in a suitable host cell;
culturing the host cell under conditions that allow expression of the polypeptide; and
isolating the polypeptide from the host cell or from the host cell growth medium,
wherein the VSA binds to FcRn with a higher affinity as compared to the binding affinity of HSA to FcRn.
2. The method of claim 1 , wherein the VSA binds to FcRn with a K D of less than 50 nm.
3. The method of claim 1 , wherein the VSA has a longer plasma half-life than HSA.
4. The method of claim 1 , wherein the VSA comprises a sequence that has up to 10 amino acid changes in amino acid residues 380 to 585 of SEQ ID NO: 2.
5. The method of claim 1 , wherein the VSA comprises a sequence that has up to 9 amino acid changes in amino acid residues 380 to 585 of SEQ ID NO: 2.
6. The method of claim 1 , wherein the VSA comprises a sequence that has up to 8 amino acid changes in amino acid residues 380 to 585 of SEQ ID NO: 2.
7. The method of claim 1 , wherein the VSA comprises a sequence that has up to 7 amino acid changes in amino acid residues 380 to 585 of SEQ ID NO: 2.
8. The method of claim 1 , wherein the VSA comprises a sequence that has up to 6 amino acid changes in amino acid residues 380 to 585 of SEQ ID NO: 2.
9. The method of claim 1 , wherein the VSA comprises a sequence that has up to 5 amino acid changes in amino acid residues 380 to 585 of SEQ ID NO: 2.
10. The method of claim 1 , wherein the VSA comprises a sequence that has up to 4 amino acid changes in amino acid residues 380 to 585 of SEQ ID NO: 2.
11. The method of claim 1 , wherein the VSA comprises a sequence that has up to 3 amino acid changes in amino acid residues 380 to 585 of SEQ ID NO: 2.
12. A method of delivery of a recombinant protein comprising a variant serum albumin polypeptide (VSA) to a subject, the method comprising:
administering to the subject a composition comprising an isolated recombinant protein comprising a variant serum albumin polypeptide (VSA) comprising a sequence that is at least 90% identical but less than 100% identical to amino acid residues 380 to 585 of SEQ ID NO: 2, wherein the VSA comprises a mutation corresponding to position V547 to A and a mutation corresponding to position E505 to G; and
wherein the VSA has an altered binding affinity for human FcRn compared to a wild type human serum albumin (HSA).
13. The method of claim 12 , wherein VSA binds to FcRn with a K D of less than 50 nM.
14. The method of claim 12 , comprising at least one additional mutation at a position selected from V418, T420, V424, N429, M446, A449, T467, or A552.
15. The method of claim 12 , wherein the VSA comprises a sequence that differs by at least 3 but no more than 10 residues from amino acid residues 380 to 585 of SEQ ID NO: 2.
16. The method of claim 12 , further comprising administering a therapeutic agent in combination with the composition, wherein the therapeutic agent comprises a polypeptide component that is fused to the VSA.
17. The method of claim 16 , wherein the polypeptide component and the VSA are separated by a linker sequence.
18. The method of claim 16 , wherein the polypeptide component and the VSA are covalently linked by a non-peptide bond.
19. The method of claim 16 , wherein the polypeptide component and the VSA are non-covalently and stably associated.
20. The method of claim 12 , wherein the VSA comprises a sequence that is at least 90% identical to amino acid residues 380 to 585 of SEQ ID NO: 2.
21. The method of claim 12 , wherein the VSA comprises a sequence that is at least 95% identical to amino acid residues 380 to 585 of SEQ ID NO: 2.
22. The method of claim 12 , wherein the VSA comprises a sequence that is at least 96% identical to amino acid residues 380 to 585 of SEQ ID NO: 2.
23. The method of claim 12 , wherein the VSA comprises a sequence that is at least 97% identical to amino acid residues 380 to 585 of SEQ ID NO: 2.
24. The method of claim 12 , wherein the VSA comprises a sequence that is at least 98% identical to amino acid residues 380 to 585 of SEQ ID NO: 2.
25. The method of claim 12 , wherein the VSA comprises a sequence that is at least 99% identical to amino acid residues 380 to 585 of SEQ ID NO: 2.
26. A method of making a variant serum albumin polypeptide (VSA) associated therapeutic agent, the method comprising:
providing a biologically or pharmaceutically active agent; and
associating the agent with a protein comprising a VSA, which has a substitution to A at a position corresponding to position 547 of SEQ ID NO: 2, a substitution to G at a position corresponding to position 505 of SEQ ID NO: 2, and comprises a sequence that is at least 90% identical but less than 100% identical to amino acid residues 380 to 585 of SEQ ID NO: 2 to generate a VSA associated therapeutic agent.
27. The method of claim 26 , further comprising formulating the VSA associated therapeutic agent for administration to a subject.
28. The method of claim 26 , wherein the VSA comprises a sequence that has 3 to 10 amino acid changes in amino acid residues 380 to 585 of SEQ ID NO: 2.
29. A method of making a variant serum albumin polypeptide (VSA) associated diagnostic agent, the method comprising:
providing a diagnostic agent; and
associating the diagnostic agent with a protein comprising a VSA, which comprises a sequence that has a substitution to A at a position corresponding to position 547 of SEQ ID NO: 2, a substitution to G at a position corresponding to position 505 of SEQ ID NO: 2, and is at least 90% identical but less than 100% identical to amino acid residues 380 to 585 of SEQ ID NO: 2 to generate a VSA associated diagnostic agent.
30. The method of claim 29 , further comprising formulating the VSA associated diagnostic agent for administration to a subject.
31. The method of claim 29 , further comprising administering the VSA associated diagnostic agent to a subject and detecting the VSA associated diagnostic agent.
32. The method of claim 31 , wherein the subject is imaged.
33. The method of claim 29 , wherein the VSA comprises a sequence that has 3 to 10 amino acid changes in amino acid residues 380 to 585 of SEQ ID NO: 2.